Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ

URI Permanente para esta coleção

Navegar

Submissões Recentes

Agora exibindo 1 - 20 de 696
  • ItemArtigo
    Applicability of the drainage effectiveness of Blake drains and Tenckhoff catheters in the peritoneal dialysis of healthy rabbits (Oryctolagus cuniculus)
    (2022-11-01) Pereira, Ana Camila [UNESP]; Geraldes, Silvano S. [UNESP]; Maia, Suellen R. [UNESP]; de Azevedo, Maria Gabriela P. [UNESP]; da Silva, Gustavo G. [UNESP]; de Souza, Bruna N. [UNESP]; García, Henry David M. [UNESP]; Melchert, Alessandra [UNESP]; Brandão, Cláudia Valéria S. [UNESP]; de Souza, Fabiana F. [UNESP]; Guimarães-Okamoto, Priscylla T. C. [UNESP]; Universidade Estadual Paulista (UNESP)
    Objective: To compare the efficacy of dialysate drainage between Tenckhoff (TC) catheter and Blake (BL) in peritoneal dialysis (PD) in healthy rabbits. Design: Prospective experimental study. Setting: University Teaching Hospital and University research laboratory. Animals: Twenty healthy, male, New Zealand rabbits. Interventions: PD via the TC catheter and the BL abdominal drain was compared during 3 consecutive days of dialysis delivery. Measurements and Main Results: One session of PD was performed per day for 3 consecutive days (S1: first session, S2: second session, S3: third session) and each session included 4 cycles of infusion, dwell of dialysate in the abdomen, and drainage. Data collection included daily urea, creatinine, alanine aminotransferase, albumin, and potassium, in addition to hematological parameters (eg, RBC, HCT, hemoglobin, WBC, and platelet count). Statistical analysis using a mixed linear model with multiple comparisons was performed. The BL drain resulted in an increase in volume drained (ml/kg) when compared to TC catheter on S2 (third and fourth cycles) and S3 (first and second cycles). Conclusions: The BL drain proved to be superior to the TC catheter, being capable of draining a larger volume of dialysate during the drainage processes in the peritoneal PD of healthy rabbits. The TC catheter had major complications with regard to fluid retention in the abdomen, representing reduced drainage efficiency, while the BL drain showed a greater tendency for the peritoneal fluid to leak.
  • ItemArtigo
    Stem Cell Therapy for Veterinary Orthopedic Lesions
    (Wiley-Blackwell, 2014-01-01) Balesdent Barreira, Anna Paula; Garcia Alves, Ana Liz [UNESP]; Schatten, H.; Univ Fed Rural Rio de Janeiro; Universidade Estadual Paulista (UNESP)
  • ItemResumo
    CANINE MESENCHYMAL STEM CELLS CULTURED WITH DESFERRIOXAMINE (DFO) AND INTERFERON GAMMA TO MIMICKING HYPOXIA AND INFLAMMATION CONDITIONS
    (Elsevier B.V., 2022-10-01) Landim, F. C. [UNESP]; Ortiz, P. E. O. [UNESP]; Aristizabal, V. H. V. [UNESP]; Stuart, J. B. A. P. [UNESP]; Dellaqua, T. T. [UNESP]; Universidade Estadual Paulista (UNESP); Omics Biotecnol Anim Ltda
  • ItemArtigo
    Postoperative Acupuncture is as Effective as Preoperative Acupuncture or Meloxicam in Dogs Undergoing Ovariohysterectomy: a Blind Randomized Study
    (2022-01-01) Ferro, Ana Carla Zago Basilio [UNESP]; Cannolas, Caroline; Reginato, Juliana Cristianini; Luna, Stelio Pacca Loureiro [UNESP]; Universidade Estadual Paulista (UNESP); Jaú Pet Care
    Background: Acupuncture has the same analgesic effect as non-steroidal antiinflammatory drugs and opioids. It is challenging to perform preoperative acupuncture in unmanageable animals, while the residual postoperative anesthetic effect facilitates the performance of acupuncture postoperatively. Objectives: To compare preoperative acupuncture or meloxicam versus postoperative acupuncture for postoperative analgesia after ovariohysterectomy. Methods: This is a horizontal prospective positive control blind randomized experimental study. Thirty-six dogs were randomly divided into three groups: GA (preemptive acupuncture), GPA (postoperative acupuncture), and GM (meloxicam 0.2 mg/kg IV preoperatively). After sedation with acepromazine (0.05 mg/kg IM), anesthesia was induced with propofol (5.3 ± 0.3 mg/kg) and maintained with isoflurane/O2. Fentanyl (2 μg/ kg, IV) was administered immediately before surgery. Bilateral acupuncture was performed at acupoints Large intestine 4, Spleen 6, and Stomach 36 for 20 minutes, before (GA) or immediately after surgery (GPA). Pain was evaluated by an observer blind to the treatment using the Glasgow scale before and for 24 hours after ovariohysterectomy. Dogs with a score ≥ 6 received rescue analgesia with morphine (0.5 mg/kg IM). Nonparametric data were analyzed by the Kruskal–Wallis test, followed by Dunn’s test and parametric data by ANOVA followed by Tukey’s test. Results: Two GA and one GPA dogs received rescue analgesia once. Two GM dogs received rescue analgesia and one of those was treated again twice. There were no differences in the number of dogs receiving rescue analgesia between groups (p = 0.80). Conclusion: Postoperative acupuncture was as effective as preoperative acupuncture or meloxicam in female dogs undergoing ovariohysterectomy.
