Caracterização de variação no número de cópias (CNV) no gene HMGA2 associado com tamanho de prepúcio em bovinos nelore (Bos indicus)
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Data
2018-06-21
Autores
Aguiar, Tamiris Sayuri
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Universidade Estadual Paulista (Unesp)
Resumo
O gene High Mobility Group AT-hook2 (HMGA2) apresentou fortes evidências de estar associado com o tamanho de umbigo em bovinos da raça Nelore através da análises de associação genômica ampla (GWAS). Diversos relatos de associação desse gene a fenótipos do âmbito da morfologia corporal existem para diferentes espécies, tais como altura em humanos, cães e equinos, e tamanho da orelha em suínos. Descobertas recentes demonstraram que o gene HMGA2 está associado avia metabólica de grande importância fisiológica e biológica que tem como um dos principais fatores o PLAG1 (Pleomorphic adenoma gene1), que está associado ao fator de crescimento semelhante à insulina 2 (IGF2), importante regulador do crescimento e da reprodução em bovinos. No presente trabalho, foi descritaa identificação e caracterização de variação no número de cópias (CNV) cromossomo 5 do cromossomo bovino, na região do gene HMGA2 que apresenta associação a característica de tamanho de umbigo. Análises da sequência completa do genoma de indivíduos Bos taurus e Bos indicus foram empregadas para caracterizar o CNV, sendo sua validação realizada através de PCR quantitativo (qPCR). Além disso, os resultados foram comparados com dados de sequência de animais africanos B. indicus evidenciando a origem zebuína do CNV.
The High Mobility Group AT-hook 2 (HMGA2) gene presented strong evidence of being associated with navel size in Nellore cattle through genome association analysis (GWAS). Several reports of association of this gene with phenotypes in the scope of body morphology exist for different species, such as height in humans, dogs and horses, and ear size in swine. Recent discoveries have shown that the HMGA2 gene is associated with a metabolic pathway of great physiological and biological importance that has as one of its main factors PLAG1 (Pleomorphic adenoma gene 1), which is associated with insulin-like growth factor 2 (IGF2), important regulator of growth and reproduction in cattle. In the present work, the identification and characterization of copy number variation (CNV) on chromosome 5 of the bovine chromosome in the region of the HMGA2 gene that has association with the navel size trait was described. Genome sequence analysis of Bos taurus and Bos indicus individuals was used to characterize the CNV, and its validation was performed using quantitative PCR (qPCR). In addition, the results were compared with sequence data from a African B.indicus animals evidencing the indicine origin of the CNV.
The High Mobility Group AT-hook 2 (HMGA2) gene presented strong evidence of being associated with navel size in Nellore cattle through genome association analysis (GWAS). Several reports of association of this gene with phenotypes in the scope of body morphology exist for different species, such as height in humans, dogs and horses, and ear size in swine. Recent discoveries have shown that the HMGA2 gene is associated with a metabolic pathway of great physiological and biological importance that has as one of its main factors PLAG1 (Pleomorphic adenoma gene 1), which is associated with insulin-like growth factor 2 (IGF2), important regulator of growth and reproduction in cattle. In the present work, the identification and characterization of copy number variation (CNV) on chromosome 5 of the bovine chromosome in the region of the HMGA2 gene that has association with the navel size trait was described. Genome sequence analysis of Bos taurus and Bos indicus individuals was used to characterize the CNV, and its validation was performed using quantitative PCR (qPCR). In addition, the results were compared with sequence data from a African B.indicus animals evidencing the indicine origin of the CNV.
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Palavras-chave
HMGA2, CNV, Umbigo, Bos taurus, Bos indicus, Bovinos, Bovine, Navel