Resposta tecidual de dentes reimplantados utilizando plasma pobre em plaquetas, plasma rico em plaquetas e células-tronco da medula óssea: estudo em cães
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Data
2009-07-31
Autores
Assunção, Luciana Reichert da Silva [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A avulsão dentária é um tipo de trauma que resulta na perda da continuidade de um complexo e múltiplo compartimento de tecidos. Componentes derivados do sangue, incluindo o plasma pobre em plaquetas (PPP) e o plasma rico em plaquetas (PRP) constituem uma opção terapêutica para o reparo tecidual. O gel de PRP tem sido usado como arcabouço para células-tronco da medula óssea em Engenharia Tecidual. O propósito da presente investigação foi avaliar o efeito do PPP, PRP ativado ou não por trombina e células-tronco (CT) originárias da medula óssea associadas ao PRP no reparo de reimplantes dentários. Quatro cães foram utilizados neste estudo. PRP e PPP foram obtidos pelo método de dupla centrifugação a partir do sangue coletado da veia jugular dos animais. CT foram obtidas por aspiração da medula óssea (AMO) e isoladas pelo método de centrifugação por densidade de gradiente. A citometria de fluxo (CF) foi realizada após AMO utilizando marcadores celulares CD34 e CD90. Após 30 minutos da extração, os dentes foram reimplantados e, de acordo com a proposta metodológica, divididos em 5 grupos: Grupo I – dentes reimplantados sem material (Controle); Grupo II – dentes reimplantados com o Plasma Pobre em Plaquetas (PPP); Grupo III – dentes reimplantados com o Plasma Rico em Plaquetas ativado por cloreto de cálcio (PRP); Grupo IV – dentes reimplantados com o Plasma Rico em Plaquetas ativado por trombina de origem bovina e cloreto de cálcio (PRP + TR) e Grupo V – dentes reimplantados com células-tronco da medula óssea associadas ao PRP ativado por cloreto de cálcio (CT + PRP). Análises histológica, histomorfométrica e imuno histoquímica foram realizadas 120 dias após o reimplante. Análise de variância (ANOVA) e o teste post-hoc de Tukey foram utilizados para a análise estatística, com nível de significância de 5%. CF mostrou...
Dental avulsion constitutes a traumatism which results in the loss of a complex and multiple tissue compartments. Blood-components products, including platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute a therapeutic option for tissue healing. PRP gel has also been used as a scaffold for bone marrow stem cells in Tissue Engineering. This study aimed to evaluate the effect of PPP, PRP activated or not with thrombin and bone marrow stem cells (BMSC) associated with PRP on the healing of replanted teeth. Four adult hybrid dogs were used in this study. PRP and PPP were obtained trough double centrifugation technique of blood collected from the jugular vein of the animals. CTM were obtained from bone marrow aspiration (BMA) and isolated by density gradient centrifugation method. Flow Cytometry Analysis (FCA) was performed after BMA using CD34 and CD90 cells markers. After 30 minutes of extraction, teeth were replanted and, according to the methodological proposal, divided into 5 Groups: Group I – teeth replanted without any material (Control); Group II – teeth replanted with Platelet-poor Plasma (PPP); Group III – teeth replanted with Platelet-rich plasma activated by sodium chloride (PRP); Group IV – teeth replanted with Platelet-rich plasma activated by bovine thrombin and sodium chloride (PRP + TR) and Group V – teeth replanted with bone marrow stem cells associated with PRP activated by sodium chloride (BMSC + PRP). Histological, histomorphometric and immunohistochemical analysis were assessed 120 days after reimplantation. Analysis of variance (ANOVA) and Tukey post-hoc test were used for the statistical analysis, with the level of significance set at 5%. FCA showed 55.98% of CD34+ and 33.28% of CD90+ after BMA. Group V (BMSC + PRP) presented the largest areas of replacement resorption, confirmed... (Complete abstract click electronic access below)
Dental avulsion constitutes a traumatism which results in the loss of a complex and multiple tissue compartments. Blood-components products, including platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute a therapeutic option for tissue healing. PRP gel has also been used as a scaffold for bone marrow stem cells in Tissue Engineering. This study aimed to evaluate the effect of PPP, PRP activated or not with thrombin and bone marrow stem cells (BMSC) associated with PRP on the healing of replanted teeth. Four adult hybrid dogs were used in this study. PRP and PPP were obtained trough double centrifugation technique of blood collected from the jugular vein of the animals. CTM were obtained from bone marrow aspiration (BMA) and isolated by density gradient centrifugation method. Flow Cytometry Analysis (FCA) was performed after BMA using CD34 and CD90 cells markers. After 30 minutes of extraction, teeth were replanted and, according to the methodological proposal, divided into 5 Groups: Group I – teeth replanted without any material (Control); Group II – teeth replanted with Platelet-poor Plasma (PPP); Group III – teeth replanted with Platelet-rich plasma activated by sodium chloride (PRP); Group IV – teeth replanted with Platelet-rich plasma activated by bovine thrombin and sodium chloride (PRP + TR) and Group V – teeth replanted with bone marrow stem cells associated with PRP activated by sodium chloride (BMSC + PRP). Histological, histomorphometric and immunohistochemical analysis were assessed 120 days after reimplantation. Analysis of variance (ANOVA) and Tukey post-hoc test were used for the statistical analysis, with the level of significance set at 5%. FCA showed 55.98% of CD34+ and 33.28% of CD90+ after BMA. Group V (BMSC + PRP) presented the largest areas of replacement resorption, confirmed... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Medula ossea, Células-Tronco, Reimplante dentário, Plasma rico em plaquetas, Tooth replantation, Platelet-rich plasma, Stem cells, Bone marrow
Como citar
ASSUNÇÃO, Luciana Reichert da Silva. Resposta tecidual de dentes reimplantados utilizando plasma pobre em plaquetas, plasma rico em plaquetas e células-tronco da medula óssea: estudo em cães. 2009. 248 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2009.