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dc.contributor.authorBernabé, Daniel Galera [UNESP]
dc.contributor.authorTamae, Adriano C. [UNESP]
dc.contributor.authorBiasoli, Eder Ricardo [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.date.accessioned2013-09-30T18:29:19Z
dc.date.accessioned2014-05-20T13:43:05Z
dc.date.available2013-09-30T18:29:19Z
dc.date.available2014-05-20T13:43:05Z
dc.date.issued2011-03-01
dc.identifierhttp://dx.doi.org/10.1016/j.bbi.2010.12.012
dc.identifier.citationBrain Behavior and Immunity. San Diego: Academic Press Inc. Elsevier B.V., v. 25, n. 3, p. 574-583, 2011.
dc.identifier.issn0889-1591
dc.identifier.urihttp://hdl.handle.net/11449/14997
dc.description.abstractPatients with oral cancer can have high psychological distress levels, but the effects of stress-related hormones on oral cancer cells and possible mechanisms underlying these relationships are unknown. In this study, we have investigated the effects of stress-related hormones on interleukin-6 (IL-6) secretion and proliferation of oral squamous cell carcinoma (OSCC) cells. The effects of norepinephrine (NE), and cortisol were studied in SCC9. SCC15, and SCC25 cells and effects of isoproterenol in SCC9 and SCC25 cells. Real-time PCR studies revealed constitutive beta 1- and beta 2-adrenergic receptors (beta-ARs) expression in the SCC9. SCC15, and SCC25 cells. The results showed that NE and isoproterenol significantly enhanced IL-6 mRNA expression and protein production in supernatants of SCC9 and SCC25 cells. Physiological stress levels of NE and isoproterenol (10 mu M) at 1 h elicited the most robust IL-6 increase. Regarding IL-6 secretion, 10 mu M NE induced a 5-fold increase at 1 h, 3.7-fold increase at 6 h, and 3.2-fold at 24 h in SCC9 cells. These effects were blocked by the beta-adrenergic antagonist propranolol, supporting a role for beta-ARs in IL-6 secretion. The effects of cortisol varied according to the hormone concentration. Pharmacological concentrations of cortisol (1000 nM) inhibited IL-6 production by SCC9 and SCC25 cells. Cortisol dose that simulates stress conditions (10 nM) tended to increase IL-6 expression in SCC9 cells. Hormonal doses that simulate stress conditions (10 mu M NE, at 6 h in SCC9 and SCC15 cells and 10 nM cortisol, at 48 h in SCC15 cells) stimulated increased cell proliferation. Treatment of SCC9 cells with IL-6 neutralizing ab (10 mu g/mL) partially inhibited NE-induced proliferation. Finally, 20 OSCC biopsies were shown to express beta 1- and beta 2-ARs. These findings suggest that stress hormones can affect oral cancer cells behavior. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent574-583
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofBrain Behavior and Immunity
dc.sourceWeb of Science
dc.subjectPsychological stressen
dc.subjectSquamous cell carcinomaen
dc.subjectOral canceren
dc.subjectInterleukin-6en
dc.subjectBeta-adrenergic receptoren
dc.subjectNorepinephrineen
dc.subjectCortisolen
dc.titleStress hormones increase cell proliferation and regulates interleukin-6 secretion in human oral squamous cell carcinoma cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUNESP Univ Estadual Paulista, Oral Oncol Ctr, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Pathol & Clin Propedeut, Sch Dent Aracatuba, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Pharmacol Lab, Dept Basic Sci, Sch Dent Aracatuba, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Oral Oncol Ctr, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Pathol & Clin Propedeut, Sch Dent Aracatuba, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Pharmacol Lab, Dept Basic Sci, Sch Dent Aracatuba, São Paulo, Brazil
dc.identifier.doi10.1016/j.bbi.2010.12.012
dc.identifier.wosWOS:000287626600023
dc.rights.accessRightsAcesso aberto
dc.description.sponsorshipIdFAPESP: 06/59835-0
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araçatubapt
dc.identifier.fileWOS000287626600023.pdf
dc.identifier.lattes3846891167083211
dc.identifier.orcid0000-0002-5326-2026
unesp.author.lattes3846891167083211[3]
unesp.author.orcid0000-0003-0805-1120[4]
unesp.author.orcid0000-0002-1225-7749[1]
unesp.author.orcid0000-0002-5326-2026[3]
dc.relation.ispartofjcr6.306
dc.relation.ispartofsjr2,780
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