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dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.authorSantos, Vanessa A. C. [UNESP]
dc.date.accessioned2013-09-30T18:30:30Z
dc.date.accessioned2014-05-20T13:42:59Z
dc.date.available2013-09-30T18:30:30Z
dc.date.available2014-05-20T13:42:59Z
dc.date.issued2011-11-01
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2011.08.004
dc.identifier.citationJournal of Endodontics. New York: Elsevier B.V., v. 37, n. 11, p. 1520-1524, 2011.
dc.identifier.issn0099-2399
dc.identifier.urihttp://hdl.handle.net/11449/14954
dc.description.abstractIntroduction: The goal of this study was to evaluate the mechanism involved in the expression of fibroblast growth factor-2 (FGF-2) by odontoblast-like cells (ODs) stimulated by lipopolysaccharide (LPS) via p42/44, p38, and PI3K. Methods: ODs (MDPC-23) were stimulated with LPS for 1, 6, and 24 hours. The FGF-2 expression was evaluated by reverse transcriptase-polymerase chain reaction and protein production by Western blot analysis. Cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD98059, PD), p38 inhibitor (SB202190, SB), or PI3K inhibitor (wortmannin, Wort) and then stimulated with LPS (0.1 mu g/mL) for 1 hour. Results: LPS-stimulated ODs express FGF-2 in concentrations at 0.1, 1, 10, and 100 mu g/mL after 1 hour. DEX and MK were able to inhibit FGF-2 mRNA expression. PD, SB, and Wort also decreased expression. Conclusions: LPS-induced FGF-2 mRNA expression on ODs occurs via leukotriene production or cytokine and/or chemokine production activating p42/44, p38, and PI3K pathway. The data suggest that FGF-2 released by ODs might act as modulators of immune response mainly in the tissue repair. (J Endod 2011;37:1520-1524)en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1520-1524
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Endodontics
dc.sourceWeb of Science
dc.subjectChemokinesen
dc.subjectcytokinesen
dc.subjectfibroblast growth factoren
dc.subjectleukotrienesen
dc.subjectlipopolysaccharidesen
dc.subjectodontoblasten
dc.titleStudies on the Expression of Fibroblast Growth Factor-2 from Odontoblast-like Cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Estadual Paulista, Dept Basic Sci, Pharmacol Lab, Sch Dent Aracatuba, BR-16018805 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Pediat & Social Dent, Sch Dent Aracatuba, BR-16018805 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Basic Sci, Pharmacol Lab, Sch Dent Aracatuba, BR-16018805 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Pediat & Social Dent, Sch Dent Aracatuba, BR-16018805 São Paulo, Brazil
dc.identifier.doi10.1016/j.joen.2011.08.004
dc.identifier.wosWOS:000296944300007
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 06/50603-0
dc.description.sponsorshipIdFAPESP: 08/51486-2
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araçatubapt
unesp.author.orcid0000-0003-0805-1120[1]
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
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