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dc.contributor.authorde Mendonca, Adrian Melo
dc.contributor.authorde Oliveira, Camila Favero
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.date.accessioned2013-09-30T18:31:26Z
dc.date.accessioned2014-05-20T13:44:56Z
dc.date.available2013-09-30T18:31:26Z
dc.date.available2014-05-20T13:44:56Z
dc.date.issued2011-08-01
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22016918
dc.identifier.citationAmerican Journal of Dentistry. Weston: Mosher & Linder, Inc, v. 24, n. 4, p. 233-238, 2011.
dc.identifier.issn0894-8275
dc.identifier.urihttp://hdl.handle.net/11449/15769
dc.description.abstractPurpose: To evaluate the cytotoxicity of a self-etch resin-based luting cement, RelyXUnicem (RXU) upon chemical or dual cure and with or without interposition of IPS d.SIGN (IPSD) or IPS Empress II (IPSE) ceramic discs between cement and light source. Methods: 112 RXU specimens were subjected to different curing conditions and incubated in culture medium (DMEM) to obtain extracts. The following groups were formed: G1: DMEM (control); G2: dual RXU; G3: chemical RXU; G4: dual RXU+IPSD; G5: chemical RXU+IPSD; G6: dual RXU+IPSE; and G7: chemical RXU+IPSE. Cultured odontoblast-like cells were incubated for 24 hours in contact with the extracts. Data from cell metabolism (CM), total protein dosage (TPD) and alkaline phosphatase activity (APA) were obtained and analyzed statistically (alpha= 0.05; Kruskal Wallis and Mann-Whitney tests). Cell morphology was analyzed by SEM. Results: CM and APA were significantly lower in G3 and G7 than in G1 (P< 0.05). Significant TPD decrease occurred in G5 and G7 compared to G1 (P< 0.05). Only G4 and G6 presented CM changes. RXU caused no cytotoxicity when subjected to dual cure without ceramic interposition. However, mild cytopathic effects were observed after chemical setting without ceramic interposition, and after chemical and dual activation under ceramic discs. (Am J Dent 2011;24:233-238).en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent233-238
dc.language.isoeng
dc.publisherMosher & Linder, Inc
dc.relation.ispartofAmerican Journal of Dentistry
dc.sourceWeb of Science
dc.titleInfluence of the activation mode of a self-etch resin-based luting cement upon the metabolism of odontoblast-like cellsen
dc.typeArtigo
dcterms.rightsHolderMosher & Linder, Inc
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationPaulista State Univ UNESP, Dept Physiol & Pathol, Fac Dent Araraquara, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationPaulista State Univ UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespPaulista State Univ UNESP, Dept Physiol & Pathol, Fac Dent Araraquara, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespPaulista State Univ UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil
dc.identifier.wosWOS:000295652800008
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 07/50646-3
dc.description.sponsorshipIdCNPq: 305204/2010-6
dc.description.sponsorshipIdCNPq: 30129/2010-1
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
dc.identifier.lattes4517484241515548
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7455-6867[4]
dc.relation.ispartofjcr0.760
dc.relation.ispartofsjr0,509
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