Brief Report: The lincRNA Hotair Is Required for Epithelial-to-Mesenchymal Transition and Stemness Maintenance of Cancer Cell Lines
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Data
2013-12-01
Autores
Alves, Cleidson Padua
Fonseca, Aline Simoneti
Muys, Bruna Rodrigues
Bueno, Rafaela de Barros e Lima
Buerger, Matheus Carvalho
Souza, Jorge E. S. de
Valente, Valeria [UNESP]
Zago, Marco Antonio
Silva, Wilson Araujo
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Editor
Wiley-Blackwell
Resumo
Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133(+)/CD44(+)) presents much higher levels of Hotair when compared with the non-stem cell subpopulation. These results indicate that Hotair acts as a key regulator that controls the multiple signaling mechanisms involved in EMT. Altogether, our data suggest that the role of Hotair in tumorigenesis occurs through EMT triggering and stemness acquisition.
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Palavras-chave
Cancer stem cell, Epithelial-to-mesenchymal transition, LincRNA, Hotair
Como citar
Stem Cells. Hoboken: Wiley-blackwell, v. 31, n. 12, p. 2827-2832, 2013.