An investigation of Leishmania spp. in Didelphis spp. from urban and peri-urban areas in Bauru (São Paulo, Brazil)
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Data
2007-12-25
Autores
Bodini Santiago, Maria Emilia [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Fattori, Karina Reinaldo [UNESP]
Munari, Danisio Prado [UNESP]
de Fatima Michelin, Aparecida [UNESP]
Felix Lima, Valeria Marcal [UNESP]
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Editor
Elsevier B.V.
Resumo
Blood and bone marrow samples were taken from 112 Didelphis spp., collected between March 2005 and February 2006, from urban and peri-urban areas of Bauru, São Paulo State, Brazil, to evaluate the hypothesis that these animals might constitute a reservoir of Leishmania spp. Anti-Leishman ia ssp. antibodies were screened in the serum samples using an enzyme-linked immuno-sorbent assay (ELISA) and the polymerase chain reaction (PCR). PCR was performed on fragments of DNA samples from Leishmania spp. using primers 13A and 13B, and showed a positive outcome in 91.6% of the 112 samples tested. of the 107 samples analyzed by ELISA, 71 % were positive. Evidence of epidemiological risk factors such as a circulating parasite and freely moving vectors suggests that Didelphis spp. may participate in the transmission cycle of Leishmania spp. in Bauru. (c) 2007 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Leishmania spp., Didelphis spp., ELISA, PCR
Como citar
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 150, n. 4, p. 283-290, 2007.