  • ItemArtigo
    Seroprevalence to Rabies Virus in Wildlife in Brazil
    (2022-04-01) Fornazari, Felipe [UNESP]; Scheffer, Karin Corrêa; Dos Ramos Silva, Sandriana; da Silva, Karina Ribeiro; Rodrigues, Adriana Cândido; Teixeira, Carlos Roberto [UNESP]; Rolim, Luna Scarpari [UNESP]; Langoni, Helio [UNESP]; Universidade Estadual Paulista (UNESP)
    Serum samples of 638 free-ranging wild mammals from São Paulo state, Brazil, were tested for neutralizing antibodies against rabies virus by the rapid fluorescent focus inhibition test. Overall seroprevalence was 1.7% among 24 species surveyed, with individuals of six species having positive results indicating exposure to rabies virus.
  • ItemArtigo
    Short-term effects of Whole-Body Vibration on clinical, cardiac, and ambulatory electrocardiographic (Holter) parameters of healthy younger and older adult male non-athletic crossbreed dogs
    (2022-01-01) Silva, B. M. [UNESP]; Rahal, S. C. [UNESP]; Filippi, M. G. [UNESP]; Aleixo, A. S.C. [UNESP]; Codognoto, V. [UNESP]; Tsunemi, M.; Viegas, C. A.A.; Santos, I. F.C. [UNESP]; Universidade Estadual Paulista (UNESP); Instituto de Biociências de Botucatu; Escola de Ciências Agrárias e Veterinárias
    This study investigated the effects of WBV in clinical, cardiac, and ambulatory electrocardiographic (Holter) parameters of healthy young and aged male non-athletic dogs. Fourteen dogs were divided into two groups of seven animals: Group I (GI) – young dogs (12.0 and 84.0 months old); Group II (GII) – aged dogs (above 84.0 months old). Dogs were submitted to a single session of WBV (frequencies of 30 and 50 Hz), for 15-min. Variations were identified in the thickness of the interventricular septum and thickness of the left ventricle-free wall values: GI < GII at 5-min before the session. The diameter of the left atrium values showed a difference: GI < GII at 5-min before and 1-min after the session; and a decrease in GII between 5-min before and 1 min after the WBV. Several ambulatory electrocardiography (Holter) parameters demonstrated significant differences between both groups and time-points. A single session of WBV at frequencies of 30 and 50 Hz during 15-min by using a vibrating platform that delivered a vortex wave circulation did not induce significant changes in clinical, cardiac, and ambulatory electrocardiographic (Holter) parameters in healthy young and aged dogs
  • ItemArtigo
    Effects of Acupuncture in the Treatment of Dogs with Neurological Sequels of Distemper Virus
    (2022-01-01) Santos, Bianca P. C. R. [UNESP]; Joaquim, Jean G. F.; Cassu, Renata Navarro; Pantoja, José C. F. [UNESP]; Luna, Stelio Pacca Loureiro [UNESP]; Universidade Estadual Paulista (UNESP); Bioethicus Institute; University of Western São Paulo (Unoeste)
    Background: Acupuncture (AP) has been empirically used to relieve post-canine distemper virus (CDV) infection neurological signs in veterinary clinics. Objectives: This clinical study aimed to investigate the effects of AP combined with electroacupuncture (EA) on neurological function in dogs infected by CDV. Methods: Twenty-four CDV-infected dogs with neurological sequelae were recruited to receive weekly AP/EA sections for 24 weeks. Neurological improvements were assessed before each AP/EA session using a modified scoring system. Data were analyzed using the McNemar test, Friedman test, Fisher's exact test, and Kaplan-Meier curves (p < 0.05). Results: Neurological scores improved from seven to 24 weeks after AP/EA treatment compared with pretreatment scores (p < 0.001). Significant improvements were recorded over time for functional limb recovery, cranial nerve deficits, mental status (p = 0.025 – 0.014), and urinary function (p < 0.001). Myoclonus was improved and entirely reversed in 75% and 25% of the dogs, respectively. At the end of treatment, the proportion of dogs with normal proprioception, posture, hopping (p < 0.001), and superficial pain sensation responses (p = 0.004) was greater than pretreatment values. Conclusion: AP/EA therapy promoted significant neurological recovery in CDV-infected dogs and may be considered within the chronic phase of the disease to improve motor and sensory rehabilitation. However, these results are preliminary and must be confirmed by further investigations.
