Resposta celular no encéfalo de coelhos experimentalmente inoculados com herpesvírus bovino 5 (BoHV-5)
Carregando...
Data
2012-01-30
Autores
Pedraza-Ordóñez, Francisco Javier [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O BoHV-5 é um alfa herpesvírus neurovirulento, que causa meningoencefalite fatal em bezerros. A morbidade é baixa contrastando com uma alta mortalidade, embora alguns animais possam-se recuperar. Varias pesquisas tem sido desenvolvidas para determinar a patogênese deste tipo de doença. No presente trabalho coelhos experimentalmente infectados com o Herpesvírus Bovino 5 (BoHV-5) foram submetidos a análise imuno-histoquímica e molecular, mediante a real time PCR, para descrever a resposta inflamatória em seis diferentes regiões do seu encéfalo. Todos os animais mostraram sinais neurológicos severos e foram eutanasiados vinte dias após a infecção inicial. Microscopicamente foi descrita uma meningoencefalite não supurativa, caracterizada por meningite, manguitos perivasculares mononucleares e malacia, mas não foram observados corpúsculos de inclusão viral. Os linfócitos T constituíram uma alta percentagem das células mononucleares envolvidas na neuroinflamação. Não foram encontradas diferenças significativas na resposta astrocitária quando comparados o grupo experimental e o grupo controle (p > 0,05). A quantificação viral pela qPCR permitiu achar partículas virais em todas as regiões encefálicas, de todos os animais do grupo experimental, sendo diretamente proporcional a quantidade de vírus e a visualização de lesões histológicas no encéfalo dos coelhos, assim como a imunodetecção do BoHV-5 pela imunohistoquímica. É possível suspeitar de um forte envolvimento do sistema imunitário do hospedeiro que fez a maioria dos animais reagir de maneira diferente ante a mesma quantidade do inóculo viral ministrado
The BoHV-5 is a neurovirulent alpha herpesvirus, which causes fatal meningoencephalitis in calves. The morbidity is low in contrast with a high mortality, although some animals can heal. Several surveys have been developed trying to better understand the pathogenesis of this type of disease. In this study rabbits experimentally infected with bovine herpesvirus 5 (BoHV-5) were subjected to immunohistochemical and molecular analysis by real time PCR, to describe the inflammatory response in six different regions of his brain. All animals showed severe neurological signs and were euthanized twenty days after the initial infection. Microscopically was described a meningoencephalitis characterized by nonsuppurative meningitis, mononuclear perivascular cuffs and malacia, but were not observed viral inclusion corpuscles. T lymphocytes constituted a high percentage of mononuclear cells involved in neuroinflammation and there were no significant differences in astrocyte response when comparing the experimental and control groups (p> 0.05). The viral quantification by qPCR allowed found viral particles in all brain regions of all experimental animals, being directly proportional to the amount of virus and the visualization of lesions in the brain of rabbits, as well as immunodetection of BoHV-5 by immunohistochemistry. It is possible to suspect a strong engagement of the host immune system that made the majority of animals respond differently compared to the same administered amount of viral inoculum
The BoHV-5 is a neurovirulent alpha herpesvirus, which causes fatal meningoencephalitis in calves. The morbidity is low in contrast with a high mortality, although some animals can heal. Several surveys have been developed trying to better understand the pathogenesis of this type of disease. In this study rabbits experimentally infected with bovine herpesvirus 5 (BoHV-5) were subjected to immunohistochemical and molecular analysis by real time PCR, to describe the inflammatory response in six different regions of his brain. All animals showed severe neurological signs and were euthanized twenty days after the initial infection. Microscopically was described a meningoencephalitis characterized by nonsuppurative meningitis, mononuclear perivascular cuffs and malacia, but were not observed viral inclusion corpuscles. T lymphocytes constituted a high percentage of mononuclear cells involved in neuroinflammation and there were no significant differences in astrocyte response when comparing the experimental and control groups (p> 0.05). The viral quantification by qPCR allowed found viral particles in all brain regions of all experimental animals, being directly proportional to the amount of virus and the visualization of lesions in the brain of rabbits, as well as immunodetection of BoHV-5 by immunohistochemistry. It is possible to suspect a strong engagement of the host immune system that made the majority of animals respond differently compared to the same administered amount of viral inoculum
Descrição
Palavras-chave
Encefalite, Virus do herpes em animais, Herpesvirus infection, Viral encephalitis
Como citar
PEDRAZA-ORDÓÑEZ, Francisco Javier. Resposta celular no encéfalo de coelhos experimentalmente inoculados com herpesvírus bovino 5 (BoHV-5). 2012. xiv, 59 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2012.