Estrutura, ultraestrutura e histoquímica do aparelho digestório do Prochilodus lineatus. Análise da diversidade da microbiota intestinal de Prochilodus lineatus e Pterygoplichthys anisitsi
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2010-01-15
Autores
Makino, Lilian Cristina [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O presente trabalho teve por objetivo descrever o aparelho digestório sob os pontos de vista estrutural, ultraestrutural e histoquímico de uma espécie de peixe de água doce: o curimba (Prochilodus lineatus) (Characidae, Prochilodontidae). Adicionalmente foi realizado um estudo da diversidade da microbiota intestinal do Prochilodus lineatus e do cascudo cinza (Pterygoplichthys anisitsi). Para os estudos morfológicos de anatomia, histologia, histoquímica e de microscopia eletrônica de varredura, foram utilizados 40 curimbas adultos. Para os estudos microbiológicos, além dos curimbas, foram coletados também exemplares adultos de cascudo cinza, no qual além dos métodos de cultura e análise tradicionais, que incluíram cultivos em meios aeróbios e anaeróbios facultativos, contagem de colônias e estudo da morfologia das mesmas pelo método de Gram, também englobou técnicas de observação da microbiota em microscopia eletrônica de varredura. Os dados numéricos obtidos tanto nos trabalhos morfológicos descritivos quanto nos microbiológicos foram submetidos à análise estatística pelo programa SAS versão 9.0. O trato digestório do curimba foi dividido em intestino cefálico, intestino anterior, intestino médio e intestino posterior, os quais foram constituídos histologicamente pelas camadas mucosa, submucosa, muscular e serosa. Células caliciformes foram observadas em toda a extensão do tubo intestinal. Ao longo do tubo digestivo detectou-se a presença de mucinas neutras, ácidas ou ambas. A vesícula biliar mostrou-se como um órgão sacular alongado, oco, de fundo cego e com paredes delgadas. O fígado apresentou-se trilobado e de consistência maciça, com coloração vermelho-acastanhado, com os hepatócitos organizados em placas duplas circundadas por sinusóides. Na contagem de bactérias intestinais totais foram encontrados valores que variaram...
The aim of this study was to describe the digestive tract of species of neotropical freshwater fish, the curimba (Prochilodus lineatus) (Characidae, Prochilodontidae) under the structural, ultrastructural and histochemical aspects. Additionally a research was made about the diversity of the intestinal microflora of Prochilodus lineatus and the gray armored catfish (Pterygoplichthys anisitsi). For morphological studies, which were subdivided into gross anatomy, histology, and histochemistry and also in scanning electron microscopy were used 40 fish. For microbiological researches, were used adult specimens of the gray armored catfish, where besides of traditional methods of culture and analysis that included culture in aerobic and facultative anaerobic conditions, counting of colonies and the study of colonies morphology by Gram and also to observation of gut microbiota by scanning electron microscopy were sampled 20 fish of each species, totaling 40 animals. The numerical data obtained in the descriptive morphological researches and in the microbiological studies were analyzed by statistical software SAS, version 9.0. The digestive tract of curimba was divided into cephalic gut, foregut, midgut and hindgut, which were made histologically by the following layers: mucosal, submucosal, muscular and serosa. Goblet cells were observed throughout the length of the intestinal tube. Neutral mucins and acid or both were detected along the digestive tract. The gallbladder was an elongated saccular body, hollow, blind background and with thin walls. The liver was trilobed and compacted red-brown mass, with hepatocytes arranged in double plates surrounded by sinusoids. The count of the total intestinal bacteria showed values that ranged between 103 e 104 cfu.mL-1 (colonyforming units). The morphotypes of gram positive microorganisms found most frequently were coccoid-shaped and rod-shaped bacteria... (Complete abstract click electronic access below)
The aim of this study was to describe the digestive tract of species of neotropical freshwater fish, the curimba (Prochilodus lineatus) (Characidae, Prochilodontidae) under the structural, ultrastructural and histochemical aspects. Additionally a research was made about the diversity of the intestinal microflora of Prochilodus lineatus and the gray armored catfish (Pterygoplichthys anisitsi). For morphological studies, which were subdivided into gross anatomy, histology, and histochemistry and also in scanning electron microscopy were used 40 fish. For microbiological researches, were used adult specimens of the gray armored catfish, where besides of traditional methods of culture and analysis that included culture in aerobic and facultative anaerobic conditions, counting of colonies and the study of colonies morphology by Gram and also to observation of gut microbiota by scanning electron microscopy were sampled 20 fish of each species, totaling 40 animals. The numerical data obtained in the descriptive morphological researches and in the microbiological studies were analyzed by statistical software SAS, version 9.0. The digestive tract of curimba was divided into cephalic gut, foregut, midgut and hindgut, which were made histologically by the following layers: mucosal, submucosal, muscular and serosa. Goblet cells were observed throughout the length of the intestinal tube. Neutral mucins and acid or both were detected along the digestive tract. The gallbladder was an elongated saccular body, hollow, blind background and with thin walls. The liver was trilobed and compacted red-brown mass, with hepatocytes arranged in double plates surrounded by sinusoids. The count of the total intestinal bacteria showed values that ranged between 103 e 104 cfu.mL-1 (colonyforming units). The morphotypes of gram positive microorganisms found most frequently were coccoid-shaped and rod-shaped bacteria... (Complete abstract click electronic access below)
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Peixe de agua doce - Morfologia, Microbiologia, Morphology
Como citar
MAKINO, Lilian Cristina. Estrutura, ultraestrutura e histoquímica do aparelho digestório do Prochilodus lineatus. Análise da diversidade da microbiota intestinal de Prochilodus lineatus e Pterygoplichthys anisitsi. 2010. xvii, 92 f. Tese (doutorado) - Universidade Estadual Paulista, Centro de Aquicultura, 2010.