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dc.contributor.authorBarbizan, Roberta
dc.contributor.authorCastro, Mateus V.
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorOliveira, Alexandre L. R.
dc.date.accessioned2015-03-18T15:56:21Z
dc.date.available2015-03-18T15:56:21Z
dc.date.issued2014-11-01
dc.identifierhttp://dx.doi.org/10.3390/ijms151119535
dc.identifier.citationInternational Journal Of Molecular Sciences. Basel: Mdpi Ag, v. 15, n. 11, p. 19535-19551, 2014.
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11449/117515
dc.description.abstractWe recently proposed a new surgical approach to treat ventral root avulsion, resulting in motoneuron protection. The present work combined such a surgical approach with bone marrow mononuclear cells (MC) therapy. Therefore, MC were added to the site of reimplantation. Female Lewis rats (seven weeks old) were subjected to unilateral ventral root avulsion (VRA) at L4, L5 and L6 levels and divided into the following groups (n = 5 for each group): Avulsion, sealant reimplanted roots and sealant reimplanted roots plus MC. After four weeks and 12 weeks post-surgery, the lumbar intumescences were processed by transmission electron microscopy, to analyze synaptic inputs to the repaired a motoneurons. Also, the ipsi and contralateral sciatic nerves were processed for axon counting and morphometry. The ultrastructural results indicated a significant preservation of inhibitory pre-synaptic boutons in the groups repaired with sealant alone and associated with MC therapy. Moreover, the average number of axons was higher in treated groups when compared to avulsion only. Complementary to the fiber counting, the morphometric analysis of axonal diameter and "g" ratio demonstrated that root reimplantation improved the motor component recovery. In conclusion, the data herein demonstrate that root reimplantation at the lesion site may be considered a therapeutic approach, following proximal lesions in the interface of central nervous system (CNS) and peripheral nervous system (PNS), and that MC therapy does not further improve the regenerative recovery, up to 12 weeks post lesion.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent19535-19551
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofInternational Journal Of Molecular Sciences
dc.sourceWeb of Science
dc.subjectventral root avulsionen
dc.subjectfibrin sealanten
dc.subjectroot reimplantationen
dc.subjectmononuclear cellsen
dc.subjectultrastructureen
dc.titleLong-Term Spinal Ventral Root Reimplantation, but not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowthen
dc.typeArtigo
dcterms.rightsHolderMdpi Ag
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Campinas UNICAMP, Dept Struct & Funct Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, Brazil
dc.identifier.doi10.3390/ijms151119535
dc.identifier.wosWOS:000345529200015
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 14/06892-3
dc.description.sponsorshipIdFAPESP: 12/19646-6
dc.description.sponsorshipIdFAPESP: 12/08101-8
dc.description.sponsorshipIdFAPESP: 11/23236-4
dc.description.sponsorshipIdFAPESP: 09/53846-9
dc.description.sponsorshipIdCNPq: 563582/2010-3
dc.description.sponsorshipIdCAPES: AUXPE Toxinologia 1219/2011
dc.description.sponsorshipIdCAPES: 23038.000823/2011-21
dc.description.sponsorshipIdCAPES: 23038.005536/2012-31
dc.description.sponsorshipIdCNPq: 300552/2013-9
dc.description.sponsorshipIdCNPq: 310207/2011-8
dc.identifier.fileWOS000345529200015.pdf
unesp.author.orcid0000-0002-9855-5594[4]
dc.relation.ispartofjcr3.687
dc.relation.ispartofsjr1,260
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