Common Descent of B Chromosomes in Two Species of the Fish Genus Prochilodus (Characiformes, Prochilodontidae)
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Data
2013-01-01
Autores
Voltolin, T. A. [UNESP]
Alves, J. C. Pansonato [UNESP]
Senhorini, J. A.
Foresti, F. [UNESP]
Camacho, J. P. M.
Porto-Foresti, F. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Karger
Resumo
To ascertain the origin of B chromosomes in 2 fish species of the genus Prochilodus, i.e. P. lineatus and P. nigricans, we microdissected them and generated B-specific DNA probes. These probes were used to perform chromosome painting in both species and in 3 further ones belonging to the same genus (P. argenteus, P. brevis and P. costatus). Both probes hybridized with the B chromosomes in P. lineatus and P. nigricans, but with none of the chromosomes in the 5 species. This indicates that the B chromosomes have low similarity with DNAs located in the A chromosomes and suggests the possibility that the B chromosomes in the 2 species have a common origin. The most parsimonious explanation would imply intergeneric hybridization in an ancestor of P. lineatus and P. nigricans yielding the B chromosome as a byproduct, which remained in these 2 species after their phylogenetic origin, but was perhaps lost in other Prochilodus species. This hypothesis predicts that B chromosomes are old genomic elements in this genus, and this could be tested once a species from a relative genus would be found showing homology of its A chromosomes with the B-probes employed here, through a comparison of B chromosome DNA sequences with those in the A chromosomes of this other species. (C) 2013 S. Karger AG, Basel
Descrição
Palavras-chave
B chromosomes, Chromosome painting, Interspecific origin, Intraspecific origin, Microdissection, Prochilodus, Supernumerary chromosomes
Como citar
Cytogenetic And Genome Research. Basel: Karger, v. 141, n. 2-3, p. 206-211, 2013.