Resveratrol na apoptose e produção de superóxido em neutrófilos de cães
Data
2012-12-05
Autores
Castro, Marinês de [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A destruição dos micro-organismos ocorre principalmente por mecanismos dependentes de oxigênio e pela liberação de enzimas líticas dos grânulos intracelulares dos neutrófilos. Neutrófilos ativados in vitro e in vivo geram espécies reativas de oxigênio e sofrem apoptose rapidamente. O resveratrol possui efeitos protetores na aterosclerose, possui potencial anticancerígeno e antiinflamatório podendo também atuar como protetor e regenerador dos antioxidantes primários do organismo. O objetivo do presente estudo foi verificar o efeito protetor de diferentes concentrações de resveratrol na apoptose e produção de superóxido de neutrófilos. Uma suspensão de neutrófilos final de 1 x 106 mL de 10 cães adultos hígidos foram aliquotadas e pré- incubadas com diferentes concentrações finais de resveratrol (0, 30, 60 e 90 Rmol) durante 4 horas. A taxa de apoptose foi determinada por citometria de fluxo capilar, utilizando-se do sistema Anexina V-PE e a de superoxido por sonda HE. Nos grupos tratados com resveratrol (30, 60 e 90 Rmol) e estimulados com CAM (camptotecina) não houve diminuição nos índices apoptóticos. Na presença de PMA (forbol-12-miristato 13 acetato) a fluorescência média do brometo foi superior em todos os tratamentos. Nos grupos tratados com RV e estimulados com PMA não houve diminuição na quantidade de superóxido. Conclusões: Nas doses utilizadas o resveratrol não inibiu a apoptose de neutrófilos. A incubação dos neutrófilos com RV não afetou a viabilidade celular. As taxas de produção de superóxido não diferiram entre os tratamentos utilizados p>0,05, constatado através de citometria de fluxo
The destruction of microorganisms occurs mainly by oxygendependent mechanisms involving the generation of reactive oxygen metabolites and also the liberation of lytic enzymes from intracellular granules. Activated neutrophils generate ROS and suffer apoptosis quickly, verified in-vitro and in- vivo. Resveratrol has protective effects against atherosclerosis, it has anticarcinogenic and anti inflammatory potential and it may also protect and regenerate body’s primary antioxidants. The objective of the present study was to examine the possible protective effect of different concentrations of resveratrol on apoptosis and superoxide production of stimulated neutrophils. A final neutrophil suspension of 1 x 106 of 10 adult healthy dogs was aliquoted and pre-incubated with different final concentrations of resveratrol (0, 30, 60 e 90 Rmol) for 4 hours. Superoxide production rate and apoptosis was determined by capillary flow cytometry used anexin V-PE system and HE probe. In PMA(forbol-12-miristato 13 acetato) presence, bromide average fluorescence was superior in every treatment. In the groups treated with resveratrol and stimulated with PMA there was no reductions in amount of superoxide. In groups treated with resveratrol and stimulated with CAM (camptotecina) there was no decrease in apoptotic index. It was also observed that the concentrations of RV used did not affected cellular viability
The destruction of microorganisms occurs mainly by oxygendependent mechanisms involving the generation of reactive oxygen metabolites and also the liberation of lytic enzymes from intracellular granules. Activated neutrophils generate ROS and suffer apoptosis quickly, verified in-vitro and in- vivo. Resveratrol has protective effects against atherosclerosis, it has anticarcinogenic and anti inflammatory potential and it may also protect and regenerate body’s primary antioxidants. The objective of the present study was to examine the possible protective effect of different concentrations of resveratrol on apoptosis and superoxide production of stimulated neutrophils. A final neutrophil suspension of 1 x 106 of 10 adult healthy dogs was aliquoted and pre-incubated with different final concentrations of resveratrol (0, 30, 60 e 90 Rmol) for 4 hours. Superoxide production rate and apoptosis was determined by capillary flow cytometry used anexin V-PE system and HE probe. In PMA(forbol-12-miristato 13 acetato) presence, bromide average fluorescence was superior in every treatment. In the groups treated with resveratrol and stimulated with PMA there was no reductions in amount of superoxide. In groups treated with resveratrol and stimulated with CAM (camptotecina) there was no decrease in apoptotic index. It was also observed that the concentrations of RV used did not affected cellular viability
Descrição
Palavras-chave
Cão, Antioxidantes, Citometria de fluxo, Neutrófilos, Apoptose, Superoxido, Antioxidants
Como citar
CASTRO, Marinês de. Resveratrol na apoptose e produção de superóxido em neutrófilos de cães. 2012. vi, 57 p. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2012.