Estudo comparativo dos efeitos da calcitonina e do plumbum metallicum 30ch na reparação óssea em mandíbula de ratos
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Data
2001-04-18
Autores
Almeida, Janete Dias [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Neste trabalho foi realizado estudo comparativo dos efeitos da calcitonina e do Plumbum mettalicum 30CH na reparação óssea guiada em mandíbulas de ratos. Foram utilizados 75 ratos com idade aproximada de três meses, que foram divididos em grupo controle, tratado com calcitonina e tratado com Plumbum mettalicum 30CH, com 25 animais cada. Todos os ratos foram submetidos à cirurgia para realização de defeito ósseo na região de ângulo de mandíbula de aproximadamente 4mm, o qual foi recoberto por uma barreira de politetrafluoretileno. Após a cirurgia, o grupo tratado com calcitonina passou a receber 2 UI/kg, i.m., três vezes por semana, e o grupo tratado com Plumbum mettalicum 30CH, três gotas diluídas em água diariamente. Os animais foram sacrificados em períodos de três, sete, 14, 21 e 28 dias, sendo suas mandíbulas removidas e encaminhadas para preparação histológica. Análises densitométrica, histológica e histomorfométrica foram realizadas. Para análise estatística, utilizou-se ANOVA, teste de Tukey e teste de Scheffé ao nível de 5%. A análise densitométrica mostrou menor densidade óptica no grupo controle em todos os períodos analisados. Foi observado o preenchimento total do defeito por tecido ósseo neoformado apenas no grupo tratado com homeopatia, aos 28 dias. Nos demais grupos não foi observada união das bordas do defeito. Na análise histomorfométrica não se encontrou diferença estatística entre os grupos tratados e controle, porém o grupo tratado com Plumbum mettalicum 30CH apresentou melhores resultados que o tratado com calcitonina. Concluiu-se que, nas condições utilizadas, a reparação óssea guiada de defeitos em mandíbulas de ratos machos é favorecida pelo Plumbum mettalicum 30CH , mas não pela calcitonina.
The purpose of this work was to compare the effects of calcitonin and Plumbum mettalicum 30CH on guided mandibular bone repair of male rats. We analyzed the mandible of 75 rats, approximately three months old, which were divided into control group, group treated with calcitonin and group treated with Plumbum mettalicum 30CH. Each group was composed by 25 animals. A circumscribed bone defect of 4mm diameter was prepared in the mandibular bone, in the angle region. The defect was covered with a PTFE barrier in all animals. The group treated with calcitonin received 2UI/Kg i.m. three times a week and the group treated with homeopathy received three drops diluted in the water every day. Both treated groups began immediately after surgery. The animals were sacrificed after three, seven, 14, 21 and 28 days. Their mandibles were removed, and after routine histological processing they were submitted to densitometric, histological and histomorphometrical analysis. For statistical analysis, ANOVA, Tukey and Scheffé tests (p<0,05) were used. The densitometric analysis showed lower optical density in the group control than in the others, at all periods. Only in the group treated with Plumbum mettalicum 30CH, the defects were completely healed at the final observation period. The histomorphometrical analysis showed no statistical difference between control, calcitonin and homeopathy treated groups, however the last one had better results than the one treated with calcitonin. It was concluded that, in circumscribed mandibular defects in male rats, Plumbum mettalicum 30CH induces better bone repair than calcitonin.
The purpose of this work was to compare the effects of calcitonin and Plumbum mettalicum 30CH on guided mandibular bone repair of male rats. We analyzed the mandible of 75 rats, approximately three months old, which were divided into control group, group treated with calcitonin and group treated with Plumbum mettalicum 30CH. Each group was composed by 25 animals. A circumscribed bone defect of 4mm diameter was prepared in the mandibular bone, in the angle region. The defect was covered with a PTFE barrier in all animals. The group treated with calcitonin received 2UI/Kg i.m. three times a week and the group treated with homeopathy received three drops diluted in the water every day. Both treated groups began immediately after surgery. The animals were sacrificed after three, seven, 14, 21 and 28 days. Their mandibles were removed, and after routine histological processing they were submitted to densitometric, histological and histomorphometrical analysis. For statistical analysis, ANOVA, Tukey and Scheffé tests (p<0,05) were used. The densitometric analysis showed lower optical density in the group control than in the others, at all periods. Only in the group treated with Plumbum mettalicum 30CH, the defects were completely healed at the final observation period. The histomorphometrical analysis showed no statistical difference between control, calcitonin and homeopathy treated groups, however the last one had better results than the one treated with calcitonin. It was concluded that, in circumscribed mandibular defects in male rats, Plumbum mettalicum 30CH induces better bone repair than calcitonin.
Descrição
Palavras-chave
Mandíbula, Ossos - Regeneração, Calcitonina, Celulas - Membranas, Regeneração óssea, Calcitonin, Histomorphometry
Como citar
ALMEIDA, Janete Dias. Estudo comparativo dos efeitos da calcitonina e do plumbum metallicum 30ch na reparação óssea em mandíbula de ratos. 2001. 129 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2001.