Suplementação alimentar de pacu (Piaractus mesopotamicus Holmberg, 1887) com β 1,3 glicano: atividade respiratória de leucócitos, lisozima e estresse por captura
Carregando...
Arquivos
Data
2007-10-26
Autores
Abreu, Janessa Sampaio de [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O sistema imune é dividido em duas partes que se complementam: o sistema imune inato (não específico) e o adquirido (específico). O sistema imune inato é considerado como a primeira linha de defesa e inclui barreiras físicas (pele e muco) e componentes celulares e moleculares (macrófagos, células killer e fatores solúveis de imunidade, como lisozima, proteínas do sistema complemento, peptídeos antimicrobianos, entre outros). No processo de criação, o sistema imune dos peixes pode ser prejudicado por vários fatores, mas efeitos benéficos podem ser observados com o uso de substâncias conhecidas como imunoestimulantes. Dentre os imunoestimulantes estudados, os glicanos vêm sendo amplamente utilizados como importantes indutores de mecanismos não específicos de defesa. Em algumas espécies de peixes tropicais, como o pacu (Piaractus mesopotamicus) e matrinxã (Brycon amazonicus), estudos observaram o papel da vitamina C como imunoestimulante avaliando a hematologia dos peixes como indicador das alterações imunológicas. O número de leucócitos e de trombócitos é considerado importante indicador de seu estado de saúde. Entretanto, não são conhecidos estudos que avaliem outros parâmetros imunológicos bem como os efeitos do glicano nas respostas imunológicas inatas de peixes tropicais. O pacu, por ser uma espécie onívora, de fácil cultivo e adaptada a ampla variedade de alimentos, tem grande importância na piscicultura brasileira e por esta razão foi escolhido como modelo experimental nesta pesquisa. O objetivo deste trabalho foi testar, inicialmente, técnicas para avaliação de alguns mecanismos inatos de defesa (atividade respiratória de leucócitos e concentração e atividade de lisozima) nesta 37 espécie tropical, cujos resultados são apresentados no capítulo 2 desta tese. Este estudo registrou essas respostas imunes inatas no pacu...
The immune system is divided in two parts that complement each other: the specific and non-specific immune system. The non-specific immune system is the primary defence line and includes natural barriers (skin and mucus) and cellular and molecular components (macrophages, natural killer cells, immune soluble factors, like lysozyme, complement, antimicrobials peptides, among others). In all farming, the immune system of fish may be damaged by many factors, but the benefic effects may be observed using immunostimulants. It has been of common knowledge that glucans are the most known of them and are being used like nonspecific defence mechanisms stimulants. In some tropical fish species, such as pacu (Piaractus mesopotamicus) and matrinxã (Brycon amazonicus), studies evaluated vitamin C effect as immunostimulant, considerating the fish hematology as immune alterations indicator. The leucocytes and trombocytes number is considerated an important health indicator. However, there are no studies evaluating different immune parameters and the glucan effect in innate immune response of tropical fish. The pacu is an omnivore specie of great importance to Brazilian fish farming. For this reason, it was chosen as experimental model in this research. The aim of this work was to test some techniques for evaluation of some defence innate mechanisms (burst oxidative and lysozyme) in this tropical specie, whose results are showed in chapter 2 of this work. This research recorded these immune responses of pacu and established adequate methodologies for its determination. After, this study evaluated the effect of glucan, administered to intraperitoneal injection and incorporated in the diet, in stimulation of some immune systems components of pacu. Besides, it was also verified 39 the stress responses after the capture of pacu fed with diets supplemented with glucan ...(Complete abstract, click electronic access below)
The immune system is divided in two parts that complement each other: the specific and non-specific immune system. The non-specific immune system is the primary defence line and includes natural barriers (skin and mucus) and cellular and molecular components (macrophages, natural killer cells, immune soluble factors, like lysozyme, complement, antimicrobials peptides, among others). In all farming, the immune system of fish may be damaged by many factors, but the benefic effects may be observed using immunostimulants. It has been of common knowledge that glucans are the most known of them and are being used like nonspecific defence mechanisms stimulants. In some tropical fish species, such as pacu (Piaractus mesopotamicus) and matrinxã (Brycon amazonicus), studies evaluated vitamin C effect as immunostimulant, considerating the fish hematology as immune alterations indicator. The leucocytes and trombocytes number is considerated an important health indicator. However, there are no studies evaluating different immune parameters and the glucan effect in innate immune response of tropical fish. The pacu is an omnivore specie of great importance to Brazilian fish farming. For this reason, it was chosen as experimental model in this research. The aim of this work was to test some techniques for evaluation of some defence innate mechanisms (burst oxidative and lysozyme) in this tropical specie, whose results are showed in chapter 2 of this work. This research recorded these immune responses of pacu and established adequate methodologies for its determination. After, this study evaluated the effect of glucan, administered to intraperitoneal injection and incorporated in the diet, in stimulation of some immune systems components of pacu. Besides, it was also verified 39 the stress responses after the capture of pacu fed with diets supplemented with glucan ...(Complete abstract, click electronic access below)
Descrição
Palavras-chave
Pacu (Peixe), Resposta imune inata, Estresse, Piaractus mesopotamicus, Glucan, Innate immune system, Stress
Como citar
ABREU, Janessa Sampaio de. Suplementação alimentar de pacu (Piaractus mesopotamicus Holmberg, 1887) com β 1,3 glicano: atividade respiratória de leucócitos, lisozima e estresse por captura. 2007. vi, 123 f. Tese (doutorado) - Universidade Estadual Paulista, Centro de Aqüicultura, 2007.