Níveis de arginase, óxido nítrico e o efeito da pge2 na produção de tnf-α em linfonodo de cães com leishmaniose visceral
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Date
Authors
Advisor
Lima, Valéria Marçal Felix de 
Coadvisor
Graduate program
Ciência Animal - FMVA
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Universidade Estadual Paulista (Unesp)
Type
Master's thesis
Access right
Acesso aberto
Abstract
Visceral leishmaniasis (VL) is a chronic disease that can be fatal to humans and dogs. The disease is caused by the intracellular parasite Leishmania infantum and is transmitted by the bite of the sandfly (phlebotomines). In dogs, VL is observed as an intense chronic inflammatory reaction in the liver, spleen, skin, bone marrow and lymph nodes. Arginase activity is important in VL because an increase of this enzyme may contribute to the multiplication of the parasite and a reduction of nitric oxide (NO) synthesis, predisposing a macrophage to infection. Prostaglandin E2 (PGE2) can play a regulatory role in the production of tumor necrosis factor (TNF-α) and interleukin-10 (IL-10), however, there have been no studies in dogs with LV. This study aimed to evaluate the arginase activity in adherent macrophages cultivated from the lymph nodes of 18 healthy and 23 naturally infected dogs and to examine levels of NO and PGE2 in the supernatant of these cultures. The regulatory effect of PGE2 on the production of TNF-α and IL-10 was also evaluated in supernatants of total lymph node leukocytes cultures. These results help to clarify the mechanisms of the immune response in CVL.
Description
Keywords
Cães, Leishmaniose visceral, Leishmania, Zoonoses, Oxido nítrico, Gânglios linfáticos, Fator de necrose de tumor, Indomethacin
Language
Portuguese
Citation
VENTURIN, Gabriela Lovizutto. Níveis de arginase, óxido nítrico e o efeito da pge2 na produção de tnf-α em linfonodo de cães com leishmaniose visceral. 2015. 59 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015.
