Nefropatia diabética experimental: estudo de parâmetros clínicos, laboratoriais e análise ultra-estrutural e morfométrica da membrana basal e podócitos glomerulares de ratos diabéticos induzidos pela aloxana
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Data
2004
Autores
Lerco, Mauro Masson [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Sessenta animais da espécie Rattus wistar de ambos os sexos, pesando de 200 a 300 gramas, foram divididos em dois grupos experimentais: G1 ou grupo normal, constituído por dez animais e G2 ou grupo diabético, constituído inicialmente por 50 animais, que foram inoculados com aloxana à 2% por via endovenosa, na dose de 42mg/kg de peso corporal. Desses, dez animais com diabetes experimental grave (glicemia maior que 200mg/dl), sobreviveram durante o seguimento de 12 meses e constituíram o grupo diabético ou G2. Os seguintes parâmetros clínicos e laboratoriais foram estudados em cada grupo experimental: aspecto geral dos animais, peso, ingestão hídrica, ingestão alimentar, diurese e dosagens da glicemia, glicose urinária e proteinúria. As observações, com exceção da proteinúria de 24 horas, foram observadas em três momentos experimentais: inicial ou 14 dias após a indução do diabetes no grupo diabético e 14 dias de observação no grupo normal, 6º e 12º mês de seguimento. A dosagem da proteinúria de 24 horas foi efetuada no 12º mês de seguimento nos dois grupos experimentais. Após o 12º mês de seguimento, os animais foram sacrificados, após anestesia, e o rim direito de quatro animais diabéticos e quatro animais normais, escolhidos ao acaso, foi preparado para o estudo à microscopia eletrônica. As elétron-micrografias obtidas com aumento de 42.000 vezes foram analisadas por morfometria pela utilização de sistema computadorizado Quin Lite 2,5 LEICA, interessando a espessura da MBG, o número de podócitos, o número de slit diaphragme a extensão dos slit diaphragm. Os resultados dos parâmetros clínicos e bioquímicos foram analisados pelo teste de comparações múltiplas de Tukey e pela análise não paramétrica de Mann-Whitney e Kruskal-Wallis, adotando-se o nível de significância de p< 0,05...
Sixty Rattus wistar of both sexes with 200-300 g of body weight, were divided in two experimental groups: normal group (G1 - 10 animals) and diabetic group (G2- 50 animals at the beginning), which were inoculated with a endovenous injection of 2% alloxan in a dose of 42 mg /kg/body weight. From these ten animals with severe experimental diabetics (glicemia > 200 mg%) will survive during 12 months of follow-up, being considered the experimental group (G2). The following clinic and laboratory parameters were studied: clinical aspect, body weight, water and food intake, diuresis, as well fasting glycemia, urinary glucose and proteinuria. The observations, excepting proteinuria (24 hours) were made at three experimental moments as it follows: initial or 14 days after diabetes induction or follow-up (normal controls) and at 6 and 12 months. Proteinuria was measured at the 12th month in boths groups. After 12 months of diabetes induction or follow-up (G1), the animals were sacrificed being the right kidney from four animals of each group processed for Electron Mycroscopy. The electron micrographys were taken with magnification of 42,000 and were analysed according to morphometric techniques by using computer System Quin Lite 2.5 LEICA, being studied the following aspects: glomerular basement membrane thickening, podocytes and slit diaphragm number, and the slit diaphragm extension. The results of the clinical and laboratory parameters were statistically analysed according to Tukey's multiple comparison being used the level of 5% significance. The results of morphometric study were analysed according to Mann-Whitney and Kruskal-Wallis test, using a software Sigma Stat 2,0, being used level of 5% significance. G2 group presented lower body weight, higher water intake and diuresis than G1 group; the general aspect... (Complete abstract click electronic address below)
Sixty Rattus wistar of both sexes with 200-300 g of body weight, were divided in two experimental groups: normal group (G1 - 10 animals) and diabetic group (G2- 50 animals at the beginning), which were inoculated with a endovenous injection of 2% alloxan in a dose of 42 mg /kg/body weight. From these ten animals with severe experimental diabetics (glicemia > 200 mg%) will survive during 12 months of follow-up, being considered the experimental group (G2). The following clinic and laboratory parameters were studied: clinical aspect, body weight, water and food intake, diuresis, as well fasting glycemia, urinary glucose and proteinuria. The observations, excepting proteinuria (24 hours) were made at three experimental moments as it follows: initial or 14 days after diabetes induction or follow-up (normal controls) and at 6 and 12 months. Proteinuria was measured at the 12th month in boths groups. After 12 months of diabetes induction or follow-up (G1), the animals were sacrificed being the right kidney from four animals of each group processed for Electron Mycroscopy. The electron micrographys were taken with magnification of 42,000 and were analysed according to morphometric techniques by using computer System Quin Lite 2.5 LEICA, being studied the following aspects: glomerular basement membrane thickening, podocytes and slit diaphragm number, and the slit diaphragm extension. The results of the clinical and laboratory parameters were statistically analysed according to Tukey's multiple comparison being used the level of 5% significance. The results of morphometric study were analysed according to Mann-Whitney and Kruskal-Wallis test, using a software Sigma Stat 2,0, being used level of 5% significance. G2 group presented lower body weight, higher water intake and diuresis than G1 group; the general aspect... (Complete abstract click electronic address below)
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Diabetes - Estudos experimentais, Experimental diabetics
Como citar
LERCO, Mauro Masson. Nefropatia diabética experimental: estudo de parâmetros clínicos, laboratoriais e análise ultra-estrutural e morfométrica da membrana basal e podócitos glomerulares de ratos diabéticos induzidos pela aloxana. 2004. 113 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2004.