Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections
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Data
2018
Autores
Bolaños, Carmen Alicia Daza
Franco, Marília Masello Junqueira
Souza Filho, Antonio Francisco
Ikuta, Cássia Yumi
Burbano-rosero, Edith Mariela
Ferreira Neto, José Soares
Heinemann, Marcos Bryan
Motta, Rodrigo Garcia
Paula, Carolina Lechinski De
Morais, Amanda Bonalume Cordeiro De
Título da Revista
ISSN da Revista
Título de Volume
Editor
Instituto de Medicina Tropical
Resumo
ABSTRACT Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days. Colonies compatible with mycobacteria were stained by Ziehl-Neelsen to detect acid-fast bacilli, while to confirm the Mycobacterium genus, conventional PCR was performed. Fourteen mycobacterial strains were isolated from 12 cows (8.4%). The hsp65 gene sequencing identified M. engbaekii (n=5), M. arupense (n=4), M. nonchromogenicum (n=3), and M. heraklionense (n=2) species belong to the Mycobacterium terrae complex. Despite the absence of M. tuberculosis complex species in the milk samples, identification of these mycobacteria highlights the risk of pathogen transmission from bovines to humans throughout milk or dairy products, since many of mycobacterial species described here have been reported in pulmonary and extrapulmonary diseases both in immunocompetent and immunocompromised people.
Descrição
Palavras-chave
M. terrae complex, Bovine, Intradermal tuberculin tests, Sequencing
Como citar
Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical, v. 60, p. -, 2018.