Comparação filogenética de genomas de rizóbios por hibridização através de microarray
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Data
2007-12-14
Autores
Pereira, Rodrigo Matheus [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Diversos estudos com bactérias fixadoras de nitrogênio buscam identificar genes responsáveis pela fixação de nitrogênio, nodulação e simbiose, assim como conhecer seu genoma e compreender melhor o metabolismo delas. No entanto ainda há poucos trabalhos sobre o genoma de Bradyrhizobium elkanii, bactéria importante no contexto da produção de alimentos e de utilização comercial. Esse é o primeiro estudo a comparar 2654 genes de Bradyrhizobium elkanii SEMIA 587, com as bactérias Bradyrhizobium japonicum LGM 6138, Azorhizobium caulinodans LGM 6465, Mesorhizobium huakuii LGM 14107, Rhizobium leguminosarum bv. Trifolii LGM 8820 e Sinorhizobium meliloti LGM 6133 através da comparação de genomas por hibridização (CGH) usando Microarray. Portanto o objetivo deste trabalho foi realizar uma taxonomia molecular através da comparação de genomas por hibridização DNA-DNA, utilizando a tecnologia de microarranjos de DNA (CGH Microarray), para buscar novos genes que possam ser úteis na classificação filogenética de rizóbios. A técnica de CGH Microarray permitiu encontrar sessenta e cinco genes comuns entre todas as bactérias analisadas, após realizar comparação e classificação de todos genes encontrados em comum entre elas. Os resultados obtidos ao se comparar três árvores filogenéticas baseadas em diferentes genes, confirmaram que alterar a quantidade e as sequências nas análises, causa variação nas árvores filogenéticas como já observado em outros microrganismos...
Diverse studies with fixing nitrogen bacteria search to identify responsible genes for the nitrogen fix, nodulation and symbiosis, as well as knowing its genome and understanding the metabolism of them better. However still has few works about genome of Bradyrhizobium elkanii, important bacterium in the context of the production of foods and commercial use. This is the first study to compare 2654 genes of Bradyrhizobium elkanii SEMIA 587, with the bacteria Bradyrhizobium japonicum LGM 6138, Azorhizobium caulinodans LGM 6465, Mesorhizobium huakuii LGM 14107, Rhizobium leguminosarum bv. Trifolii LGM 8820 and Sinorhizobium meliloti LGM 6133 through the CGH Microarray. Therefore aim of this work was to carry a molecular taxonomy through the comparison of genomes for hybridization DNA-DNA, being used the technology of microarrangements of DNA (CGH Microarray), to search new genes that can be useful in the phylogenetic classification of rhizobia. The technique of CGH Microarray allowed to find sixty and five orthologs genes between all the analyzed bacteria, after to carry through comparison and classification of all genes in common between them. The results gotten to if comparing three established phylogenetic trees in different genes, had confirmed that to modify the amount and the sequences in the analyses, cause variation in the phylogenetic trees as already observed in other microorganisms. The results suggest that how much bigger will be the number of used ortholog genes in the phylogeny, more trustworthy will be the gotten phylogenetic trees ...(Complete abstract, click electronic access below)
Diverse studies with fixing nitrogen bacteria search to identify responsible genes for the nitrogen fix, nodulation and symbiosis, as well as knowing its genome and understanding the metabolism of them better. However still has few works about genome of Bradyrhizobium elkanii, important bacterium in the context of the production of foods and commercial use. This is the first study to compare 2654 genes of Bradyrhizobium elkanii SEMIA 587, with the bacteria Bradyrhizobium japonicum LGM 6138, Azorhizobium caulinodans LGM 6465, Mesorhizobium huakuii LGM 14107, Rhizobium leguminosarum bv. Trifolii LGM 8820 and Sinorhizobium meliloti LGM 6133 through the CGH Microarray. Therefore aim of this work was to carry a molecular taxonomy through the comparison of genomes for hybridization DNA-DNA, being used the technology of microarrangements of DNA (CGH Microarray), to search new genes that can be useful in the phylogenetic classification of rhizobia. The technique of CGH Microarray allowed to find sixty and five orthologs genes between all the analyzed bacteria, after to carry through comparison and classification of all genes in common between them. The results gotten to if comparing three established phylogenetic trees in different genes, had confirmed that to modify the amount and the sequences in the analyses, cause variation in the phylogenetic trees as already observed in other microorganisms. The results suggest that how much bigger will be the number of used ortholog genes in the phylogeny, more trustworthy will be the gotten phylogenetic trees ...(Complete abstract, click electronic access below)
Descrição
Palavras-chave
Rizobio, Filogenia, Genes ortólogos, Bradyrhizobium elkanii, Ortholog genes, Phylogeny
Como citar
PEREIRA, Rodrigo Matheus. Comparação filogenética de genomas de rizóbios por hibridização através de microarray. 2007. x, 87 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2007.