Modelo experimental com caprinos e cobaias para avaliação da eficácia de vacinas contra o herpesvírus bovino tipo 1 e o vírus da diarreia viral bovina tipos 1 e 2
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Data
2012-01-16
Autores
Alexandrino, Bruna [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A presente pesquisa teve como objetivo avaliar a utilização de cobaias e caprinos para teste de vacinas contra o herpesvírus bovino tipo 1 (BoHV-1) e os vírus da diarreia viral bovina tipos 1 (BVDV-1) e 2 (BVDV-2). Inicialmente foi realizada a infecção experimental em cobaias e bovinos, com esses três vírus, para verificar a máxima resposta imunogênica em ambas as espécies. As médias geométricas dos títulos de anticorpos (GMT) dos bovinos foram altas para todos os agentes virais; em cobaias foi possível observar que o BoHV-1 induziu elevadas GMT, para o BVDV-1 a indução foi moderada, enquanto para o BVDV-2 foi baixa, podendo comprometer a viabilidade dos testes antigênicos. Após essa etapa, foram vacinados bovinos, cobaias e caprinos, com seis vacinas comerciais de antígenos inativados, para verificar a indução de anticorpos por estímulo vacinal. Nos bovinos, as GMT obtidas para o BoHV-1 mostraram que as vacinas utilizadas promoveram a indução moderada na resposta imunológica, e apenas três delas foram consideradas satisfatórias. Para o BVDV-1, apenas uma vacina pode ser considerada eficiente, e em relação ao BVDV-2 nenhuma delas poderia ser assim classificada. Os resultados da vacinação com os caprinos mostraram que, apesar de os animais serem reagentes ao BoHV-1 no início do experimento, as formulações foram capazes de induzir a produção de anticorpos com títulos elevados contra os três vírus estudados. As cobaias, por sua vez, receberam doses fracionadas de uma vacina comercial nacional e, das frações testadas, 3,2mL foi a que apresentou melhores resultados, sendo esta estabelecida para testar as demais vacinas nesta espécie. Depois da imunização, os resultados mostram que para o BoHV-1 as GMT foram altas; em relação ao BVDV-1, apenas duas vacinas foram capazes de induzir...
The present research had as objective to evaluate the use of guinea pigs and goats in test of vaccines against the bovine herpesvirus type 1 (BoHV-1) and the bovine viral diarrhea viruses types 1 (BVDV-1) and 2 (BVDV-2). First, an experimental infection was realized in guinea pigs and bovines, using these three viruses, to verify the maximum immune response in both species. The geometric means of antibodies titres (GMT) for bovines were high for the viral agents; in guinea pigs was possible to observe that the GMT induced by BoHV-1 was high, by BVDV-1 was moderate and by BVDV-2 was weak and could compromise the viability of antigenic tests. After this stage, the bovines, guinea pigs and goats were vaccinated using six commercial vaccines with inactivated antigens to verify the induction of antibodies production by vaccinal stimulus. In bovines, the GMT obtained for BoHV-1 showed the vaccines induced a moderate immune response, being only three of them considered satisfactory. For BVDV-1 only one and for BVDV-2 none of them can have the same classification. The results obtained with the goats, despite they were positive to BoHV-1 in the sorting, showed the formulations were able to induce high antibody production against the three virus studied. The guinea pigs, on the other hand, received fractional doses of a commercial vaccine well-established in the domestic trade and, from the fractions tested, 3.2 mL showed the best results, being established to test the other vaccines. After immunization, the results of GMT for BoHV-1 was high; relating to BVDV-1, only two vaccines were capable to induce antibodies production, but their GMT were weak; and there was no immune response against BVDV-2. Thus, goats can be ... (Complete abstract click electronic access below)
The present research had as objective to evaluate the use of guinea pigs and goats in test of vaccines against the bovine herpesvirus type 1 (BoHV-1) and the bovine viral diarrhea viruses types 1 (BVDV-1) and 2 (BVDV-2). First, an experimental infection was realized in guinea pigs and bovines, using these three viruses, to verify the maximum immune response in both species. The geometric means of antibodies titres (GMT) for bovines were high for the viral agents; in guinea pigs was possible to observe that the GMT induced by BoHV-1 was high, by BVDV-1 was moderate and by BVDV-2 was weak and could compromise the viability of antigenic tests. After this stage, the bovines, guinea pigs and goats were vaccinated using six commercial vaccines with inactivated antigens to verify the induction of antibodies production by vaccinal stimulus. In bovines, the GMT obtained for BoHV-1 showed the vaccines induced a moderate immune response, being only three of them considered satisfactory. For BVDV-1 only one and for BVDV-2 none of them can have the same classification. The results obtained with the goats, despite they were positive to BoHV-1 in the sorting, showed the formulations were able to induce high antibody production against the three virus studied. The guinea pigs, on the other hand, received fractional doses of a commercial vaccine well-established in the domestic trade and, from the fractions tested, 3.2 mL showed the best results, being established to test the other vaccines. After immunization, the results of GMT for BoHV-1 was high; relating to BVDV-1, only two vaccines were capable to induce antibodies production, but their GMT were weak; and there was no immune response against BVDV-2. Thus, goats can be ... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Herpes vírus bovino - Vacina, Vacinas virais, Bovine herpesvirus - Vaccine
Como citar
ALEXANDRINO, Bruna. Modelo experimental com caprinos e cobaias para avaliação da eficácia de vacinas contra o herpesvírus bovino tipo 1 e o vírus da diarreia viral bovina tipos 1 e 2. 2012. xvi, 81 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2012.