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dc.contributor.authorCosta-Orlandi, C. B. [UNESP]
dc.contributor.authorSardi, J. C. O. [UNESP]
dc.contributor.authorSantos, C. T. [UNESP]
dc.contributor.authorFusco-Almeida, A. M. [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.date.accessioned2014-12-03T13:11:42Z
dc.date.available2014-12-03T13:11:42Z
dc.date.issued2014-01-01
dc.identifierhttp://dx.doi.org/10.1080/08927014.2014.919282
dc.identifier.citationBiofouling. Abingdon: Taylor & Francis Ltd, v. 30, n. 6, p. 719-727, 2014.
dc.identifier.issn0892-7014
dc.identifier.urihttp://hdl.handle.net/11449/113434
dc.description.abstractDermatophytes are fungi responsible for a disease known as dermatophytosis. Biofilms are sessile microbial communities surrounded by extracellular polymeric substances (EPS) with increased resistance to antimicrobial agents and host defenses. This paper describes, for the first time, the characteristics of Trichophyton rubrum and T. mentagrophytes biofilms. Biofilm formation was analyzed by light microscopy, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) as well as by staining with crystal violet and safranin. Metabolic activity was determined using the XTT reduction assay. Both species were able to form mature biofilms in 72 h. T. rubrum biofilm produced more biomass and EPS and was denser than T. mentagrophytes biofilm. The SEM results demonstrated a coordinated network of hyphae in all directions, embedded within EPS in some areas. Research and characterization of biofilms formed by dermatophytes may contribute to the search of new drugs for the treatment of these mycoses and might inform future revisions with respect to the dose and duration of treatment of currently available antifungals.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipPADC FCFAR UNESP (Programa de Desenvolvimento Cientifico - School of Pharmaceutical Sciences-UNESP
dc.format.extent719-727
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiofouling
dc.sourceWeb of Science
dc.subjectTrichophyton rubrumen
dc.subjectT. mentagrophytesen
dc.subjectbiofilmen
dc.subjectdermatophytesen
dc.subjectSEMen
dc.subjectCLSMen
dc.titleIn vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilmsen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Clin Mycol Lab, Dept Clin Anal, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Clin Mycol Lab, Dept Clin Anal, BR-14801902 Araraquara, SP, Brazil
dc.identifier.doi10.1080/08927014.2014.919282
dc.identifier.wosWOS:000337965600007
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdCNPq: 141073/2012-8
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.orcid0000-0002-8059-0826
unesp.author.lattes3716273524139678[4]
unesp.author.orcid0000-0002-5752-6367[1]
unesp.author.orcid0000-0002-8059-0826[5]
unesp.author.orcid0000-0002-2115-8988[4]
dc.relation.ispartofjcr2.786
dc.relation.ispartofsjr0,835
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