Publicação: Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
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Data
2012-01-01
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Sociedade Brasileira de Genética
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Resumo
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.
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Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 1, p. 122-125, 2012.
