Show simple item record

dc.contributor.authorGomes, Elisângela Soares [UNESP]
dc.contributor.authorSchuch, Viviane [UNESP]
dc.contributor.authorLemos, Eliana Gertrudes De Macedo [UNESP]
dc.date.accessioned2014-10-01T13:08:48Z
dc.date.available2014-10-01T13:08:48Z
dc.date.issued2013-12-01
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822013000400002
dc.identifier.citationBrazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 44, n. 4, p. 1007-1034, 2013.
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11449/110081
dc.description.abstractThe discovery of secondary metabolites produced by microorganisms (e.g., penicillin in 1928) and the beginning of their industrial application (1940) opened new doors to what has been the main medication source for the treatment of infectious diseases and tumors. In fact, approximately 80 years after the discovery of the first antibiotic compound, and despite all of the warnings about the failure of the goose that laid the golden egg, the potential of this wealth is still inexorable: simply adjust the focus from micro to nano, that means changing the look from microorganisms to nanograms of DNA. Then, the search for new drugs, driven by genetic engineering combined with metagenomic strategies, shows us a way to bypass the barriers imposed by methodologies limited to isolation and culturing. However, we are far from solving the problem of supplying new molecules that are effective against the plasticity of multi- or pan-drug-resistant pathogens. Although the first advances in genetic engineering date back to 1990, there is still a lack of high-throughput methods to speed up the screening of new genes and design new molecules by recombination of pathways. In addition, it is necessary an increase in the variety of heterologous hosts and improvements throughout the full drug discovery pipeline. Among numerous studies focused on this subject, those on polyketide antibiotics stand out for the large technical-scientific efforts that established novel solutions for the transfer/engineering of major metabolic pathways using transposons and other episomes, overcoming one of the main methodological constraints for the heterologous expression of major pathways. In silico prediction analysis of three-dimensional enzymatic structures and advances in sequencing technologies have expanded access to the metabolic potential of microorganisms.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipGraduate Program on Microbiologia Agropecuaria from UNESP-FCAV
dc.format.extent1007-1034
dc.language.isoeng
dc.publisherSociedade Brasileira de Microbiologia
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceSciELO
dc.subjectenvironmental samplesen
dc.subjectpharmacologyen
dc.subjectPKSsen
dc.subjectnew drugsen
dc.titleBiotechnology of polyketides: new breath of life for the novel antibiotic genetic pathways discovery through metagenomicsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias Departamento de Tecnologia
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias Departamento de Tecnologia
dc.identifier.doi10.1590/S1517-83822013000400002
dc.identifier.scieloS1517-83822013000400002
dc.identifier.wosWOS:000333959600002
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
dc.identifier.fileS1517-83822013000400002.pdf
dc.identifier.lattes3902020936480943
dc.identifier.orcid0000-0002-1119-7748[3]
unesp.author.lattes3902020936480943[3]
unesp.author.orcid0000-0003-4401-3869[1]
unesp.author.orcid0000-0002-1119-7748[3]
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
Localize o texto completo

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record