Nicho espermatogonial em Cyprinus carpio e papel do Amh na espermaogênese
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Data
2014-08-01
Autores
Oliveira, Marcos Antonio de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Neste estudo, caracterizamos o nicho espermatogonial em carpa avaliando a expressão do amh em testículos com alta ou baixa expressão. Para tanto, vinte carpas comum adultas receberam pulsos de BrdU (5-bromo-2'-deoxiuridina), durante 3 dias consecutivos (2 injeções intraperitoneal/dia), e os testículo foram amostrados 4 horas, 1, 2 e 3 semanas após o último pulso. As espermatogônias do tipo A indiferenciada foram as únicas células germinativas capazes de reter BrdU após 3 semanas. Curiosamente, o BrdU diluiu mais rapidamente entre as espermatogônias troncoativa enquanto que nas espermatogônias troncoreserva, manteve-se estável. As espermatogônias tronco -reserva? foram localizados próximas do interstício, enquanto as espermatogônias tronco -ativa? foram encontrados na região intertubular. Algumas células de Sertoli foram BrdU-positivas depois de 3 semanas de pulso. Entre as células de Sertoli BrdU-positivas, ~ 30-40% estão livres, enquanto que ~ 60-70% estão associadas com células germinativas. Isto sugere a existência de células de Sertoli tronco, que estão localizados no nicho espermatogonial. O gene amh foi clonado e caracterizado em carpa comum e sua expressão ocorre nas células de Sertoli que envolvem espermatogônias indiferenciadas. Os hormônios folículo-estimulante e estrógeno diminuem a expressão do amh. Em animais que possuem alta expressão de amh, possuem pouca atividade proliferativa, já com elevada proliferação, os níveis de amh diminuem significativamente. Desta forma, sugere-se que a dimunuição do amh, cria uma condição permissiva para proliferação e diferenciação das espermatogônias em carpa. Tais resultados servirão de base para aplicar este conhecimento para mediar a puberdade precoce em espécies nativas/exóticas de importância econômica na aquicultura
In this study, we start characterizing the spermatogonial niche in carp by evaluating amh expression in testes with high or low proliferative activity. 20 adult carp received pulses of BrdU (5-Bromo-2’-deoxyuridine), during 3 consecutive days (2 intraperitoneal injections/day), and testis was sample dafter 4 hours, and 1, 2 and 3 weeks after the last pulse. Type Aund were the only germ cells able to retain BrdU after a long period of chase (3 weeks). Interestingly, BrdU disappeared more quickly from -active? stem cell while in -reserve? stem cell, it remained stable. The -reserve? stem cell label-retaining cells were located near the interstitium, while -active? stem cell were found in the intertubular area. Some Sertoli cells were BrdU-positive after 3 weeks of pulse. Among the slow-dividing, BrdU-positive Sertoli cells, ~30-40% are -free?, and ~60-70% associated with germ cells. This suggests the existence of Sertoli stem cells which are located in the spermatogonial niche. The amh gene was cloned and characterized in common carp, and the amh is expressed in Sertoli cells involving undifferentiated spermatogonia. The follicle stimulating hormone and estrogen decrease amh expression. It was also shown that animals with high expression of amh, have low proliferative activity. When the testes with high proliferation, amh levels decrease significantly. Thus, it is suggested that counts down the amh creates a permissive condition for proliferation and differentiation of spermatogonial in carp. These results provide a basis for applying this knowledge to mediate early puberty in exotic/native species economically important in aquaculture
In this study, we start characterizing the spermatogonial niche in carp by evaluating amh expression in testes with high or low proliferative activity. 20 adult carp received pulses of BrdU (5-Bromo-2’-deoxyuridine), during 3 consecutive days (2 intraperitoneal injections/day), and testis was sample dafter 4 hours, and 1, 2 and 3 weeks after the last pulse. Type Aund were the only germ cells able to retain BrdU after a long period of chase (3 weeks). Interestingly, BrdU disappeared more quickly from -active? stem cell while in -reserve? stem cell, it remained stable. The -reserve? stem cell label-retaining cells were located near the interstitium, while -active? stem cell were found in the intertubular area. Some Sertoli cells were BrdU-positive after 3 weeks of pulse. Among the slow-dividing, BrdU-positive Sertoli cells, ~30-40% are -free?, and ~60-70% associated with germ cells. This suggests the existence of Sertoli stem cells which are located in the spermatogonial niche. The amh gene was cloned and characterized in common carp, and the amh is expressed in Sertoli cells involving undifferentiated spermatogonia. The follicle stimulating hormone and estrogen decrease amh expression. It was also shown that animals with high expression of amh, have low proliferative activity. When the testes with high proliferation, amh levels decrease significantly. Thus, it is suggested that counts down the amh creates a permissive condition for proliferation and differentiation of spermatogonial in carp. These results provide a basis for applying this knowledge to mediate early puberty in exotic/native species economically important in aquaculture
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Palavras-chave
Peixe, Carpa (Peixe), Peixe - Reprodução, Sertoli, Celulas de, Hormonios, Fishes
Como citar
OLIVEIRA, Marcos Antonio de. Nicho espermatogonial em Cyprinus carpio e papel do Amh na espermaogênese. 2014. i, 84 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Centro de Aquicultura de Jaboticabal, 2014.