  • ItemErrata
    Sem título
    (2022-07-01) Stijn, Schauvliege; Charlotte, Cuypers; Anneleen, Michielsen; Frank, Gasthuys; Miguel, Gozalo-Marcilla [UNESP]; Ghent University; Universidade Estadual Paulista (UNESP)
    The authors regret that an error was included in their published paper. In the legend of Table 3 on p.10 and in the last paragraph of the results on p. 11, the administration rate for detomidine was wrongly expressed per minute, this should have been per hour. • In the legend of Table 3, it is wrongly stated ‘for detomidine, changes by 2.5 μg kg-1 min-1 are proposed’ and ‘for detomidine, changes by 5 μg kg-1 min-1 are proposed’. These should respectively read ‘for detomidine, changes by 2.5 μg kg-1 hour-1 are proposed’ and ‘for detomidine, changes by 5 μg kg-1 hour-1 are proposed’. • In the last paragraph of the results, it is wrongly stated ‘for detomidine, changes in the rate by 2.5 μg kg-1 minute-1 are proposed, since the initial rate of detomidine infusion for standing surgical procedures of the authors is usually around 10 μg kg-1 minute-1’. This should read: ‘for detomidine, changes in the rate by 2.5 μg kg-1 hour-1 are proposed, since the initial rate of detomidine infusion for standing surgical procedures of the authors is usually around 10 μg kg-1 hour-1’. The authors apologize for any inconvenience caused.
  • ItemResenha
    Comparative Pathobiology of Canine and Human Prostate Cancer: State of the Art and Future Directions
    (2022-06-01) Nascente, Eduardo de Paula; Amorim, Renée Laufer [UNESP]; Fonseca-Alves, Carlos Eduardo [UNESP]; de Moura, Veridiana Maria Brianezi Dignani; Universidade Federal de Goiás (UFG); Universidade Estadual Paulista (UNESP)
    First described in 1817, prostate cancer is considered a complex neoplastic entity, and one of the main causes of death in men in the western world. In dogs, prostatic carcinoma (PC) exhibits undifferentiated morphology with different phenotypes, is hormonally independent of aggressive character, and has high rates of metastasis to different organs. Although in humans, the risk factors for tumor development are known, in dogs, this scenario is still unclear, especially regarding castration. Therefore, with the advent of molecular biology, studies were and are carried out with the aim of identifying the main molecular mechanisms and signaling pathways involved in the carcinogenesis and progression of canine PC, aiming to identify potential biomarkers for diagnosis, prognosis, and targeted treatment. However, there are extensive gaps to be filled, especially when considering the dog as experimental model for the study of this neoplasm in humans. Thus, due to the complexity of the subject, the objective of this review is to present the main pathobiological aspects of canine PC from a comparative point of view to the same neoplasm in the human species, addressing the historical context and current understanding in the scientific field.
