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dc.contributor.authorBraz, Jaqueline Derissi [UNESP]
dc.contributor.authorPitangui, Nayla de Souza [UNESP]
dc.contributor.authorYamashita, Caetano Toshiro [UNESP]
dc.contributor.authorAlmeida, Ana Marisa Fusco [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorSardi, Janaína de Cássia Orlandi [UNESP]
dc.date.accessioned2016-01-28T16:56:17Z
dc.date.available2016-01-28T16:56:17Z
dc.date.issued2014
dc.identifierhttp://www.pakinsight.com/pdf-files/IJMHSR-2014-1(10)-119-125.pdf
dc.identifier.citationInternational Journal of Medical and Health Sciences Research, v. 1, n. 10, p. 119-125, 2014.
dc.identifier.issn2277-4505
dc.identifier.urihttp://hdl.handle.net/11449/133714
dc.description.abstractCandida yeasts are common in the oral cavity and can cause candidosis in the presence of predisposing factors, especially diabetes. The manifestation of the disease is related to this set of local factors such as the presence of dental prostheses, salivary pH, salivary flow and tobacco and the ability to form biofilms. Biofilms are specific and organized communities of cells under the control of signaling molecules rather than random accumulations of cells resulting from cell division and frequently are drugs resistance. Aim: The objectives of this study were to determine the genetic patterns of these C. albicans isolates and to evaluate the in vitro activity amphotericin B and caspofungin against C. albicans biofilms. Methods: Microbial samples were collected from subgingival sites and seeded in CHROMagar for subsequent identification of C. albicans by PCR. Genotypes were defined based on the identification of the transposable introns in the 25S rDNA by PCR. Results: In this study, 6 strains were identified as C. albicans and of these, 3 strains were genotype A and 3 were genotype B. The results showed that both amphotericin B and caspofungin exhibited strong antifungal activities against C. albicans biofilm formation and inhibiting the biofilm formation ranging from 70.8 – 95.3% and 77.7 - 88.7%, respectively. The antifungals studied had low inhibitory effect on preformed biofims, ranging from 39.5 - 50.8% for amphotericin B and from 23.1 - 36.9% for caspofungin at the same concentration. The activity of the two drugs was most effective in inhibit biofilm formation.en
dc.format.extent119-125
dc.language.isoeng
dc.relation.ispartofInternational Journal of Medical and Health Sciences Researc
dc.sourceCurrículo Lattes
dc.subjectC. albicansen
dc.subjectIdentificationen
dc.subjectBiofilmen
dc.subjectCaspofunginen
dc.subjectAmphotericin Ben
dc.subjectResistanceen
dc.titleGenetic evaluation and activity of antifungal against clinical isolate Candida albicans biofilmsen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, R. Expedicionários do Brasil, 1621 - Laboratório de Micologia Clínica, Centro, CEP 14801902, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, R. Expedicionários do Brasil, 1621 - Laboratório de Micologia Clínica, Centro, CEP 14801902, SP, Brasil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.lattes3716273524139678
dc.identifier.lattes8972540207861201
dc.identifier.lattes9458159592579237
unesp.departmentAnálises Clínicaspt
dc.identifier.orcid0000-0002-8059-0826
unesp.author.lattes3716273524139678
unesp.author.lattes8972540207861201
unesp.author.lattes9458159592579237
unesp.author.orcid0000-0002-8059-0826[5]
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