Show simple item record

dc.contributor.authorAkagi, Erica M.
dc.contributor.authorLavorato-Rocha, Andre M.
dc.contributor.authorMaia, Beatriz de Melo
dc.contributor.authorRodrigues, Iara S.
dc.contributor.authorCarvalho, Katia C.
dc.contributor.authorStiepcich, Monica M.
dc.contributor.authorBaiocchi, Glauco
dc.contributor.authorSato-Kuwabara, Yukie
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.authorSoares, Fernando A.
dc.contributor.authorRocha, Rafael M.
dc.date.accessioned2015-03-18T15:55:48Z
dc.date.available2015-03-18T15:55:48Z
dc.date.issued2014-11-07
dc.identifierhttp://dx.doi.org/10.1186/1471-2407-14-822
dc.identifier.citationBmc Cancer. London: Biomed Central Ltd, v. 14, 10 p., 2014.
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11449/117314
dc.description.abstractBackground: Vulvar carcinoma is an infrequent tumour, accounting for fewer than 3% of all malignant tumours that affect women, but its incidence is rising in the past few decades. In young women, the manifestation of the vulvar carcinoma is often linked to risk factors such as smoking and HPV infection, but most cases develop in women aged over 50 years through poorly understood genetic mechanisms. Rho-associated coiled-coil-containing protein kinase 1 (ROCK1) has been implicated in many cellular processes, but its function in vulvar cancer has never been examined. In this study, we aimed to determine the prognostic value of ROCK1 gene and protein analysis in vulvar squamous cell carcinoma (VSCC).Methods: ROCK1 expression levels were measured in 16 vulvar tumour samples and adjacent normal tissue by qRT-PCR. Further, 96 VSCC samples were examined by immunohistochemistry (IHC) to confirm the involvement of ROCK1 in the disease. The molecular and pathological results were correlated with the clinical data of the patients. Sixteen fresh VSCC samples were analyzed by array-based comparative genomic hybridization (aCGH).Results: In each pair of samples, ROCK1 levels were higher by qRT-PCR in normal tissue compared with the tumour samples (p = 0.016). By IHC, 100% of invasive front areas of the tumour and 95.8% of central tumour areas were positive for ROCK1. Greater expression of ROCK1 was associated with the absence of lymph node metastasis (p = 0.022) and a lower depth of invasion (p = 0.002). In addition, higher ROCK1 levels correlated with greater recurrence-free survival (p = 0.001). Loss of ROCK1 was independently linked to worse cancer-specific survival (p = 0.0054) by multivariate analysis. This finding was validated by IHC, which demonstrated enhanced protein expression in normal versus tumour tissue (p < 0.001). By aCGH, 42.9% of samples showed a gain in copy number of the ROCK1 gene.Conclusions: ROCK1 is lower expressed in tumour tissue when compared with adjacent normal vulvar epithelia. In an independent sample set of VSCCs, lower expression levels of ROCK1 correlated with worse survival rates and a poor prognosis. These findings provide important information for the clinical management of vulvar cancer.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent10
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Cancer
dc.sourceWeb of Science
dc.subjectVulvar carcinomaen
dc.subjectROCK1en
dc.subjectqRT-PCRen
dc.subjectImmunohistochemistryen
dc.subjectaCGHen
dc.subjectPrognosisen
dc.titleROCK1 as a novel prognostic marker in vulvar canceren
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
dc.contributor.institutionAC Camargo Canc Ctr
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFleury Inst
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationAC Camargo Canc Ctr, Mol Morphol Lab, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Obstet & Gynecol, Sao Paulo, Brazil
dc.description.affiliationFleury Inst, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Dept Gynecol Oncol, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Dept Anat Pathol, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, NeoGene Lab, Sao Paulo, Brazil
dc.description.affiliationUNESP, Fac Med, Dept Urol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med, Dept Urol, Botucatu, SP, Brazil
dc.identifier.doi10.1186/1471-2407-14-822
dc.identifier.wosWOS:000345161300001
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
dc.identifier.fileWOS000345161300001.pdf
dc.identifier.lattes2259986546265579
unesp.author.lattes2259986546265579
dc.relation.ispartofjcr3.288
dc.relation.ispartofsjr1,464
Localize o texto completo

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record