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dc.contributor.authorRibeiro-Peres, A. [UNESP]
dc.contributor.authorMunita-Barbosa, L.
dc.contributor.authorYumi-Kanazawa, M.
dc.contributor.authorMello-Martins, M. I.
dc.contributor.authorFerreira de Souza, F. [UNESP]
dc.date.accessioned2014-12-03T13:08:41Z
dc.date.available2014-12-03T13:08:41Z
dc.date.issued2014-01-01
dc.identifierhttp://dx.doi.org/10.4067/S0301-732X2014000100005
dc.identifier.citationArchivos de Medicina Veterinaria. Valdivia: Univ Austral Chile, Fac Ciencias Veterinarias, v. 46, n. 1, p. 31-38, 2014.
dc.identifier.issn0301-732X
dc.identifier.urihttp://hdl.handle.net/11449/111483
dc.description.abstractCryopreservation of sperm is important to preserve the gerrnplasm from animals of genetic value, which can die unexpectedly. This study compares conventional and automated methods of cryopreservation of spermatozoa obtained from the epididymis of bulls post-mortem. Twenty-two epididymides were obtained from a commercial slaughterhouse. Spermatozoa were collected from the tail of the epididymis using the retrograde flow technique. Thus, the samples, which were diluted in 10 ml of extender without glycerol (Botubov (R) I, Botupharma, Botucatu, SP, Brazil), were evaluated on motility, sperm vigor, structural and functional (swelling hypoosmotic test) membrane integrity, mitochondrial activity, sperm viability and ADN fragmentation. The samples were divided into two aliquots and diluted in extender with glycerol (Botubov (R) II, Botupharma, Botucatu, SP, Brazil) at a concentration of 50x10(6) motile sperm/0.5 French straws. One sample was frozen by the conventional method (4 hours at 5 degrees C, in a refrigerator and 20 min in nitrogen vapor) and the other by the automated method (Cryogen (R) Dualflex, Neovet, Uberaba, MG, Brazil). The parameters were higher in all the tests of fresh sperm samples, with the exception of the swelling hypoosmotic test, which showed no significant difference when the results were compared with sperm frozen by the conventional method. The average motility of fresh spermatozoa was 74%, and conventional and automated averages were 29 and 25%, respectively. Therefore, although cryopreservation techniques reduce sperm quality parameters, the viability of the sperm is maintained, and these methods can be used to preserve sperm.en
dc.format.extent31-38
dc.language.isospa
dc.publisherUniv Austral Chile, Fac Ciencias Veterinarias
dc.relation.ispartofArchivos de Medicina Veterinária
dc.sourceWeb of Science
dc.subjectspermen
dc.subjectfrozen-spermen
dc.subjectbullen
dc.subjectepididymisen
dc.titleCryopreservation of bovine spermatozoa from the epididymal tail using conventional and automated methodsen
dc.typeArtigo
dcterms.rightsHolderUniv Austral Chile, Fac Ciencias Veterinarias
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniv Franca
dc.contributor.institutionUniv Londrina
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.affiliationUniv Franca, Franca, Brazil
dc.description.affiliationUniv Londrina, Dept Ciencias Clin, Londrina, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.identifier.wosWOS:000337121100005
dc.rights.accessRightsAcesso aberto
dc.identifier.fileS0301-732X2014000100005.pdf
unesp.author.orcid0000-0003-4721-1801[5]
dc.relation.ispartofjcr0.236
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