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dc.contributor.authorGuastali, Midyan Daroz [UNESP]
dc.contributor.authorMonteiro, Bianca Andriolo [UNESP]
dc.contributor.authorMaziero, Rosiara Rasária D. [UNESP]
dc.contributor.authorPaschoal, Daniela Martins [UNESP]
dc.contributor.authorSudano, Mateus José [UNESP]
dc.contributor.authorRascado, Tatiana da Silva [UNESP]
dc.contributor.authorLima Neto, João Ferreira [UNESP]
dc.contributor.authorVergara, Luis Eduardo [UNESP]
dc.contributor.authorCrocomo, Letícia Ferrari [UNESP]
dc.contributor.authorMaserati, Marc
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz Landim [UNESP]
dc.identifier.citationJournal of Stem Cells, v. 9, n. 4, p. 225-234, 2015.
dc.description.abstractThe objective of this study was to evaluate the potential of bovine IVF blastocysts at different stages of embryonic development in establishing ESC-like. Furthermore, blastocysts cultured in medium containing (10%) and (2.5%) fetal calf serum (FCS) were compared to determine if the serum concentration during in vitro culture alters the blastocyst's potential to establish ESC-like culture. It was observed that only ICM's from expanded blastocysts adhered to the monolayer (n=160) independent of the concentration of serum used during IVF culture. There were no observable differences in potential to establish ESC-like in embryos cultured with 2,5% or 10% FCS . The bFGF didn´t seems to be required for maintenance of bovine ESC-like regardless of culture conditions. Blastocysts and colonies in primary culture and after the first passage were positive for Oct4, Nanog, SSEA-3 and TRA-1-81. Expanded blastocysts gave rise to ESC-like colonies, and the addition of LIF was sufficient to maintain cells undifferentiated in culture.en
dc.relation.ispartofJournal of Stem Cells
dc.sourceCurrículo Lattes
dc.subjectEmbryonic stem cellsen
dc.subjectFetal calf serumen
dc.titleInfluence of culture medium and age of bovine blastocysts in established colonies of embryonic stem cellsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionIn Vitro Brasil Ltda.
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterináriapt
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