  • ItemArtigo
    Influence of the Near-Cortical Over-Drilling Technique on the Mechanical Behaviour of Locking Plate Constructs Applied in Maned Wolf's Femur
    (2022-01-01) Siqueira, Rafael C. [UNESP]; Rahal, Sheila C. [UNESP]; Mesquita, Luciana R. [UNESP]; Voorwald, Fabiana A.; Fernandes, Martin F. [UNESP]; Tosati, Marcos; Ribeiro, Celso R.; Scorsato, Paulo S.; Universidade Estadual Paulista (UNESP); Federal University of Viçosa; SAMT - Structural Solutions for Finite Elements; Mechanical and Metallography Testing Laboratory - LEMM; University of Marília (UNIMAR)
    Objective The aim of this study was to evaluate the influence of near-cortical over-drilling holes on the mechanical behaviour of locking plate constructs applied in maned wolf's femur by using mechanical testing and finite element method (FEM). Study Design Seven pairs of adult maned wolves (Chrysocyon brachyurus) femur bones were randomly distributed into four groups. In all groups, a 3.5 mm locking compression plate, designed with 12 combi-holes and one locked, was applied to the lateral surface of the femur. G1 (n = 4) received bicortical locking screws placed in holes 1, 3, 5, 8, 10 and 12. In G2 (n = 5), the plate was applied as used in G1, but the application of the locked screws involved the near-cortical over-drilling technique. In G3 (n = 4), the plate was applied as used in G2, but the size of the near-cortical over-drilling was larger. The combi-holes 6 and 7 were maintained over a 10 mm fracture gap without screws. All constructs were tested for failure in the axial load. The axial load was applied eccentrically to the femoral head. Results Statistical differences were observed in the maximum load with G3 > G1 and G3 > G2, and in the deflection with G2 > G1 and G2 > G3. The FEM showed the lowest total displacement of the bone-plate constructs as well as of the plate in G1 compared with G2 and G3. Conclusion The near-cortical over-drilling technique used in unstable fractures induced in the maned wolf's femur showed by static axial compression test that maximum load and deflection are dependent on drill hole size induced in the near-cortex. Based on FEM, the lowest total displacement of the bone-plate constructs was observed in Group 1.
  • ItemArtigo
    Cardiovascular and Gas Exchange Effects of Individualized Positive End-Expiratory Pressures in Cats Anesthetized With Isoflurane
    (2022-05-04) Machado, Marcela L.; Soares, Joao H. N.; Pypendop, Bruno H.; Aguiar, Antonio J. A. [UNESP]; Braun, Christina; Motta-Ribeiro, Gabriel C.; Jandre, Frederico C.; Davis; Universidade Estadual Paulista (UNESP); University of Veterinary Medicine Vienna; Federal University of Rio de Janeiro
    Objectives: To compare the effects of four levels of end-expiratory pressure [zero (ZEEP) and three levels of positive end-expiratory pressure (PEEP)] on the cardiovascular system and gas exchange of cats anesthetized with isoflurane and mechanically ventilated for 3 h with a tidal volume of 10 ml/kg. Study Design: Prospective, randomized, controlled trial. Animals: Six healthy male neutered purpose-bred cats. Methods: Anesthesia was induced with isoflurane and maintained at 1.3 minimum alveolar concentration. PEEP of maximal respiratory compliance (PEEPmaxCrs) was identified in a decremental PEEP titration, and cats were randomly ventilated for 3 h with one of the following end-expiratory pressures: ZEEP, PEEPmaxCrs minus 2 cmH2O (PEEPmaxCrs−2), PEEPmaxCrs, and PEEPmaxCrs plus 2 cmH2O (PEEPmaxCrs+2). Cardiovascular and gas exchange variables were recorded at 5, 30, 60, 120, and 180 min (T5 to T180, respectively) of ventilation and compared between and within ventilation treatments with mixed-model ANOVA followed by Dunnet's and Tukey's tests (normal distribution) or Friedman test followed by the Dunn's test (non-normal distribution). Significance to reject the null hypothesis was considered p < 0.05. Results: Mean arterial pressure (MAP—mmHg) was lower in PEEPmaxCrs+2 [63 (49–69); median (range)] when compared to ZEEP [71 (67–113)] at T5 and stroke index (ml/beat/kg) was lower in PEEPmaxCrs+2 (0.70 ± 0.20; mean ± SD) than in ZEEP (0.90 ± 0.20) at T60. Cardiac index, oxygen delivery index (DO2I), systemic vascular resistance index, and shunt fraction were not significantly different between treatments. The ratio between arterial partial pressure and inspired concentration of oxygen (PaO2/FIO2) was lower in ZEEP than in the PEEP treatments at various time points. At T180, DO2I was higher when compared to T5 in PEEPmaxCrs. Dopamine was required to maintain MAP higher than 60 mmHg in one cat during PEEPmaxCrs and in three cats during PEEPmaxCrs+2. Conclusion: In cats anesthetized with isoflurane and mechanically ventilated for 3 h, all levels of PEEP mildly improved gas exchange with no significant difference in DO2I when compared to ZEEP. The PEEP levels higher than PEEPmaxCrs−2 caused more cardiovascular depression, and dopamine was an effective treatment. A temporal increase in DO2I was observed in the cats ventilated with PEEPmaxCrs. The effects of these levels of PEEP on respiratory mechanics, ventilation-induced lung injury, as well as in obese and critically ill cats deserve future investigation for a better understanding of the clinical use of PEEP in this species.
