Leishmania chagasi in opossums (Didelphis albiventris) in an urban area endemic for Visceral Leishmaniasis, Campo Grande, Mato Grosso do Sul, Brazil

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2012

Autores

Humberg, R. M. P.
Oshiro, E. T.
Cruz, M. do S. P. e
Ribolla, Paulo Eduardo Martins [UNESP]
Alonso, Diego Peres
Ferreira, A. M. T.
Bonamigo, R. A.
Tasso, N.
Oliveira, A. G. de

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Resumo

We investigated the occurrence of Leishmania infantum chagasi in Didelphis albiventris opossums at a wild animal rehabilitation center in the city of Campo Grande, Brazil. A total of 54 opossums were tested for L. i. chagasi infection in peripheral blood and bone marrow samples. The samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction–restriction fragment length polymorphism. Leishmania i. chagasi DNA was detected by polymerase chain reaction–restriction fragment length polymorphism in 11 (20.37%) animals. A total of 81.81% of positive opossums were captured in areas of known visceral leishmaniasis transmission. These results suggest a role for D. albiventris in the urban transmission of visceral leishmaniasis.

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Biologia Molecular, Diagnostico molecular, Leishmaniose Visceral

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The American Journal of Tropical Medicine and Hygiene, v. 87, n. 3, p. 470-472, 2012.