(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: structural characterization and pro-inflammatory activity
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Data
2015-11-20
Autores
Oliveira, Kassandra S. M. [UNESP]
Di Bastiani, Mirela [UNESP]
Cordeiro, Lucimara M. C.
Costa, Mírian F. [UNESP]
Toledo, Karina A. [UNESP]
Iacomini, Marcello
Babosa, Aneli M.
Dekker, Robert F. H.
Nascimento, Valéria M. G. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B. V.
Resumo
The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.
Descrição
Palavras-chave
Lasiodiplodan, Linear (1→6)--glucan, (1→3)(1→6)--Glucan, Exopolysaccharides, Immunomodulatory activity
Como citar
Carbohydrate Polymers, v. 133, p. 539-546, 2015.