  • ItemArtigo
    Pathology in Practice
    (2022-05-20) Dinau, Fernando C. [UNESP]; Ghedin, Victoria [UNESP]; Moura, Fernanda B. C. de [UNESP]; Ferreira, Mayara F. [UNESP]; Machado, Eduardo F.; Rocha, Noeme S. [UNESP]; Watanabe, Tatiane Terumi Negrão; Universidade Estadual Paulista (UNESP); Adolfo Lutz Institute; Universidade de São Paulo (USP); North Carolina State University
  • ItemArtigo
    Open Fracture of the Femur in a Two-toed Sloth (Choloepus didactylus) - Treatment and Rehabilitation
    (Univ Fed Rio Grande Do Sul, 2022-01-01) Santos, Laynara Silva dos [UNESP]; Rahal, Sheila Canevese [UNESP]; Lima, Natalia Aparecida de Souza; Cunha, Daniel Alexander Pereira da; Nava, Alessandra Ferreira Dales; Chiesorin Neto, Laerzio; Teixeira, Carlos Roberto [UNESP]; Universidade Estadual Paulista (UNESP); Inst Brasileiro Meio Ambiente & Recursos Renovave; Ctr Med & Clin Vet Minas Pet CVMinasPet; Fiocruz Amazonia
    Background: The Choloepus didactylus is characterized by having 2 fingers on the forelimbs and 3 on the hind limbs, being found more frequently in northern South America, in countries such as Venezuela, Guyana, Ecuador, Peru, and Brazil. In Brazil, deforestation of the Amazon rainforest has threatened the survival of C. didactylus. In addition, these animals can be affected by several diseases, being those of the musculoskeletal system with limited reports. Thus, the present report aimed to describe the treatment and evolution of an open fracture of the femur in a free-living Choloepus didactylus, which after rehabilitation was released back to the wild. Case: A free-living female two-toed sloth (Choloepus didactylus), weighing 7.0 kg, was found in Manaus city and referred to the Wild Animal Treatment Center (CETAS - IBAMA-AM) located in Amazonas, Brazil, after initial treatment and osteosynthesis in a private clinic (CVMinasPet). According to history, the animal had suffered electrocution 30 days ago and, as a result, had an extensive wound that resulted in the exposure of the knee joint, distal fracture of the femur, and areas of necrosis in the right pelvic limb. After cleansing of the area and removal of necrotic tissues, the fracture was stabilized with the cross-pinning technique (2 Steinmann pins). Then, the stifle joint was stabilized with external skeletal fixation (Type 1b, unilateral biplanar). The external fixator and cross-pins were removed 2 months after the surgical procedure, being observed bone healing. Next, the animal underwent physical rehabilitation for 30 days. After the rehabilitation period, a microchip and a radio collar were applied, and the sloth was released back to the wild. Discussion: Electrocution has been one of the causes of wildlife rescue, with consequences varying depending on the degree of the burn. Generally, the burn is most severe at the entry and exit sites. Since suspensory quadrupedal locomotion requires that sloths have specialized hands and feet to connect with the supports, probably the animal of the current report touched the right pelvic limb on a high-tension pole. The cross-pinning technique combined with the external fixator was used because the animal had an exposed Salter-Harris type I fracture with soft tissue loss. The younger the animal presents physeal fractures, the greater is the chance of developing growth abnormalities due to growth plate closure caused by fracture type or immobilization method. Since the sloth was a free-living animal, the age was unknown. However, pelvic limb length discrepancy was not observed after removing the implants. Culture and antimicrobial susceptibility test were not done and may be considered a limitation of this report. However, the application of ceftriaxone was adequate to control the infection, since the animal did not show signs of infection or draining sinus tracts. Ceftriaxone is a third-generation antibiotic that can be used in chronic osteomyelitis. In addition, the animal in the present report showed good adaptation to captive conditions that contributed to the clinical management. The longevity of the sloth in captivity can reach more than 30 years, but the goal is always to return the animal to nature after the treatment. Surgical treatment of the fracture and physical therapy after implant removal allowed this action in the current report, confirmed by monitoring with the radio collar.
