Show simple item record

dc.contributor.authorLima-Leopoldo, A. P. [UNESP]
dc.contributor.authorSugizaki, M. M. [UNESP]
dc.contributor.authorLeopoldo, A. S. [UNESP]
dc.contributor.authorCarvalho, R. F. [UNESP]
dc.contributor.authorNogueira, Célia Regina [UNESP]
dc.contributor.authorNascimento, A. F. [UNESP]
dc.contributor.authorMartinez, P. F. [UNESP]
dc.contributor.authorLuvizotto, R. A. M. [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.date.accessioned2014-05-20T13:33:37Z
dc.date.available2014-05-20T13:33:37Z
dc.date.issued2008-07-01
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2008000700011
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 7, p. 615-620, 2008.
dc.identifier.issn0100-879X
dc.identifier.urihttp://hdl.handle.net/11449/11512
dc.description.abstractObesity is a complex multifactorial disorder that is often associated with cardiovascular diseases. Research on experimental models has suggested that cardiac dysfunction in obesity might be related to alterations in myocardial intracellular calcium (Ca2+) handling. However, information about the expression of Ca2+-related genes that lead to this abnormality is scarce. We evaluated the effects of obesity induced by a high-fat diet in the expression of Ca2+-related genes, focusing the L-type Ca2+ channel (Cacna1c), sarcolemmal Na+/Ca2+ exchanger (NCX), sarcoplasmic reticulum Ca2+ ATPase (SERCA2a), ryanodine receptor (RyR2), and phospholamban (PLB) mRNA in rat myocardium. Male 30-day-old Wistar rats were fed a standard (control) or high-fat diet (obese) for 15 weeks. Obesity was defined as increased percent of body fat in carcass. The mRNA expression of Ca2+-related genes in the left ventricle was measured by RT-PCR. Compared with control rats, the obese rats had increased percent of body fat, area under the curve for glucose, and leptin and insulin plasma concentrations. Obesity also caused an increase in the levels of SERCA2a, RyR2 and PLB mRNA (P < 0.05) but did not modify the mRNA levels of Cacna1c and NCX. These findings show that obesity induced by high-fat diet causes cardiac upregulation of Ca2+ transport_related genes in the sarcoplasmic reticulum.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent615-620
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.sourceSciELO
dc.subjectObesityen
dc.subjectHigh-fat dieten
dc.subjectRat hearten
dc.subjectCa2+ cyclingen
dc.subjectmRNAen
dc.subjectCa2+-related genesen
dc.titleObesity induces upregulation of genes involved in myocardial Ca2+ handlingen
dc.typeArtigo
dcterms.rightsHolderAssoc Bras Divulg Cientifica
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Clínica Médica
dc.description.affiliationUniversidade Estadual Paulista Departamento de Morfologia
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Clínica Médica
dc.description.affiliationUnespUniversidade Estadual Paulista Departamento de Morfologia
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística
dc.identifier.doi10.1590/S0100-879X2008000700011
dc.identifier.scieloS0100-879X2008000700011
dc.identifier.wosWOS:000259326100011
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 07/53267-3
dc.description.sponsorshipIdCNPq: 130572/2005-5
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
dc.identifier.fileS0100-879X2008000700011.pdf
dc.identifier.lattes5406518799128485
dc.identifier.lattes9418970103564137
dc.identifier.lattes8727897080522289
dc.identifier.orcid0000-0002-4901-7714
unesp.author.lattes5406518799128485
unesp.author.lattes9418970103564137
unesp.author.lattes8727897080522289
unesp.author.orcid0000-0002-4901-7714[4]
dc.relation.ispartofjcr1.492
Localize o texto completo

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record