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dc.contributor.advisorViero, Rosa Marlene [UNESP]
dc.contributor.advisorSartori, Alexandrina [UNESP]
dc.contributor.authorDomingues, Alexandre [UNESP]
dc.date.accessioned2014-06-11T19:33:24Z
dc.date.available2014-06-11T19:33:24Z
dc.date.issued2011-02-25
dc.identifier.citationDOMINGUES, Alexandre. Avaliação da segurança e da atividade imunomoduladora dos extratos de Uncaria tomenosa e Uncaria guianensis sobre a patogenia dos Diabetes Melito autoimune induzido pela estreptozotocina. 2011. 159 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2011.
dc.identifier.urihttp://hdl.handle.net/11449/104553
dc.description.abstractUncaria tomentosa (Willd.) DC (Rubiaceae) is a large woody vine that is native to the Amazon and Central American rainforests and is widely used in traditional medicine for its immunomodulatory and anti-inflammatory activities. The present work used in vivo immunotoxic and in vitro immunomodulatory experiments to investigate the effects of a pentacyclic oxindole alkaloid extract from U. tomentosa bark on lymphocyte phenotype, Th1/Th2 cytokine production, cellular proliferation and cytotoxicity. For the in vivo immunotoxicity testing, BALB/c male mice were treated once a day with 125, 500 or 1250 mg/kg of U. tomentosa extract for 28 days. For the in vitro protocol, lymphocytes were cultured with 10 to 500 μL/mL of the extract for 48 h. The extract increased the cellularity of splenic white pulp and the thymic medulla and increased the number of T helper lymphocytes and B lymphocytes. Also, a large stimulatory effect on lymphocyte viability was observed. However, mitogen-induced T lymphocyte proliferation was significantly inhibited at higher concentrations of U. tomentosa extract. Furthermore, an immunological polarization toward a Th2 cytokine profile was observed. These results suggest that the U. tomentosa aqueous-ethanol extract was not immunotoxic to mice and was able to modulate distinct patterns of the immune system in a dose-dependent manneren
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent159 f.
dc.language.isopor
dc.publisherUniversidade Estadual Paulista (UNESP)
dc.sourceAleph
dc.subjectDiabetes - Aspestos imunológicospt
dc.subjectUncaria tomentosaen
dc.subjectImmune systemen
dc.titleAvaliação da segurança e da atividade imunomoduladora dos extratos de Uncaria tomenosa e Uncaria guianensis sobre a patogenia dos Diabetes Melito autoimune induzido pela estreptozotocinapt
dc.typeTese de doutorado
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.rights.accessRightsAcesso aberto
unesp.graduateProgramPatologia - FMBpt
unesp.knowledgeAreaPatologiapt
unesp.researchAreaImunomodulação/Imunotoxicologiapt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
dc.identifier.aleph000638839
dc.identifier.filedomingues_a_dr_botfm.pdf
dc.identifier.capes33004064056P5
dc.identifier.lattes1453564171333848
unesp.author.lattes1453564171333848
unesp.advisor.lattes4977572416129527[2]
unesp.advisor.orcid0000-0003-4557-3331[2]
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