  • ItemResumo
    Tendon derived extracellular matrix hydrogel for injection and bioprinting
    (Mary Ann Liebert, Inc, 2022-04-01) Alves, Ana Liz Garcia [UNESP]; Stievani, Fernanda de Castro [UNESP]; Gomez-Florit, Manuel; Bakht, Syeda Mahwish; Pfeifer, Joao Pedro Hubbe [UNESP]; Appolonio, Emanuel Vitor Pereira [UNESP]; Rossi, Mariana Correa [UNESP]; Rosa, Gustavo dos Santos [UNESP]; Domingues, Rui; Gomes, Manuela Estima; Universidade Estadual Paulista (UNESP); Univ Minho
  • ItemArtigo
    Effects of Reticulated Hyaluronic Acid Alone or Associated With Whole-Body Vibration in Dogs With Osteoarthritis Due to Hip Dysplasia
    (2022-07-01) Martins, David J.C. [UNESP]; Rahal, Sheila C. [UNESP]; Silva Júnior, José I.C. [UNESP]; Michelon, Fernanda [UNESP]; Tomacheuski, Rúbia M. [UNESP]; Correia, Luiz E.C.S. [UNESP]; Woods, Lia S. [UNESP]; Mamprim, Maria J. [UNESP]; Lourenço, Maria L.G. [UNESP]; Santos, Ivan F.C. [UNESP]; Universidade Estadual Paulista (UNESP)
    This study aimed to evaluate the effects of reticulated hyaluronic acid (HA) alone or associated with whole-body vibration (WBV) in dogs with osteoarthritis due to hip dysplasia. Fourteen dogs were randomized assigned into 2 groups of 7 animals: Group 1 (G1) — single intra-articular injection of hyaluronic acid; Group 2 (G2) — single intra-articular injection of hyaluronic acid associated with WBV sessions. The affected hip joint received 0.70 mL of reticulated HA guided by ultrasound. Dogs were submitted to a single session of WBV (30 and 50 Hz, for 15 min) every 48 hours for 12 weeks. Dogs were evaluated for morphometric measurements; orthopedic, radiographic and lameness scores of the hip joints; kinetic analysis; and ultrassonographic measurement of the following muscles: middle gluteal, vastus lateralis and biceps femoris. The morphometric measurements, lameness scores, and muscle measurements were conducted at 10 minutes before treatments (TP0), and at days 30 (TP30), 60 (TP60) and 90 (TP90) after treatments. The orthopedic and radiographic scores and kinetic analysis were performed at TP0 and TP90. The scores of lameness showed a statistical decrease in G1 and G2 between time-points. Significant decreases (P =.01) were observed in orthopedic scores in both groups between time-points. The Peak Vertical Force between TP0 and TP90 was significantly higher in G2 (P =.01). Vertical Impulse was null in G1 and positive in G2. Dogs treated with single intra-articular injection of hyaluronic acid alone and associated with WBV had beneficial effects in dogs with osteoarthritis due to hip dysplasia, however the association of viscosupplementation with hyaluronic acid and WBV had an earlier improvement clinical outcome and allowed better kinetic results.
  • ItemArtigo
    Anesthetic Pharmacology of the Mint Extracts L-Carvone and Methyl Salicylate
    (2022-01-01) Brosnan, Robert J.; Ramos, Kimberly; Aguiar, Antonio Jose De Araujo [UNESP]; Cenani, Alessia; Knych, Heather K.; University of California; Universidade Estadual Paulista (UNESP)
    Introduction: Hydrocarbons with sufficient water solubility allosterically modulate anesthetic-sensitive ion channels. Mint extracts L-carvone and methyl salicylate water solubility exceeds modulation cutoff values for γ-amino butyric acid type A (GABAA) receptors, N-methyl-D-aspartate (NMDA) receptors, and type-2 voltage-gated sodium (Nav1.2) channels. We hypothesized that mint extracts modulate these channels at concentrations that anesthetize rats. Methods: Channels were expressed separately in frog oocytes and studied using 2-electrode voltage clamp techniques at drug concentrations up to 10 mM. Normalized current effects were fit to Hill equations. Mint compounds were formulated in a lipid emulsion and administered IV to rats. When unresponsive to the tail clamp, rats were exsanguinated, and plasma drug concentrations were measured. Results: Both mint compounds caused concentration-dependent inhibition of all channels except for methyl salicylate which inhibited GABAA receptors at low concentrations and potentiated at high concentrations. Plasma drug concentrations in anesthetized rats were 7.9 mM for L-carvone and 2.7 mM for methyl salicylate. This corresponded to ≥53% NMDA receptor inhibition and ≥78% Nav1.2 channel inhibition by both compounds and 30% potentiation of GABAA receptors by methyl salicylate. Conclusion: L-Carvone and methyl salicylate allosterically modulate cell receptor targets important to molecular actions of conventional anesthetics at concentrations that also induce general anesthesia in rats.
