Artigos - Patologia e Propedêutica Clínica - FOA

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Blastic plasmacytoid dendritic cell neoplasm: diagnostic difficulty and proposed flowchart for histopathological diagnosis
(2023-01-01) Castolde, Gabriel Taylor; Pontes, Alexandre Lizardo Lourenço; Cortopassi, Gabriel Macedo; Xavier-Júnior, José Cândido Caldeira [UNESP]; Centro Universitário Católico Unisalesiano Auxilium; Private Practice; Universidade Estadual Paulista (UNESP); Instituto de Patologia de Araçatuba
Applicability of the Milan system for reporting salivary gland cytopathology: A single Brazilian institution experience
(2023-05-01) Tokunaga, Juliana Akemi Dornellas; de Peres, Eduardo Augusto Souza; Camilo-Junior, Deolino João; Xavier-Junior, José Cândido Caldeira [UNESP]; Centro Universitário Católico Unisalesiano Auxilium; Pathology Institute of Araçatuba; Universidade Estadual Paulista (UNESP)
Introduction: The Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) emerged in 2015 as an attempt to establish protocols for a most appropriate follow-up and treatment of patients with salivary gland lesions. Through fine needle aspiration (FNA), a safe and minimally invasive way to obtain cytological samples, the lesion is classified into one of the six categories, which have different risks of malignancy (ROM) and, therefore, different management. Materials and Methods: FNA cytology procedures performed between January 2016 and June 2020 (54 months) at the Pathology Institute of Araçatuba, São Paulo, Brazil, were analyzed by two pathologists with more than 5 years of experience. The ROM for each of the diagnostic categories was determines and compared with the ROM expected by the MSRSGC. Results: A total of 99 FNA of salivary gland lesions were reviewed and retrospectively categorized. Histopathological follow-up was available for 58 of 96 patients (60.42%). The patients age ranged from 23 to 98 years with the mean of 58.15 ± 15.29 years. The parotid gland was the most affected (81.82%). The average size of the lesions was 2.59 cm. The ROM for each category was 16.67%, 0%, 0%, 2.86%, 50%, 100%, 100%, respectively. Conclusion: This is the first Brazilian study describing the application of the MSRSGC in the routine of a private Laboratory out of a cancer center service. Therefore, it is an effective method in the classification of salivary gland lesions, when associated with the MSRSGC, to determine the ROM and its appropriate treatment.
Value of Immunohistochemistry to Differentiate Digital Papillary Adenocarcinoma From Acral Hidradenoma With Papillary Structures
(2022-11-01) Xavier-Júnior, José Cândido Caldeira [UNESP]; Camilo-Júnior, Deolino Joaõ; Carneiro Dias, André Luiz; Marques Ferreira, Pedro Paulo; Abdalla Filho, Antonio Roberto; Brenn, Thomas; Centro Universitário Unisalesiano Auxilium; Institute of Aracątuba; Universidade Estadual Paulista (UNESP); Private Clinic; University of Calgary
Digital papillary adenocarcinoma is a malignant adnexal tumor with a predilection for acral sites. Hidradenoma is a benign solid and cystic sweat gland neoplasm with focal ductal and glandular differentiation and good outcomes. Hidradenomas can occur at acral sites and show papillary structures; for this reason, they are included in the differential diagnosis of digital papillary adenocarcinoma, and immunohistochemistry is a valuable tool in this scenario. We described a case of a 43-year-old man with an epithelial tumor showing papillary structures in the intermediate phalanx of the fourth finger. There was diffuse positivity for p63 and negativity for S100 protein, suggesting that this tumor was an acral hidradenoma with papillary structures.
