Low toxic effects of a whitening strip to cultured pup cells
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Data
2013
Autores
Soares, Diana Gabriela de Souza [UNESP]
Pontes, Elaine Cristina Voltolini [UNESP]
Ribeiro, Ana Paula Dias
Basso, Fernanda Gonçalves [UNESP]
Hebling, Josimeri [UNESP]
Costa, Carlos Alberto de Souza [UNESP]
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Resumo
To assess the trans-enamel and trans-dentin toxicity of a 10% hydrogen peroxide (HP) whitening strip to odontoblast-like cells (MDPC-23). Methods: Enamel surfaces of enamel/dentin discs adapted to artificial pulp chambers were subjected to two 30-minute whitening strip applications to obtain indirect extracts (DMEM + bleaching components that diffused across enamel and dentin). The extracts were applied for 1 hour to the cells for 1 or 5 days. A bleaching gel with 35% HP was used as the positive control. Cell viability (MTT assay) and morphology (SEM) as well as the quantity of HP in the extracts were assessed. Results: Discrete cell viability reduction (21.9%) associated with slight alterations in cell morphology occurred after application of the extracts for 5 days to the MDPC-23 cells (Tukey's test; P< 0.05). Lower enamel/dentin diffusion of HP was observed after the use of the whitening strip compared with the bleaching gel (Mann-Whitney; P< 0.05).
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Palavras-chave
Randomized clinical-trial, Bleaching gel, In-vitro, Peroxide penetration, Carbamide peroxide, Hydrogen-peroxide, Mdpc-23 cells, Efficacy, Office, Products
Como citar
American Journal of Dentistry, v. 26, p. 283-285, 2013.