Probiótico administrado em embriões e pintos de frangos de corte na redução da colonização por Salmonella Heidelberg e integridade entérica
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Data
2018-11-01
Autores
Martins, Bruna Boaro
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Objetivou-se avaliar o efeito de probiótico administrado in ovo ou spray sobre a histomorfometria, contagem de células caliciformes (CC), avaliação ultraestrutural da mucosa entérica e quanto à capacidade de redução de Salmonella Heidelberg (SH) em órgãos e no conteúdo cecal de frangos de corte desafiados experimentalmente com SH. Para histomorfometria, CC, avaliação ultraestrutural da mucosa entérica foram utilizados 297 frangos de corte Cobb®, distribuídos em delineamento inteiramente casualizado em esquema fatorial (2x2)+2, com 9 repetições. Para as análises microbiológicas, foram utilizados 162 frangos de corte Cobb®, distribuídos em DIC com 3 tratamentos (T3, T4 e T5), com 9 repetições de 6 aves cada. T1: aves tratadas in ovo com probiótico; T2: aves tratadas por spray com probiótico; T3: aves tratadas in ovo com probiótico e desafiadas com SH; T4: aves tratadas por spray com probiótico e desafiadas com SH; T5: aves não tratadas com probiótico e desafiadas com SH; T6: aves não tratadas com probiótico e não desafiadas com SH. No 2º dia de vida, as aves infectadas foram anilhadas e alojadas junto às aves dos tratamentos T3, T4 e T5. Para contagem de SH em órgãos e no conteúdo cecal aos 3, 5, 7, 14 e 21 dias pós-desafio e para análise histomorfométrica, CC e avaliação ultraestrutural nos 5, 9, 16 e 23 dias de vida das aves, 5 aves de cada tratamento mais 1 ave anilhada, dos tratamentos T3, T4 e T5 foram eutanasiadas. Os dados microbiológicos foram submetidos ao teste de Kruskal-Wallis ranks, enquanto que os dados de histomorfometria e CC foram submetidos à análise de variância. Aos 21 dias pós-desafio para o conteúdo cecal, foi possível observar que aves do T4 apresentaram menor contaminação quando comparado com as aves do T3 e T5. Para os parâmetros histomorfométrico e CC, o probiótico apresentou ligeira melhora nas medidas em todas as idades e segmentos estudados. A integridade da mucosa ileal mostrou-se mais preservada, e a colonização com agente trófico foi mais intensa na mucosa cecal das aves do T2 e T4. O probiótico destaca-se como importante alternativa ao uso de antibióticos para controle de SH em frangos e corte, pois houve redução da contaminação no conteúdo cecal, melhora a integridade da mucosa entérica de frangos de corte, e mostra maior eficácia em estabelecer microbiota protetora capaz de preservar a ultraestrutura intestinal quando submetido a desafio com SH, sendo que a via de aplicação spray apresenta maior potencial de atuação.
The aim of this study was evaluate the effect of probiotic administered in ovo or spray on histomorphometry, goblet cell count (CC), ultrastructural evaluation of the enteric mucosa and the ability to reduce Salmonella Heidelberg (SH) in organs and cecal content in broilers challenged experimentally with SH. For the histomorphometry, CC, ultrastructural evaluation of the enteric mucosa, 297 Cobb® broilers were used, distributed in a completely randomized design in a factorial (2x2) +2, with 9 replicates. For the microbiological analyzes, 162 Cobb® broilers were used, distributed in completely randomized desig with 3 treatments (T3, T4 and T5), with 9 replicates of 6 birds each. T1: birds treated in ovo with probiotic; T2: birds treated with probiotic by spray; T3: birds treated in ovo with probiotic and challenged with SH; T4: birds treated with probiotic by spray and challenged with SH; T5: birds not treated with probiotic and challenged with SH; T6: birds not treated with probiotic and not challenged with SH. On the 2nd day of life, the infected birds were ringed and housed with birds from T3, T4 and T5 treatments. For SH count in organs and cecal content at 3, 5, 7, 14 and 21 days post challenge and for histomorphometric analysis, CC and ultrastructural evaluation at 5, 9, 16 and 23 days of life of the birds, 5 birds of each treatment plus one ringed bird, T3, T4 and T5 treatments were euthanized. The microbiological data were submitted to the Kruskal-Wallis ranks test, while the histomorphometric and CC data were submitted to analysis of variance. At 21 days post challenge for cecal content, it was possible to observe that T4 birds presented lower contamination when compared to the birds of T3 and T5. For the histomorphometric and CC parameters, the probiotic had a slight improvement in measurements in all ages and segments studied. The integrity of the ileal mucosa was more preserved, and the colonization with trophic agent was more intense in the cecal mucosa of the birds of T2 and T4. The probiotic is an important alternative to use of antibiotics for control SH in broilers, because there was a reduction in contamination in the cecal content, it improves the integrity of enteric mucosa of broilers, and it shows a greater efficacy in establishing a protective microbiota of preserving the intestinal ultrastructure when challenged with SH, with the spray application route showing greater potential for action.
The aim of this study was evaluate the effect of probiotic administered in ovo or spray on histomorphometry, goblet cell count (CC), ultrastructural evaluation of the enteric mucosa and the ability to reduce Salmonella Heidelberg (SH) in organs and cecal content in broilers challenged experimentally with SH. For the histomorphometry, CC, ultrastructural evaluation of the enteric mucosa, 297 Cobb® broilers were used, distributed in a completely randomized design in a factorial (2x2) +2, with 9 replicates. For the microbiological analyzes, 162 Cobb® broilers were used, distributed in completely randomized desig with 3 treatments (T3, T4 and T5), with 9 replicates of 6 birds each. T1: birds treated in ovo with probiotic; T2: birds treated with probiotic by spray; T3: birds treated in ovo with probiotic and challenged with SH; T4: birds treated with probiotic by spray and challenged with SH; T5: birds not treated with probiotic and challenged with SH; T6: birds not treated with probiotic and not challenged with SH. On the 2nd day of life, the infected birds were ringed and housed with birds from T3, T4 and T5 treatments. For SH count in organs and cecal content at 3, 5, 7, 14 and 21 days post challenge and for histomorphometric analysis, CC and ultrastructural evaluation at 5, 9, 16 and 23 days of life of the birds, 5 birds of each treatment plus one ringed bird, T3, T4 and T5 treatments were euthanized. The microbiological data were submitted to the Kruskal-Wallis ranks test, while the histomorphometric and CC data were submitted to analysis of variance. At 21 days post challenge for cecal content, it was possible to observe that T4 birds presented lower contamination when compared to the birds of T3 and T5. For the histomorphometric and CC parameters, the probiotic had a slight improvement in measurements in all ages and segments studied. The integrity of the ileal mucosa was more preserved, and the colonization with trophic agent was more intense in the cecal mucosa of the birds of T2 and T4. The probiotic is an important alternative to use of antibiotics for control SH in broilers, because there was a reduction in contamination in the cecal content, it improves the integrity of enteric mucosa of broilers, and it shows a greater efficacy in establishing a protective microbiota of preserving the intestinal ultrastructure when challenged with SH, with the spray application route showing greater potential for action.
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Palavras-chave
agente trófico, exclusão competitiva, in ovo, microbiológico, saúde intestinal, trophic agent, competitive exclusion, microbiological, intestinal health