Monitoring periodontal lesions and their effects during pregnancy: microbiological aspects of the oral cavity and amniotic fluid in pregnant ewes
(2023-01-01) Souza, Natália C. [UNESP]; Ramos, Thamiris N.M. [UNESP]; Borsanelli, Ana Carolina; Saraiva, Júlia R. [UNESP]; Ferreira, Evandro M.; Schweitzer, Christiane M. [UNESP]; Elerson, Gaetti-Jardim [UNESP]; Dutra, Iveraldo S. [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de Goiás (UFG); Universidade de São Paulo (USP)
Periodontitis affects the teeth supporting tissues, leading to tooth loss and damage to animal health. Evidence in humans suggests that oral microorganisms spread systemically, increasing the risk of pregnancy disorders such as miscarriage, prematurity, and low birth weight. This study aimed to verify whether periodontopathogenic microorganisms reach the transplacental unit, culminating in problems in pregnant ewes. After analyzing the oral cavity, 10 clinically healthy pregnant ewes (OGCH group) and 10 pregnant ewes with periodontitis (OGP group) were selected. The subgingival biofilm was collected for the polymerase chain reaction (PCR) test and amniotic fluid for both the PCR and interleukin (IL) analysis. Peripheral blood was collected for complete blood count, and analyses of IL-6, IL1-β, and tumor necrosis factor-α were performed. Placental fragments were collected to assess the inflammatory changes using optical microscopy. After giving birth, both the ewes and their lambs were weighed. On clinical examination, a positive correlation between bleeding and suppuration (correlation index – CI=0.54), suppuration and marginal gingivitis (CI=0.34), and marginal gingivitis and edema (CI=0.54) was observed. The weights of the ewes (p=0.013) and their respective lambs (p=0.04) in the OGP group were lower than those of their OGCH group counterparts. The hematological analysis revealed that the OGP group ewes showed a slight increase in the mean corpuscular volume (p=0.2447), segmented cells (p=0.3375), and eosinophils (p=0.3823) when compared with the OGCH group ewes, without a statistical difference. Regarding the microorganisms detected in the oral cavity, there was a significant difference between the occurrence of periodontal pockets and the presence of Fusobacterium necrophorum (p=0.0328), Porphyromonas asaccharolytica (p=0.0392), and the Mollicutes class (p=0.0352). Staphylococcus genus (p=0.9107) and Archaea domain (p=0.7245) were detected in the amniotic samples of both groups, without a significant difference, whereas P. asaccharolytica (p=0.2685) was only detected in one sample in the OGCH group. The expression of cytokine IL-6 in the OGP group differed significantly between the prepartum and postpartum periods (p=0.0039); moreover, it differed significantly in the postpartum period between the OGCH and OGP groups (p=0.0198). Histological examination showed a higher percentage of placental changes in the OGP group (70%) than in the OGCH group, such as the presence of macrophages, neutrophils, plasma cells, and multifocal areas of calcification. These results do not corroborate the hypothesis of dissemination of oral microorganisms to the placental unit, suggesting that it constitutes placental isolation in sheep.
Case report of breast implant-associated anaplastic large cell lymphoma confined by fibrous capsule
(Wiley-Blackwell, 2022-10-01) Xavier-Junior, J. C. C. [UNESP]; Camilo-Junior, D. J.; Katsuda, P. G.; Okida, R. C.; Pathol Inst Aracatuba; Ctr Univ Catolico Salesiano Auxilium Unisalesiano; Universidade Estadual Paulista (UNESP); Private Clin
Adnexal tumours with papillary structures on acral sites are not always digital papillary adenocarcinoma: report of one case of hidradenoma on acral site with papillae
(Wiley-Blackwell, 2022-10-01) Xavier Junior, J. C. C. [UNESP]; Camilo Junior, D. J.; Ferreira, P. P. M.; Dias, A. L. C.; Pathol Inst Aracatuba; Sch Med; Universidade Estadual Paulista (UNESP)
Influence of different root canal obturation materials in CBCT imaging: An in vitro evaluation
(2021-01-01) Salineiro, Fernanda Cristina Sales; Velasco, Solange Kobayashi; Barros, Fabiana Mesquita; Salzedas, Leda Maria Pescinini [UNESP]; Sales, Marcelo Augusto Oliveira; Cavalcanti, Marcelo Gusmão Paraiso; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP); Faculdade de Odontologia
Objective: This study evaluated the influence of canal obturation materials in the production of cone beam computed tomography (CBCT) images artifact, by means of image density analysis and comparison between four materials in central incisors and monoradicular premolar teeth. Materials and Methods: The teeth were submitted to endodontic instrumentation and divided into 5 groups: one control group (no endodontic filling) and four test groups each one filled by a different endodontic sealer (PulpCanal Sealer, AHPlus, Sealer26 and BCSealer). After CBCT scanning, the images were assessed to determine grayscale variations in three root thirds (objective analysis). The second (subjective) analysis compared the control group with two of the test groups in a randomized process. Results: In the objective analysis, Sealer26 and BCSealer showed statistical difference for minimum values, in comparison with another sealers, for both anterior and posterior teeth. For maximum values, only control group differed statistically from test groups. When comparing premolars and central incisors grayscale values by ANOVA analysis, a statistically significant difference was found mainly for minimum values. At the subjective analysis, for both anterior and posterior teeth, PulpCanal Sealer was the filling most frequently appointed by the observers as the one that produced more artifact interference. Conclusion: Endodontic fillings at posterior teeth performed similar behavior. Only PulpCanal Sealer on apical third presented higher maximum values in relation to others groups, which could represent more white brands. For anterior teeth, BC Sealer performed higher minimum values in relation to other endodontic fillings, which could represent more dark bands.