  • ItemArtigo
    Beta-caryophyllene as an antioxidant, anti-inflammatory and re-epithelialization activities in a rat skin wound excision model
    (2022-01-01) Gushiken, Lucas Fernando Sérgio [UNESP]; Beserra, Fernando Pereira [UNESP]; Hussni, Maria Fernanda [UNESP]; Gonzaga, Murilo Tireli [UNESP]; Ribeiro, Victor Pena; De Souza, Patrícia Fernanda; Campos, Jacqueline Costa Lima; Massaro, Taís Nader Chrysostomo; Hussni, Carlos Alberto [UNESP]; Takahira, Regina Kiomi [UNESP]; Marcato, Priscyla Daniely; Bastos, Jairo Kenupp; Pellizzon, Cláudia Helena [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
    The skin is a critical organ for the maintenance of the integrity and protection of the organism. When a wound occurs, a sequence of healing mechanisms is triggered to reconstruct the wounded area. β-caryophyllene is a sesquiterpene in Copaifera langsdorffii oleoresin with antioxidant and anti-inflammatory potential. On the basis of previous studies with C. langsdorffii, β-caryophyllene was selected to evaluate its wound healing potential and pharmacological mechanisms. The excision wound model was used with male Wistar rats and macroscopic, histological, immunohistochemical and biochemical analyses were performed with skin samples, comparing the β-caryophyllene-Treated group with reference drugs. The results showed macroscopic retraction of the wounds treated with β-caryophyllene. Biochemical assays revealed the antioxidant and anti-inflammatory mechanisms of the β-caryophyllene-Treated group with increasing levels of IL-10 and GPx and decreasing levels of pro-inflammatory molecules, including TNF-α, IFN-γ, IL-1β and IL-6. After β-caryophyllene treatment, immunohistochemical assays showed enhanced re-epithelialization, through the increase in laminin-γ2 and desmoglein-3 immunolabeling. β-caryophyllene also act in the remodeling mechanism, increasing the collagen content in the Masson's trichrome staining. These findings indicated the wound-healing potential of β-caryophyllene topical formulation in rat skin wounds, mediated by antioxidant, anti-inflammatory and re-epithelialization mechanisms.
  • ItemArtigo
    Combination of Autohemotherapy and Vincristine Sulfate in Treatment of Canine Transmissible Venereal Tumor in Bitches in Mozambique
    (2021-01-01) Júnior, Fernando Chissico; Bambo, Otilia Rafael; da Mota Cardoso, José Manuel; Laisse, Cláudio João Mourão; Gallina, Marina Frazatti [UNESP]; Zadra, Vivian Ferreira; Rahal, Sheila Canevese [UNESP]; dos Santos, Ivan Felismino Charas [UNESP]; Universidade Estadual de Maringá (UEM); Universidade Estadual Paulista (UNESP); Bandeirantes (CLM)
    Background: Canine transmissible venereal tumor (TVT) is a tumor of round cells. Vincristine sulfate is the most effective for TVT. Alternatively, hemotherapy is an alternative therapy that consists of the administration of autologous blood and the positive effects are associated with an immunomodulatory effect. Since chemotherapy has some collateral effects, it is necessary to study another treatment with minimal side effects. In this context, this report case aimed to describe the use of autohemotherapy associated with vincristine sulfate for treating a transmissible venereal tumor in the vulvar mucosa of 7 adult bitches, being the first case report in Mozambique, Africa. Case: Seven adult bitches, median size, were referred to the School Veterinary Hospital, School of Veterinary, Eduardo Mondlane University, Maputo, Mozambique, Africa, with a diagnosis of TVT in the vulvar mucosa. All bitches were treated weekly with autohemotherapy and vincristine sulfate for 21 days. The parameters assessed included clinical and TVT macroscopic examination, complete blood count, serum biochemical examination and urinalysis, and were evaluated 60-min before each treatment. No clinical side effects were identified during the treatments. Color, appearance and tumor size were changed during the treatment period, and all bitches showed complete remission of the tumor 21 days after the beginning of treatment or after the third therapeutic session. The values of the complete blood count, serum biochemical and urinalysis did not demonstrate significant variations throughout the evaluated time-points. The TVT cytopathological classification was lymphocytic (42.9 %), plasmacytic (28.6 %) and lymphoplasmacytic (28.6 %). Discussion: The aims of this report were to describe the combination of autohemotherapy and vincristine sulfate for treating the transmissible venereal tumor located in the vulvar mucosa of adult bitches, through clinical and laboratory evaluation, and was not identified side and significant hematological changes. The novelty of this