Optimisation of a sample preparation method for the determination of fumonisin B-1 in rice
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Data
2014-09-01
Autores
Petrarca, Mateus Henrique [UNESP]
Rodrigues, Maria Isabel
Rossi, Elizeu Antonio [UNESP]
Sylos, Célia Maria de [UNESP]
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Editor
Elsevier B.V.
Resumo
A simple, rapid and cost-effective sample preparation method for the determination of fumonisin B-1 in rice was optimised using a strategy of sequential experimental designs. Initially, a Plackett-Burman design was applied to select the statistically significant variables for the determination of fumonisin B-1, and then, a central composite rotatable design was used to define the optimal conditions of these variables. The method involves extraction with a 50% acetonitrile aqueous solution and glacial acetic acid, liquid-liquid partitioning with addition of anhydrous sodium sulphate and sodium chloride, followed by dispersive SPE clean-up with diatomaceous earth. The final extract was analysed by HPLC-FLD after precolumn derivatisation with ortho-phthaldialdehyde. The optimised method was validated for selectivity, linearity, matrix effect, limits of detection and quantification, trueness, and precision, and then applied to commercial samples of polished rice. This is the first report of the occurrence of fumonisin B-1 in commercial samples of polished rice from the Southeast region of Brazil. (C) 2014 Elsevier Ltd. All rights reserved.
Descrição
Palavras-chave
Fumonisin B-1, Rice, Sample preparation, Plackett-Burman design, Response surface methodology, In-house validation
Como citar
Food Chemistry. Oxford: Elsevier Sci Ltd, v. 158, p. 270-277, 2014.