Dysplastic melanocytic nevus: Are molecular findings the key to the diagnosis?
(2022-10-01) Xavier-Junior, José Cândido Caldeira [UNESP]; Ocanha-Xavier, Juliana Polizel [UNESP]; Pathology Institute of Araçatuba; Centro Universitário Católico Salesiano Auxilium (Unisalesiano); Universidade Estadual Paulista (UNESP)
The primary differential diagnosis of melanoma is dysplastic nevus. Until now, the final diagnosis is based on histological findings. With modern techniques, pathologists receive very early melanocytic lesions, which do not fit all malignant criteria. In those cases, even the concurrence between specialists and intraobserver agreement is not good. A molecular test could be developed to improve the accuracy of melanocytic lesions diagnosis and help in challenging lesions. The objective of this study is to provide a literary review looking for molecular markers that characterize dysplastic nevi and could help surgical pathologists differentiate them from melanoma. Articles from PubMed presenting case series of dysplastic nevi and melanoma genomic analyses were considered. The search was conducted in PubMed looking for papers written in English, published in the ten years preceding April 2020. This review confirmed the absence of a pathognomonic molecular marker of dysplastic nevi. This is a heterogeneous group of lesions with an uncertain risk to become a melanoma. The molecular heterogeneity of dysplastic nevi, the variation of histological diagnostic criteria among services, and the diverse molecular techniques applied are challenging features that might hamper definitive diagnoses. However, currently, there appears to be limited value for molecular testing in the diagnosis of dysplastic nevi.
Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats
(2022-04-01) dos Santos, Damáris Raissa [UNESP]; Fiais, Gabriela Alice [UNESP]; de Oliveira Passos, Arthur [UNESP]; dos Santos, Luis Fernando Gadioli [UNESP]; Kayahara, Giseli Mitsuy [UNESP]; Crivelini, Marcelo Macedo [UNESP]; Matsushita, Doris Hissako [UNESP]; Antoniali, Cristina [UNESP]; Nakamune, Ana Cláudia de Melo Stevanato [UNESP]; Dornelles, Rita Cássia Menegati [UNESP]; Chaves-Neto, Antonio Hernandes [UNESP]; Universidade Estadual Paulista (UNESP)
The objective of this study was to investigate the effects of orchiectomy (ORX) and testosterone replacement therapy (TRT) on redox balance and function of salivary glands. Forty-five young adult male Wistar rats (3 months old) were either castrated bilaterally or underwent fictitious surgery (SHAM) and were subsequently distributed into 3 groups: SHAM, ORX, and TRT (castrated rats that received an intramuscular injection of testosterone cypionate 10 mg/kg/weekly). All treatments started 4 weeks after castration (4 months old) and lasted 4 weeks (5 months old). At the end of treatment, pilocarpine-induced salivary secretion was collected to analyze salivary flow rate and biochemistry composition, and the parotid (PG) and submandibular (SMG) glands were sampled for redox balance markers and histomorphometric analyses. ORX increased salivary flow rate, calcium, phosphate, and chloride, and decreased total protein and amylase, while not changing the salivary buffer capacity, pH, sodium, and potassium compared to SHAM. TRT restored all salivary parameters to SHAM values. ORX increased oxidative lipid and protein damage, total antioxidant capacity, and uric acid in both salivary glands compared to SHAM. Superoxide dismutase, catalase, and glutathione peroxidase activities were greater only in the SMG of the ORX group in relation to SHAM. ORX decreased duct and acini area, while increasing connective tissue in the PG. On the other hand, ORX reduced duct area and increased acini area in the SMG compared to SHAM. TRT restored the redox balance and histomorphometric parameters to close to SHAM values in both salivary glands. Orchiectomy-induced salivary gland dysfunction was characterized by an increase in the salivary flow rate and changes in the secretion of total protein, amylase, and electrolytes, which are key factors, considered important for maintaining oral health status. To sum up, orchiectomy impaired the redox balance of the salivary glands. Our results also showed that TRT reversed the oxidative damage, morphological alterations, and salivary gland dysfunction induced by orchiectomy. Therefore, these results suggest an important action of testosterone on the redox balance and secretory ability of salivary glands.
