Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil
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Data
2015-06-01
Autores
Candido, Marcelo
Alencar, Anna Luiza Farias
Queiroz, Sabrina Ribeiro de Almeida
Buzinaro, Maria da Glória [UNESP]
Munin, Flavia Simone
Godoy, Silvia Helena Seraphin de
Livonesi, Marcia Cristina
Fernandes, Andrezza Maria
Sousa, Ricardo Luiz Moro de
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Springer
Resumo
Bovine astrovirus (BoAstV) is associated with gastroenterical disorders such as diarrhea, particularly in neonates and immunocompromised animals. Its prevalence is > 60 % in the first five weeks of the animal's life. The aim of this study was to detect and perform a phylogenetic analysis of BoAstV in Brazilian cattle. A prevalence of 14.3 % of BoAstV in fecal samples from 272 head of cattle from different Brazilian states was detected, and 11 samples were analyzed by nucleotide sequencing. The majority of positive samples were obtained from diarrheic animals (p < 0.01). Phylogenetic analysis revealed that Brazilian samples were grouped in clades along with other BoAstV isolates. There was 74.3 %-96.5 % amino acid sequence similarity between the samples in this study and > 74.8 % when compared with reference samples for enteric BoAstV. Our results indicate, for the first time, the occurrence of BoAstV circulation in cattle from different regions of Brazil, prevalently in diarrheic calves.
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Palavras-chave
Mamastrovirus, Diarrhea, Cattle diseases, Molecular epidemiology
Como citar
Archives Of Virology. Wien: Springer Wien, v. 160, n. 6, p. 1519-1525, 2015.