Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum
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Data
2014-04-01
Autores
Santos-Ebinuma, Valeria Carvalho [UNESP]
Roberto, Ines Conceição
Simas Teixeira, Maria Francisca
Pessoa, Adalberto
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Microbiologia
Resumo
Safety issues related to the employment of synthetic colorants in different industrial segments have increased the interest in the production of colorants from natural sources, such as microorganisms. Improved cultivation technologies have allowed the use of microorganisms as an alternative source of natural colorants. The objective of this work was to evaluate the influence of some factors on natural colorants production by a recently isolated from Amazon Forest, Penicillium purpurogenum DPUA 1275 employing statistical tools. To this purpose the following variables: orbital stirring speed, pH, temperature, sucrose and yeast extract concentrations and incubation time were studied through two fractional factorial, one full factorial and a central composite factorial designs. The regression analysis pointed out that sucrose and yeast extract concentrations were the variables that influenced more in colorants production. Under the best conditions (yeast extract concentration around 10 g/L and sucrose concentration of 50 g/L) an increase of 10, 33 and 23% respectively to yellow, orange and red colorants absorbance was achieved. These results show that P. purpurogenum is an alternative colorants producer and the production of these biocompounds can be improved employing statistical tool.
Descrição
Palavras-chave
natural colorants, filamentous fungi, optimization, submerged culture
Como citar
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 45, n. 2, p. 731-742, 2014.