case report was associated with the use of adult bitches with TVT in the vulvar mucosa. Other authors conducted a similar study however with male dog with TVT identified at the base of the penis. The complete remission of the tumor after 3 applications and the absence of side effects showed the effectiveness of this treatment compared with use of chemotherapy without autohemotherapy. In contrast, dogs diagnosed with TVT and treated with vincristine sulfate showed complete remission after 4 treatment sessions However, the use of autohemotherapy alone for treating extragenital TVT in bitches did not induce complete remission after 6 weekly treatments. Doses and administration of autologous blood and vincristine sulfate were in accordance with the recommendations of the literature, and were determined according to body mass. Other routes of administration through the cephalic vein were also used and showed complete remission after 4 treatments. The decrease in size tumor associated with the changes in appearance and color was associated with regression of the TVT. The connective tissue isolating groups of cells identified in the literature were not confirmed in this report, probably due to the phase of the progression of the tumor. The highest percentage of animals with lymphocytoid TVT was different from the literature, which referred to the predominance of the plasmacytoid pattern. The combination of autohemotherapy and vincristine sulfate every 7 days encouraged complete remission of TVT in the vulvar mucosa of adult median size bitches after 3 sessions.
  • ItemArtigo
    Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
    (2021-12-01) da Silva Dias, Carolina [UNESP]; Rossi, Mariana Correa [UNESP]; Apolonio, Emanuel V. P. [UNESP]; dos Santos Rosa, Gustavo [UNESP]; Pfeifer, João Pedro Hübbe [UNESP]; Hussni, Carlos Alberto [UNESP]; Watanabe, Marcos Jun [UNESP]; Alves, Ana Liz Garcia [UNESP]; Universidade Estadual Paulista (UNESP)
    Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release. [Figure not available: see fulltext.]
  • ItemArtigo
    Treatment of chronic spinal cord injury in dogs using amniotic membrane-derived stem cells: Preliminary results
    (2021-01-01) Orlandin, Jéssica Rodrigues; Gomes, Ingrid da Silva; Leandro, Shamira de Fátima Sallum; Cagnim, Artur Fuertes; Casals, Juliana Barbosa; Carregaro, Adriano Bonfim; Freitas, Silvio Henrique; Machado, Luciana Cristina; Castiglioni, Maria Cristina Reis [UNESP]; Alves, Ana Liz Garcia [UNESP]; Machado, Vânia Maria de Vasconcelos [UNESP]; Ambrósio, Carlos Eduardo; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP)
    Introduction: Intervertebral disc diseases (IVDD) represent the majority of neurological attendance and responsible for the most cases of paralysis in dogs. Treatments currently used do not show satisfactory results in patients with more severe and chronic neurological manifestations. Methods: To promote nerve and muscular recovery, as well as improve quality of life, we aimed to create a double-blind test method, associating spinal decompression surgery and allogeneic transplantation of amniotic membrane-derived stem cells (AMSCs) in dogs with chronic IVDD. Cells were characterized as fetal mesenchymal cells and safe for application. Eight animals completed the experiment: stem cell applications were made in four animals that had previously undergone an unsuccessful surgical procedure (“SC group”, n = 4); two animals were submitted to surgery, followed by applications of stem cells (“Surgery + SC”, n = 2); two other animals were submitted to surgery, followed by the application of saline solution (“Surgery + placebo”, n = 2). During the surgical procedure, a topical application was performed on the lesion and after fifteen and forty-five days another two applications were made via epidural. Animals were monitored biweekly and reassessed three months after surgery, by functional tests and magnetic resonance exams. Results: Some animals presented significant neurological improvement, such as the recovery of nociception and ability to remain on station. Despite the need further studies, until the present moment, cell therapy has been feasible and has no harmful effects on animals. Conclusion: The protocol of preclinical trial showed the association with decompressive surgery and cell transplantation in dogs with thoracolumbar IVDD proved feasible, and it was possible to observe neurological improvement after treatment. No tissue improvement through MRI was found. The double-blind test guaranteed reliability of the evaluations and results obtained that, even with a small sample size, generated satisfactory results for the animals and owners.