Differential alterations in the activity of matrix metalloproteinases within the nervous tissue of dogs in distinct manifestations of visceral leishmaniasis
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Data
2010-08-15
Autores
Machado, Gisele Fabrino [UNESP]
Melo, Guilherme D. [UNESP]
Moraes, Olivia C. [UNESP]
Souza, Milena S. [UNESP]
Marcondes, Mary [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
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Editor
Elsevier B.V.
Resumo
Canine visceral leishmaniasis is an important emerging disease with a multitude of clinical symptoms, including neurological alterations. Matrix metalloproteinases (MMP) are proteases implicated with the extracellular matrix remodelling and, within the central nervous system, these enzymes are involved with blood-brain-barrier disruption and inflammation. To establish the involvement of MMP-2 and -9 within the nervous tissue of dogs with spontaneous visceral leishmaniasis, fragments of nervous tissue from oligosymptomatic (n = 9), symptomatic (n = 8), neurological (n = 12) and normal dogs (n = 8) were subjected to zymographic and to immunohistochemical analysis. Immunohistochemistry evidenced MMP-2 staining in inflammatory cells inside and outside blood vessels. MMP-9 was found in endothelial cells and in the ependyma. Zymographic evaluation revealed only the latent forms of MMP-2 and -9 within the nervous tissue. ProMMP-9 activity in the infected animals was found higher than the normal dogs, but with no difference among the infected dogs. Oligosymptomatic dogs presented the highest proMMP-2 activity, followed by the symptomatic and then, by the neurological and the normal dogs. In summary, the nervous tissue compartment seems to be preserved in dogs with VL, due to the absence of active MMPs, even though the elevated levels of proMMP-2 and -9 would indicate a pro-inflammatory state in the brain. (C) 2010 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Central nervous system, Inflammation, Leishmania chagasi, MMP-2, MMP-9, Zymography
Como citar
Veterinary Immunology and Immunopathology. Amsterdam: Elsevier B.V., v. 136, n. 3-4, p. 340-345, 2010.