Publicação:
Proinflammatory profile of in vitro monocytes in the ageing is affected by lymphocytes presence

dc.contributor.authorPinke, Karen Henriette
dc.contributor.authorCalzavara, Bruno
dc.contributor.authorFaria, Patricia Freitas
dc.contributor.authordo Nascimento, Magda Paula Pereira
dc.contributor.authorVenturini, James [UNESP]
dc.contributor.authorLara, Vanessa Soares
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:40Z
dc.date.available2014-05-27T11:29:40Z
dc.date.issued2013-06-08
dc.description.abstractBackground: Aging is associated with complex and constant remodeling of the immune function, resulting in an increasing susceptibility to infection and others diseases. The infections caused by Gram-negative microorganisms, present in nursing homes and hospitals, constitute one of the most common infections in the elderly, and are mainly combated by innate immune cells. Although the functions of innate immunity seem more preserved during aging than of adaptive immune mechanisms, two systems operate in an integrated way in the body, so that injury in one part of the immune system inevitably affects the other as they are part of a defensive network. The aim of this study was to investigate the in vitro production of proinflammatory (TNF-α, IL-6, IL-1β, CXCL-8 and MCP-1) and anti-inflammatory (TGF-β and IL-10) cytokines by monocytes, stimulated or not (basal) with lipopolysaccharide, from healthy young and elderly subjects. By means of PBMCs, we also studied if cytokine profile is altered in these different patient groups, in the presence of lymphocytes, under the same experimental conditions.Results: The monocytes from elderly presented higher basal production of TNF-α, MCP-1 and lower of TGF-β than young monocytes. PBMC showed similar cytokines production, irrespective age or stimulation presence. In the presence of lymphocytes, the spontaneous production of IL-10 was higher and of TGF-β was lower than monocytes, regardless of age. After LPS-stimulation, the presence of lymphocytes resulted in increased IL-6, IL-1β, MCP-1 and IL-10 and decreased CXCL-8 and TGF-β in comparison to pure culture of monocytes from young patients. With age, the same differences were observed, except for CXCL-8 and TGF-β which production was the same between monocytes and PBMC stimulated with LPS.Conclusion: These findings reinforce the systemic state of inflamm-aging frequently reported in elderly and considered a factor of susceptibility to numerous diseases. Still, the cytokine production from just monocytes of the elderly showed alterations, while in the lymphocyte presence not, suggesting an immunomodulator role of lymphocytes on monocytes. In addition, the differences between the production patterns by LPS-stimulated PBMC between young and elderly volunteers can be related with an imbalance in response against Gram-negative bacteria in throughout life. © 2013 Pinke et al.; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Stomatology Bauru School of Dentistry University of São Paulo, Al. Dr. Octávio Pinheiro Brisola, 9-75, 17012-901 Bauru, SP
dc.description.affiliationLaboratory of Experimental Immunology Department of Biological Sciences Faculty of Science, São Paulo State University, Av Eng Luiz Edmundo C, 14-01, 17033-360 Bauru, SP
dc.description.affiliationUnespLaboratory of Experimental Immunology Department of Biological Sciences Faculty of Science, São Paulo State University, Av Eng Luiz Edmundo C, 14-01, 17033-360 Bauru, SP
dc.identifierhttp://dx.doi.org/10.1186/1742-4933-10-22
dc.identifier.citationImmunity and Ageing, v. 10, n. 1, 2013.
dc.identifier.doi10.1186/1742-4933-10-22
dc.identifier.file2-s2.0-84878652553.pdf
dc.identifier.issn1742-4933
dc.identifier.scopus2-s2.0-84878652553
dc.identifier.urihttp://hdl.handle.net/11449/75623
dc.identifier.wosWOS:000327221500001
dc.language.isoeng
dc.relation.ispartofImmunity and Ageing
dc.relation.ispartofjcr4.019
dc.relation.ispartofsjr1,218
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCytokines
dc.subjectImmunosenescence
dc.subjectInflamm-aging
dc.subjectMonocytes
dc.subjectPBMC
dc.subjectinterleukin 10
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectinterleukin 8
dc.subjectlipopolysaccharide
dc.subjectmonocyte chemotactic protein 1
dc.subjecttransforming growth factor beta
dc.subjecttumor necrosis factor alpha
dc.subjectadult
dc.subjectaged
dc.subjectaging
dc.subjectcell stimulation
dc.subjectcytokine production
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectinflammatory cell
dc.subjectlymphocyte
dc.subjectmonocyte
dc.subjectperipheral blood mononuclear cell
dc.subjectpriority journal
dc.titleProinflammatory profile of in vitro monocytes in the ageing is affected by lymphocytes presenceen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dspace.entity.typePublication
unesp.author.orcid0000-0001-8716-1250[1]
unesp.author.orcid0000-0003-0035-2439[5]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências, Baurupt
unesp.departmentCiências Biológicas - FCpt

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