Impact of VDR and RXR expression in non-melanoma skin cancer pathogenesis
(2022-01-01) Ocanha-Xavier, Juliana P. [UNESP]; Xavier, José C. C. [UNESP]; Guimarães da Silva, Márcia [UNESP]; Marques, Mariângela E. A. [UNESP]; Universidade Estadual Paulista (UNESP); Private Clinic (JPOX Clinic); Araçatuba Institute of Pathology; Unisalesiano
1,25(OH)2D3, the active form of vitamin D, has been extensively studied for its putative protective activities against tumors. It does biological work by connecting to a nuclear receptor called VDR, which heterodimerizes itself to another nuclear receptor, RXR. The study observed differences in VDR and RXR expression in non-melanoma skin cancer a actinic keratosis and compared it with normal skin. We performed VDR and RXR immunohistochemistry of 76 controls (normal skin), 49 actinic keratosis, 99 basal cell carcinomas and 96 squamous cell carcinomas from formalin-fixed paraffin-embedded, resulting from surgical procedures. There was a clear pattern in the control group (p < 0.001), with the positivity of both receptors, VDR and RXR. Actinic keratosis differed from the basal cell carcinoma and control groups concerning RXR expression (p < 0.001). SCC was negative for both receptors, differing in all groups (p < 0.001). The site of positivity (nuclear, cytoplasmatic or both) of VDR differed between all groups (p < 0.001). To date, our series is the largest of VDR and RXR immunohistochemistry concerning non-melanoma skin cancer. Our findings reinforce the need to understand the pathways involving VDR and RXR to direct therapies and prevention manoeuvres.
Lepromatous leprosy with dermatofibroma features: colonization or morphological variant of histoid leprosy with epidermal induction?
(2022-01-01) Outi Pontes, Cinthia Maria; Lourenco Pontes, Alexandre Lizardo; Ocanha-Xavier, Juliana Polizel [UNESP]; Xavier-Júnior, José Cândido Caldeira [UNESP]; Private Clinic; Universidade Estadual Paulista (UNESP); Centro Universitário Católico Unisalesiano Auxilium; Pathology Institute of Araçatuba
Background: Leprosy is one of the main health problems in developing countries. It can show many different clinical presentations. Case report: A 37-yr-old woman with multiple reddish-brown papules on the lower and upper limbs, including the palms. The initial clinical impression was pityriasis lichenoides chronica. Biopsies were taken. The specimen from the left shin showed classical histological features of lepromatous leprosy. The specimen from the left thigh was similar to lipidized dermatofibroma showing epidermal hyperplasia with basal layer hyperpigmentation, a narrow Grenz zone, and spindle xanthomatous cells among dermal fibers. Fite-Faraco staining revealed many bacilli. Discussion: No matter the clinical presentation, in the presence of lipidized macrophages, Fite-Faraco staining (an inexpensive method available worldwide) should be performed to rule out leprosy, even in nonendemic areas or associated with a tumor.
