STORAGE RESULTS IN LOSS of THE ANTIGENOTOXIC PROPERTIES of LENTINULA EDODES (SHIITAKE MUSHROOM) and DEVELOPMENT of IN VIVO GENOTOXICITY
Nenhuma Miniatura disponível
Data
2010-02-01
Autores
Alves de Lima, Patricia L. [UNESP]
Ribeiro, Daniel A.
Sugui, Marina M. [UNESP]
Terezan, Ana Paula
Domingues, Maria Aparecida Custódio [UNESP]
Filho, E. R.
Azevedo, Luciana
Salvadori, Daisy Maria Favero [UNESP]
Ribeiro, Lúcia Regina [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell Publishing, Inc
Resumo
In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at -20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. on day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. The mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. The results indicated that storage at both room temperature and at -20C significantly reduced the antigenotoxic effects of the mushroom powder. In addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at -20C resulted in genotoxic activity (P < 0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity.
Descrição
Palavras-chave
Como citar
Journal of Food Biochemistry. Malden: Wiley-blackwell Publishing, Inc, v. 34, n. 1, p. 206-228, 2010.