Fabrication of microenvironments with different geometrical features for cell growth studies
Nenhuma Miniatura disponível
Data
2014-11-01
Autores
Avila, O. I.
Otuka, A. J. G.
Tribuzi, V.
Freitas, Laura Marise de [UNESP]
Serafim, Rodolfo B. [UNESP]
Moraes, M. H.
Espreafico, E. M.
Valente, Valeria [UNESP]
Fontana, Carla Raquel [UNESP]
Mendonca, C. R.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Japan Laser Processing Soc
Resumo
Studies of cell development in artificial microenvironments can contribute to understanding a series of physiological mechanisms that might be influenced by geometrical features of the microenvironment itself. In this work we applied two-photon polymerization to fabricate three-dimensional microenvironments composed of a matrix arrangement of microstructures (circular and square cross-sections pillars) with distinct spacing. Such microenvironments were used to evaluate the growth of Michigan Cancer Foundation-7 (MCF-7) cells that are commonly used as a model system to investigate fundamental aspects of the tumor biology. Our results reveal that the cell density decreases as the distance between structures in the environment is increased. Additionally, cell growth shows slightly better results for the microenvironments composed of circular cross-section structures.
Descrição
Palavras-chave
Two-photon absortion, microfabrication, photopolimerization, Michigan Cancer Foundation-7, Green Fluorescent Protein
Como citar
Journal Of Laser Micro Nanoengineering. Osaka: Japan Laser Processing Soc, v. 9, n. 3, p. 248-251, 2014.