Erythema gyratum repens after COVID vaccination
(2022-01-01) Chiquito, D.; Xavier-Junior, J. C.C. [UNESP]; Peres, G.; Lupi, O.; Policlínica Geral do Rio de Janeiro (PGRJ); Pathology Institute of Araçatuba; Centro Universitário Católico Salesiano Auxilium (Unisalesiano); Universidade Estadual Paulista (UNESP); University of South Wales; Federal University of the State of Rio de Janeiro (UNIRIO); Universidade Federal do Rio de Janeiro (UFRJ)
Thyroid FNA performed by cytopathologists accompanied by radiologists guiding the ultrasound provide high-level quality results: A retrospective observational study
(2022-06-01) Borges, Felipe Abrantkoski; Camilo-Júnior, Deolino João; Yamamoto, Fábio Massahito [UNESP]; Xavier-Júnior, José Cândido C. [UNESP]; Centro Universitário Católico Unisalesiano Auxilium; Instituto de Patologia de Araçatuba (IPAT); Universidade Estadual Paulista (UNESP)
Background: On thyroid cytology, false negative and false positive rates are considered parameters for quality control. Nevertheless, there are few studies about medical professional involved in this procedure. We conducted a 4-year cytohistological correlation of thyroid specimens in a single institution. To describe cytological and histological correlation where radiologists guide the ultrasound (US) and cytopathologists perform the thyroid fine needle aspiration (FNA). Methods: Retrospective observational study of thyroid specimens in a Brazilian countryside setting. The ultrasound was performed by radiologists, and the FNA was performed by pathologists. All cases were reviewed by two pathologists following the Bethesda Reporting System for Reporting Thyroid Cytopathology and the World Health Organization's Classification of Tumours of Endocrine Organs. Clinical information, such as sex, age, location, and ultrasound data, was collected from our reports. Results: From a total of 3265 patients, 3703 nodules (391 patients showed more than one nodule) were submitted to FNA. For correlation, there were 168 surgical thyroidectomy specimens. The risk of malignancy for the Bethesda system categories were: nondiagnostic/unsatisfactory = 0/4 (0%); benign = 1/38 (2.6%); atypia of undetermined significance or follicular lesion of undetermined significance = 2/20 (10.0%); follicular neoplasm or suspicious for follicular neoplasm = 3/16 (18.7%); suspicious for malignancy = 63/67 (94.0%); and malignant = 22/23 (95.6%). Conclusion: High-level quality results can be accomplished with cooperation between cytopathologists performing thyroid FNA accompanied by radiologists guiding the ultrasound.
Findings other than age that help identify possible cases of non-invasive follicular thyroid neoplasm with nuclear characteristics of the papillary carcinoma type
(2022-02-01) Yamamoto, Fábio Massahito [UNESP]; Xavier-Junior, José Cândido Caldeira [UNESP]; Universidade Estadual Paulista (UNESP); Pathology Institute of Araçatuba; Centro Universitário Católico Salesiano Auxilium (Unisalesiano)
This section of the Journal of Dental Research, devoted to rapidly published short research notes, is being supported by the William J. Gies Foundation for the Advancement of Dentistry, Inc., New York, New York: Placement of a Rubber Dam on Rat Molars
(1967-01-01) Samfaio, Percy; Faculdade de Farmácia e Odontologia de Araçatuba
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose
(2013-10-23) Carvalho, A. A.F.; Biffe, B. G.; Nakamune, Ana Cláudia de Melo Stevanato [UNESP]; Louzada, M. J.Q.; Araçatuba Dental School; Universidade Federal de São Carlos (UFSCar); Medicine Veterinarian School at Araçatuba
Food restriction reduces body weight and influence bone mass and also is correlated with bone mineral density (BMD). Mechanisms have been proposed for the loss of BMD after body weight reduction, including reduced energy intake. Growing 8 wk-old Wistar male rats were randomly divided into Control and Calorie restriction associated with sucrose 30% (CRS). These animals were subjected to intermittent food restriction during 8 weeks and had free access to tap water and sucrose30% in distilled water. The rats were euthanized at the end of week 8, blood collected from abdominal aorta artery, femurs cleaned of adherent soft tissues, scanned using dual energy X-ray absorptiometry, structural and material properties determined by three-point bending testing in the mid-diaphyseal region, bone surface tested in a microhardness tester and microstructure was assessed in a microcomputer tomography. In CRS animals body weight decreased significantly relative to the Control animals. There was a clear option for high-sucrose beverage in CRS animals. No difference was observed in biochemical, densitometric and biomechanical analyzes. Results from micro CT showed only significant difference in connectivity of trabecular bone. It has been suggested that rats submitted to food restriction consumed sugar not because of its inherent palatability, but in order to alter their macronutrient balance and animals need to meet energy demands in high-sucrose.
Angioleiomyoma affecting the lips: Report of 3 cases and review of the literature
(2011-07-01) Gueiros, Luiz-Alcino; Romañach, Mário-José; Pires-Soubhia, Ana-Maria; Pires, Fábio-Ramôa; Paes-de-Almeida, Oslei; Vargas, Pablo-Agustin; Universidade Estadual de Campinas (UNICAMP); Júlio Mesquista State University; Estácio de Sá University
Angioleiomyoma is an uncommon benign soft tissue tumor usually found in the lower extremities and rarely observed in oral tissues. It is microscopically characterized as a proliferation of smooth muscle cells intermingled with abundant vascular channels. Oral angioleiomyomas affect mostly the lips, palate, buccal mucosa and tongue, and appears as a submucosal painless nodule. Upper lip is seldom affected and only few cases have been reported. We report three additional cases of angioleiomyoma affecting the lips of elderly patients. All lesions were asymptomatic and presented as submucosal nodules of approximately 1cm. Microscopic analysis on H&E sections revealed similar pattern in all cases, showing well-circumscribed and encapsulated tumors characterized by proliferation of smooth muscle cells and large amount of wide vascular spaces of varying sizes. Most tumor cells were immunoreactive for α-smooth muscle actin, desmin and HHF-35. CD34 was also positive on the endothelial cells. All patients were surgically treated and no recurrence was observed so far. The oral pathologists and clinicians should consider this entity when assessing nodular lesions on upper lip. © Medicina Oral S. L.
