Responses of dental pulp cells to a less invasive bleaching technique applied to adhesive restored teeth
Nenhuma Miniatura disponível
Data
2015
Autores
Soares, Diana Gabriela de Souza
Sacono, Nancy Tomoko
Ribeiro, Ana Paula Dias
Basso, Fernanda Gonçalves
Scheffel, Débora Lopes Salles
Hebling, Josimeri [UNESP]
Costa, Carlos Alberto de Souza
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.
Descrição
Palavras-chave
Laboratory research, Toxicity, Odontoblasts, Tooth bleaching, Adhesives
Como citar
Journal of Adhesive Dentistry, v. 17, p. 155-161, 2015.