Efeito da adição e/ou suplementação de antioxidante no processo de congelação/descongelação de sêmen de cães férteis e subférteis
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Data
2010-08-27
Autores
Lopes, Bethania Vieira [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo desse projeto foi determinar a eficiência do uso de antioxidantes na prevenção de efeitos deletérios da congelação de sêmen em cães férteis e subférteis. Para isso foram executados dois experimentos: O primeiro teve como objetivo comparar os resultados da congelação do sêmen de cães férteis e subférteis, utilizando meio diluente a base de TRIS/gema de ovo/glicerol, com a adição ou não de antioxidantes (Catalase, Superóxido Dismutase e suas associações, ácido ascórbico, tocoferol e suas associações). O segundo experimento teve como objetivo comparar os resultados da congelação do sêmen de cães férteis e subférteis, suplementados com 500mg de vitamina C e E, por via oral, durante 60 dias. Foram utilizados 13 cães, sendo 7 considerados férteis e 6 classificados com subférteis. Os cães férteis apresentavam exame andrológico dentro dos padrões recomendados pelo CBRA; os subférteis apresentavam baixa concentração espermática (< 20x10 6 sptz/mL) e/ ou alto índice de patologias espermáticas (> 30%). O sêmen foi congelado em três momentos, antes do início da suplementação (M1), 30 (M2) e 60 dias (M3) após o início da suplementação oral. O sêmen foi coletado por manipulação digital do pênis e analisado para: motilidade, vigor, concentração e morfologia espermática. A congelação do sêmen foi realizada pelo método descrito por Chirinéa et al. (2006). A descongelação foi realizada a 70ºC por 8 segundos. Logo após as amostras foram avaliadas na análise computadorizada (CASA) e avaliação de integridade de membrana acrossomal e plasmática. Também foi analisada a taxa de peroxidação lipídica e antioxidantes: SOD, catalase, vitaminas C e E no plasma seminal. Pode-se concluir que a suplementação oral, no meio diluente ou a sua associação pode trazer benefícios para o processo de congelação/descongelação de cães...
The aim of this project was to determine the efficiency of antioxidants in preventing the deleterious effects of freezing semen in fertile and subfertile dogs. For this purpose two experiments were performed: The first aim was to compare the results of frozen semen of fertile and subfertile dogs, using the TRIS diluent / egg yolk / glycerol extender, with or without addition of antioxidants (Catalase, Superoxide dismutase and their associations, ascorbic acid, tocopherol and their associations). The second experiment aimed to compare the results of frozen semen of fertile and subfertile dogs supplemented with 500mg of vitamin C and E, orally for 60 days. We used 13 dogs, 7 were considered fertile and 6 classified as subfertile. The dogs had been considered fertile breeding soundness examination within the standards recommended by CBRA; the subfertile had lower sperm concentration (<20x10 6 sptz / mL) and / or high rate of sperm pathologies (> 30%). Semen was frozen in three stages, before the start of supplementation (M1), 30 (M2) and 60 days (M3) after the initiation of supplementation. Semen was collected by digital manipulation of the penis and analyzed for: motility, vigor, concentration and morphology. The frozen semen was performed by the method described by Chirinea et al. (2006). Thawing was performed at 70 ° C for 8 seconds. Soon after the samples were evaluated in the computerized analysis (CASA) and assessment of membrane integrity and acrosomal plasma. We also analyzed the rate of lipid peroxidation and antioxidants: SOD, catalase, vitamins C and E in seminal plasma. It can be conclude that oral supplementation, in the extender or your association can benefit the process of freezing / thawing of dogs. Especially for those with reproductive problems and may even, with oral therapy to reduce or eliminate this feature
The aim of this project was to determine the efficiency of antioxidants in preventing the deleterious effects of freezing semen in fertile and subfertile dogs. For this purpose two experiments were performed: The first aim was to compare the results of frozen semen of fertile and subfertile dogs, using the TRIS diluent / egg yolk / glycerol extender, with or without addition of antioxidants (Catalase, Superoxide dismutase and their associations, ascorbic acid, tocopherol and their associations). The second experiment aimed to compare the results of frozen semen of fertile and subfertile dogs supplemented with 500mg of vitamin C and E, orally for 60 days. We used 13 dogs, 7 were considered fertile and 6 classified as subfertile. The dogs had been considered fertile breeding soundness examination within the standards recommended by CBRA; the subfertile had lower sperm concentration (<20x10 6 sptz / mL) and / or high rate of sperm pathologies (> 30%). Semen was frozen in three stages, before the start of supplementation (M1), 30 (M2) and 60 days (M3) after the initiation of supplementation. Semen was collected by digital manipulation of the penis and analyzed for: motility, vigor, concentration and morphology. The frozen semen was performed by the method described by Chirinea et al. (2006). Thawing was performed at 70 ° C for 8 seconds. Soon after the samples were evaluated in the computerized analysis (CASA) and assessment of membrane integrity and acrosomal plasma. We also analyzed the rate of lipid peroxidation and antioxidants: SOD, catalase, vitamins C and E in seminal plasma. It can be conclude that oral supplementation, in the extender or your association can benefit the process of freezing / thawing of dogs. Especially for those with reproductive problems and may even, with oral therapy to reduce or eliminate this feature
Descrição
Palavras-chave
Reprodução animal, Semen - Criopreservação, Cão, Antioxidants
Como citar
LOPES, Bethania Vieira. Efeito da adição e/ou suplementação de antioxidante no processo de congelação/descongelação de sêmen de cães férteis e subférteis. 2010. 102 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinaria e Zootecnia, 2010.