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dc.contributor.authorValsechi, Marina Curado [UNESP]
dc.contributor.authorBortolozo Oliveira, Ana Beatriz [UNESP]
dc.contributor.authorGiacometti Conceicao, Andre Luis [UNESP]
dc.contributor.authorStuqui, Bruna [UNESP]
dc.contributor.authorCandido, Natalia Maria [UNESP]
dc.contributor.authorScarin Provazzi, Paola Jocelan [UNESP]
dc.contributor.authorAraujo, Luiza Ferreira de
dc.contributor.authorSilva, Wilson Araujo
dc.contributor.authorCalmon, Marilia de Freitas [UNESP]
dc.contributor.authorRahal, Paula [UNESP]
dc.date.accessioned2015-03-18T15:55:48Z
dc.date.available2015-03-18T15:55:48Z
dc.date.issued2014-08-29
dc.identifierhttp://dx.doi.org/10.1186/1471-2407-14-631
dc.identifier.citationBmc Cancer. London: Biomed Central Ltd, v. 14, 11 p., 2014.
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11449/117313
dc.description.abstractBackground: Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma.Methods: Five clear cell renal cell carcinoma cell lines and carcinoma samples were used to analyze GPC3 mRNA expression (qRT-PCR). Then, representative cell lines, one primary renal carcinoma (786-O) and one metastatic renal carcinoma (ACHN), were chosen to carry out functional studies. We constructed a GPC3 expression vector and transfected the renal carcinoma cell lines, 786-O and ACHN. GPC3 overexpression was analyzed using qRT-PCR and immunocytochemistry. We evaluated cell proliferation using MTT and colony formation assays. Flow cytometry was used to evaluate apoptosis and perform cell cycle analyses.Results: We observed that GPC3 is downregulated in clear cell renal cell carcinoma samples and cell lines compared with normal renal samples. GPC3 mRNA expression and protein levels in 786-O and ACHN cell lines increased after transfection with the GPC3 expression construct, and the cell proliferation rate decreased in both cell lines following overexpression of GPC3. Further, apoptosis was not induced in the renal cell carcinoma cell lines overexpressing GPC3, and there was an increase in the cell population during the G1 phase in the cell cycle.Conclusion: We suggest that the GPC3 gene reduces the rate of cell proliferation through cell cycle arrest during the G1 phase in renal cell carcinoma.en
dc.format.extent11
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Cancer
dc.sourceWeb of Science
dc.subjectGPC3en
dc.subjectCell linesen
dc.subjectCell proliferationen
dc.subjectRenal carcinomaen
dc.subjectTransfectionen
dc.titleGPC3 reduces cell proliferation in renal carcinoma cell linesen
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciencias Exatas IBILCE, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, CISBi NAP, Ctr Integrat Syst Biol, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciencias Exatas IBILCE, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.identifier.doi10.1186/1471-2407-14-631
dc.identifier.wosWOS:000341655800001
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileWOS000341655800001.pdf
dc.identifier.lattes7991082362671212
dc.identifier.lattes9165601469436240
dc.identifier.orcid0000-0001-5693-6148
unesp.author.lattes7991082362671212[10]
unesp.author.lattes9165601469436240
unesp.author.orcid0000-0001-5693-6148[10]
dc.relation.ispartofjcr3.288
dc.relation.ispartofsjr1,464
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