Inhibitory effect of silibinin on tumour necrosis factor-alpha and hydrogen peroxide production by human monocytes
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Data
2010-01-01
Autores
Bannwart-Castro, Camila F. [UNESP]
Peraçoli, José Carlos [UNESP]
Nakaira-Takahagi, E. [UNESP]
Peraçoli, Maria Terezinha Serrão [UNESP]
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Editor
Taylor & Francis Ltd
Resumo
Silibinin is a chemically defined flavonoid and the main active component of silymarin, a polyphenolic complex from Silybum marianum, which has anti-inflammatory, hepatoprotective and anticarcinogenic properties. Monocytes obtained from healthy individuals were incubated with silibinin to evaluate cell viability, hydrogen peroxide (H2O2) release and tumour necrosis factor-alpha (TNF-) production by these cells. The duration of treatment and different silibinin concentrations had no significant effect on cell viability. Monocytes showed a dose-dependent inhibitory effect on H2O2 release by phorbol myristate acetate-stimulated monocytes in silibinin concentrations ranging from 6.25 to 50 mu g mL-1. Significant inhibition of TNF- production by lipopolysaccharide-stimulated monocytes was observed at concentrations of 12.5, 50 and 100 mu g mL-1 of silibinin. These results suggest that silibinin exerts antioxidant and anti-inflammatory properties on human monocytes through an inhibitory effect on H2O2 release and on TNF- production, respectively.
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Palavras-chave
hydrogen peroxide, TNF-, flavonoid, silibinin, monocytes
Como citar
Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 24, n. 18, p. 1747-1757, 2010.