Evaluation of the apical infiltration after root canal disruption and obturation
(Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru, 2008) Gomes-Filho, Joao Eduardo [UNESP]; Hopp, Renato Nicolas [UNESP]; Bernabe, Pedro Felicio Estrada [UNESP]; Nery, Mauro Juvenal [UNESP]; Otoboni Filho, José Arlindo [UNESP]; Dezan Júnior, Eloi [UNESP]; Universidade de São Paulo (USP); Universidade Estadual Paulista (Unesp)
The aim of this study was to evaluate two root canal filling techniques used in teeth that had their apical foramen disrupted and compare the apical infiltration with an ideal clinical situation. Twenty-seven freshly extracted single-rooted teeth were selected and radiographed to confirm the existence of a single and straight root canal. The crowns were removed at a mean distance of 11 mm from the apex. The teeth had the root canals instrumented and were randomly assigned to 3 groups (n=9): ND group - root canals were filled using the lateral compaction technique and no disruption was performed; DRF group - the apical constriction was disrupted by advancing a #40 K-file 1 mm beyond the original working length, the canals were reinstrumented to create an apical ledge at 1 mm from the apical foramen and were obturated with a master gutta-percha cone with same size as the last file used for reinstrumentation; DF group - the teeth had the apical constriction disrupted and the canals were obturated with a master gutta-percha cone that fit at 1 mm from the apex. The teeth were submitted to dye leakage test with Rhodamine B for 7 days, using vaccum on the initial 5 min. The teeth were sectioned longitudinally and the leakage was measured in a linear fashion from apex to crown. There was no statistically significant difference (p>0.05) between the groups that had the apical foramen disrupted (DF, DRF), but significant difference was found between the disrupted groups and the non-disrupted one (p<0.01). In conclusion, none of the evaluated techniques was able to prevent apical infiltration, so working length so the working length determination has to be established and maintained carefully.
Basaloid squamous carcinoma of the oral cavity. Report of a case
(1993-01-01) de Araújo, Vera Cavalcanti; Biazolla, Eder Ricardo; Moraes, Norberto Perri; Furuse, Takeo Adhemar; Melhado, Ronaldo Maia; Universidade de São Paulo (USP)
A case of basaloid squamous carcinoma that involves the posterior buccal mucosa is described. The major histopathologic feature is a carcinoma with a basaloid pattern in association with squamous differentiation. The basaloid cells exhibit large and vesicular nuclei and eosinophilic clear or vacuolated cytoplasm. Cells are distributed in cords, trabeculae, or lobules that occasionally show glandular arrangement. The majority of the tumor cells are positive for keratin and a large group of cells distributed in glandular arrangement are positive also for vimentin. © 1993.
EFEITOS DA DEXAMETASONA 21 FOSFATO SOBRE AS GLANDULAS SALIVARES DE RATOS. ESTUDO HISTOQUIMICO
(1974-12-01) Melhado, R. M.; Martinelli, C.
The parotid, submandibular and sublingual glands of 12 male rats injected with dexamethasone 21 phosphate (0.20 mg per kg body weight) were removed and studied. Identical procedures were used in 12 male rats injected with 0.2 ml of distilled water (control group). All glands were stained by hematoxylin and eosin, Masson, periodic acid Schiff, chromic acid Schiff, Hale's colloidal iron, Alcian blue (pH 1.0 and 2.5), ninhydrin Schiff, ferric ferricyanide and gallocyanin chrome alum. Ribonuclease, testicular hyaluronidase, α amylase and blockade by acid hydrolysis were used. Dexamethasone caused: increased glycogen in submandibular (striated and granular) ducts and sublingual striated ducts; increased sulfhydril groups and RNA in parotid acini; increased amino groups in submandibular and parotid glands; increased sialic acid in sublingual glands (acini and crescent formations) and submandibular acini. (43 references).

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