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dc.contributor.authorCalixto, Giovana [UNESP]
dc.contributor.authorDomingos, Luciana Elisete
dc.contributor.authorFabri, Nathalie Thomazini
dc.contributor.authorCarmo, Thais Adriana do
dc.contributor.authorMoreno, Andreia de Haro [UNESP]
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.identifier.citationCurrent Analytical Chemistry. Sharjah: Bentham Science Publ Ltd, v. 10, n. 3, p. 443-448, 2014.
dc.description.abstractAn innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear ( r(2) = 0.9985), precise ( CV = 1.32%) and accurate ( 100.9 +/- 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCurrent Analytical Chemistry
dc.sourceWeb of Science
dc.subjectAgar diffusion methoden
dc.subjectBordetella bronchisepticaen
dc.subjectcolistin sulphateen
dc.subjectmethod validationen
dc.subjectmicrobiological assayen
dc.subjectin vitro quantificationen
dc.titleMicrobiological Assay for the Determination of Colistin Sulphateen
dcterms.rightsHolderBentham Science Publ Ltd
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniv Metodista Piracicaba
dc.description.affiliationSao Paulo State Univ, Dept Drugs & Pharmaceut, Sch Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Metodista Piracicaba, Sch Hlth Sci, BR-13400911 Piracicaba, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Drugs & Pharmaceut, Sch Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
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