Efeito da córtico-terapia e plasma seminal na resposta inflamatória e fertilidade de éguas submetidas à inseminação arterial
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Data
2004
Autores
Dell'aqua Junior, José Antônio [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Com o intuito de verificar a ação do acetato de prednisolona e do plasma seminal sobre a resposta inflamatória uterina de éguas inseminadas com sêmen congelado, desenvolveu-se os seguintes procedimentos: inicialmente 36 éguas foram aleatoriamente divididas em 6 grupos experimentais: G1 grupo controle, éguas não inseminadas; G2 éguas inseminadas com 800 x 106 de espermatozóides in natura; G3 éguas inseminadas com sêmen congelado 800 x 106 de espermatozóides viáveis pré-congelação; G4 éguas inseminadas com sêmen congelado 800 x 106 de espermatozóides viáveis pré-congelação + adição de 20 mL de plasma seminal; G5 éguas inseminadas com sêmen congelado 800 x 106 de espermatozóides viáveis pré-congelação + corticóide-terapia; G6 éguas inseminadas com sêmen congelado 800 x 106 de espermatozóides viáveis pré-congelação + adição de 20 mL de plasma seminal + corticóide-terapia. A corticóide-terapia foi iniciada quando os animais apresentavam folículos de 35 mm e edema uterino, neste momento, se efetuava a administração da gonadotrofina coriônica humana (hCG) e concomitantemente 0,1 mg/kg de acetato de prednisolona por via intramuscular (Predef ), e repetia-se o corticóide a cada 12 horas até a ovulação. Os animais dos grupos tratados com corticóide G5 e G6, demonstraram um decréscimo significativo na função neutrofílica, frente ao teste da redução do tetrazólio nitroazul (NBT). O plasma seminal não alterou a intensidade da resposta inflamatória em nenhum dos testes realizados. No teste de fertilidade foram realizados dois grupos de inseminação, no primeiro, foram utilizadas éguas sem histórico de resposta inflamatória intensa pós-inseminação e não foi vista diferença estatística nos resultados de prenhes. Entretanto, no segundo teste de fertilidade, onde foram usadas éguas problemas, com... .
Aiming to verify the action of prednizolone acetate and of seminal plasma on the uterine inflammatory response of mares inseminated with frozen semen, the following procedures were developed. Thirty six mares were randomly allocated in 6 experimental groups: G1 control groups, with non-inseminated mares; G2 mares inseminated with 800 x 106 in natura sperm cells; G3 mares inseminated with 800 x 106 frozen-thawed sperm cells; G4 mares inseminated with 800 x 106 frozen-thawed sperm cells + 20 mL seminal plasma; G5 mares inseminated with 800 x 106 frozen-thawed sperm cells + cortico-therapy; G6 mares inseminated with 800 x 106 frozen-thawed sperm cells + 20 mL seminal plasma + cortico-therapy. The therapy with 0,1 mg/kg of prednizolone acetate (IM) (Predef ), was performed when the mares presented follicles with 35 mm and uterine edema. At this moment the ovulation was induced with human corionic gonadotrophin (hCG), and the corticoid administrations repeated every 12 hours until ovulation. In G5 and G6 when the animals were treated with corticoid, it was observed a significant decrease on the neutrophil function, analyzed by the tetrazolio nitroazul test (NBT). The infusion of seminal plasma did not modify the uterine inflammatory response in any of the performed tests. For the fertility test, two groups of inseminated mares were used. On the first one, mares with no history of intense uterine inflammatory response after insemination were used. No statistic differences were observed on pregnancy results. However, on the second group of fertility test when problem mares, with history of post-insemination endometritis, were used, the corticoid-treated group presented a significant high pregnancy rate when compared with the control group (67% and 0% respectively). The cortico-therapy showed to be a safe method with no side effects on treated animals, representing an adequate choice for animals... (Complete abstract, click electronic address below).
Aiming to verify the action of prednizolone acetate and of seminal plasma on the uterine inflammatory response of mares inseminated with frozen semen, the following procedures were developed. Thirty six mares were randomly allocated in 6 experimental groups: G1 control groups, with non-inseminated mares; G2 mares inseminated with 800 x 106 in natura sperm cells; G3 mares inseminated with 800 x 106 frozen-thawed sperm cells; G4 mares inseminated with 800 x 106 frozen-thawed sperm cells + 20 mL seminal plasma; G5 mares inseminated with 800 x 106 frozen-thawed sperm cells + cortico-therapy; G6 mares inseminated with 800 x 106 frozen-thawed sperm cells + 20 mL seminal plasma + cortico-therapy. The therapy with 0,1 mg/kg of prednizolone acetate (IM) (Predef ), was performed when the mares presented follicles with 35 mm and uterine edema. At this moment the ovulation was induced with human corionic gonadotrophin (hCG), and the corticoid administrations repeated every 12 hours until ovulation. In G5 and G6 when the animals were treated with corticoid, it was observed a significant decrease on the neutrophil function, analyzed by the tetrazolio nitroazul test (NBT). The infusion of seminal plasma did not modify the uterine inflammatory response in any of the performed tests. For the fertility test, two groups of inseminated mares were used. On the first one, mares with no history of intense uterine inflammatory response after insemination were used. No statistic differences were observed on pregnancy results. However, on the second group of fertility test when problem mares, with history of post-insemination endometritis, were used, the corticoid-treated group presented a significant high pregnancy rate when compared with the control group (67% and 0% respectively). The cortico-therapy showed to be a safe method with no side effects on treated animals, representing an adequate choice for animals... (Complete abstract, click electronic address below).
Descrição
Palavras-chave
Reprodução animal, Sêmen congelado, Plasma seminal, Corticóide
Como citar
DELL'AQUA JUNIOR, José Antonio. Efeito da córtico-terapia e plasma seminal na resposta inflamatória e fertilidade de éguas submetidas à inseminação arterial. 2004. 101 f. Tese(doutorado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, 2004.