Avaliação da técnica de inseminação artificial intrauterina em fêmeas caninas por videolaparoscopia com sêmen fresco e descongelado
Carregando...
Arquivos
Data
2009-07-17
Autores
Alves, Aracélle Elisane [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo deste estudo foi avaliar a taxa de concepção em cadelas após inseminação artificial intrauterina por videolaparoscopia. Um total de 20 cadelas foram inseminadas, sendo 10 com sêmen fresco e 10 com sêmen congelado. Os animais se encontravam em estro natural e sob acompanhamento da citologia vaginal. Quando aproximadamente 90% das células se apresentaram cornificadas, foram realizadas dosagens séricas de progesterona afim de se determinar o momento exato da inseminação. Três machos foram utilizados como doadores de sêmen e as coletas foram realizadas por manipulação digital. Após coleta o ejaculados foram analisados quanto ao volume, motilidade, vigor e concentração espermática. Para a inseminações utilizando sêmen fresco, a coleta foi realizada momentos antes do procedimento, e o sêmen acondicionados em banho–maria a 37°C até o momento da inseminação. Amostras destinadas a inseminação com sêmen descongelado, obtiveram mesmo processo de análises após coleta, seguindo para o processo de congelação, permanecendo congeladas por no mínimo uma semana, e então descongeladas momentos antes do procedimento. De acordo com os concentrações séricas de progesterona, as inseminações intrauterinas por videolaparoscopia foram realizadas, sendo cada corno uterino inseminado com 1mL de sêmen, em concentração de 250x106 espermatozóides/mL nas cadelas inseminadas com sêmen fresco e 80x106 espermatozóides/mL naquelas inseminadas com sêmen congelado. Após 7 dias as cadelas foram ováriosalpingohisterectomizadas, sendo o lúmen das tubas e cornos uterinos lavados com solução de PBS. Embriões foram encontrados nos lavados de 7 cadelas inseminadas com sêmen fresco, e em 5 inseminadas com sêmen descongelado. Concluiu-se que o procedimento de inseminação artificial intrauterina...
The objective of this study was to evaluate the fertility in bitches after intrauterine artificial insemination by videolaparoscopy. A total of 20 bitches were inseminated, being 10 with fresh semen and 10 with frozen. The animals were in natural oestrus, and their vaginal cytology was followed. When approximately 90% of the cells were cornificated, evaluation of serum levels of progesterone was performed, with the objective to determinate the exact moment of the artificial insemination. Three males were used as donors, and the samples collection was performed by digital manipulation. After collection the ejaculates were analysed considering volume, motility, vigor and sperm concentration. For the inseminations using fresh semen the collection was performed minutes before the process, and the semen sample was maintained at 37°C. Samples destined to inseminations using frozen semen were submitted to the same analysis process after collection, followed by the freezing process, being frozen during a minimum period of one week, and thawing moments before the procedure. According to the serum levels of progesterone, the intrauterine inseminations by videolaparoscopy were performed, being each uterine corn inseminated with 1 mL of semen, with 250x106 espermatozoa/mL in bitches with fresh semen and 80x106 espermatozoa/mL in bitches with frozen semen. After 7 days the bitches were ovariohysterectomized, and the lumen of the uterine tube and corns were flushed with PBS solution. Embryos were found in 7 bitches inseminated with fresh semen, and in 5 inseminated with frozen semen. It was concluded that the intrauterine artificial insemination by videolaparoscopy seems to be an interesting reproductive biotechnology method, especially in what refers to the use of frozen semen.
The objective of this study was to evaluate the fertility in bitches after intrauterine artificial insemination by videolaparoscopy. A total of 20 bitches were inseminated, being 10 with fresh semen and 10 with frozen. The animals were in natural oestrus, and their vaginal cytology was followed. When approximately 90% of the cells were cornificated, evaluation of serum levels of progesterone was performed, with the objective to determinate the exact moment of the artificial insemination. Three males were used as donors, and the samples collection was performed by digital manipulation. After collection the ejaculates were analysed considering volume, motility, vigor and sperm concentration. For the inseminations using fresh semen the collection was performed minutes before the process, and the semen sample was maintained at 37°C. Samples destined to inseminations using frozen semen were submitted to the same analysis process after collection, followed by the freezing process, being frozen during a minimum period of one week, and thawing moments before the procedure. According to the serum levels of progesterone, the intrauterine inseminations by videolaparoscopy were performed, being each uterine corn inseminated with 1 mL of semen, with 250x106 espermatozoa/mL in bitches with fresh semen and 80x106 espermatozoa/mL in bitches with frozen semen. After 7 days the bitches were ovariohysterectomized, and the lumen of the uterine tube and corns were flushed with PBS solution. Embryos were found in 7 bitches inseminated with fresh semen, and in 5 inseminated with frozen semen. It was concluded that the intrauterine artificial insemination by videolaparoscopy seems to be an interesting reproductive biotechnology method, especially in what refers to the use of frozen semen.
Descrição
Palavras-chave
Inseminação artificial, Cadelas, Videolaparoscopia, artificial insemination, bitches, Videolaparoscopy
Como citar
ALVES, Aracélle Elisane. Avaliação da técnica de inseminação artificial intrauterina em fêmeas caninas por videolaparoscopia com sêmen fresco e descongelado. 2009. xii, 72 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2009.