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dc.contributor.authorNeto, Luiz da Silveira
dc.contributor.authorVicente Sobrinho, Ludmila Silva
dc.contributor.authorMartins, Camille Oliveira
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorMarcondes, Mary [UNESP]
dc.contributor.authorFelix de Lima, Valeria Marcal [UNESP]
dc.date.accessioned2013-09-30T17:56:10Z
dc.date.accessioned2014-05-20T13:42:52Z
dc.date.available2013-09-30T17:56:10Z
dc.date.available2014-05-20T13:42:52Z
dc.date.issued2011-05-11
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2010.11.055
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 177, n. 3-4, p. 374-377, 2011.
dc.identifier.issn0304-4017
dc.identifier.urihttp://hdl.handle.net/11449/14898
dc.description.abstractVisceral leishmaniasis is a disease caused by Leishmania (Leishmania) chagasi and represents a serious public health problem. The dog is the main urban reservoir of the disease: however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. This study aimed to detect cats seropositive for Leishmania spp. using different antigens. Additional studies were performed using sera from cats with Toxoplasma gondii (n = 15) to evaluate cross-reactivity. Serum samples (n = 113) from cats living in the town of Aracatuba, State of São Paulo, Brazil, an endemic area for human and canine visceral leishmaniasis, were tested by indirect ELISA using different antigens: crude (CAG-ELISA), fucose-mannose ligand (FML-ELISA) and K39 (rK39-ELISA). Anti-Leishmania spp. antibodies were detected in 23.0% of samples evaluated by CAG-ELISA, 13.3% by FML-ELISA and 15.9% by RK39-ELISA. Only reactive sera in all three tests were considered truly positive. No disagreement occurred among the tests (p <0.05). Serum samples seropositive for toxoplasmosis tested by CAG-ELISA were negative, but one sample (6.7%) was positive for FML-ELISA and rK39-ELISA suggesting a cross-reaction between these antigens and anti-T. gondii antibodies. These findings indicate the occurrence of feline leishmaniasis in Aracatuba. Further studies are required to clarify the role of cats in the epidemiological cycle of leishmaniasis. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent374-377
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.sourceWeb of Science
dc.subjectDiagnosisen
dc.subjectCaten
dc.subjectLeishmaniasisen
dc.subjectSerologyen
dc.subjectToxoplasmaen
dc.titleUse of crude, FML and rK39 antigens in ELISA to detect anti-Leishmania spp. antibodies in Felis catusen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Clin Cirurgia & Reprod Anim, Fac Odontol Aracatuba,Curso Med Vet, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Patol Vet, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Clin Cirurgia & Reprod Anim, Fac Odontol Aracatuba,Curso Med Vet, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Patol Vet, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, Brazil
dc.identifier.doi10.1016/j.vetpar.2010.11.055
dc.identifier.wosWOS:000290696800025
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
dc.identifier.lattes1817946671090010
unesp.author.lattes1817946671090010
unesp.author.lattes3254990612451836
unesp.author.orcid0000-0001-6931-4479[5]
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
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